Characterisation of specific activities and hydrolytic properties of cell-wall-degrading enzymes produced by Trichoderma reesei Rut C30 on different carbon sources

Bálint Sipos (Corresponding Author), Zsuzsa Bénko, Dora Dienes, Kati Réczey, Liisa Viikari, Matti Siika-aho

    Research output: Contribution to journalArticleScientificpeer-review

    72 Citations (Scopus)

    Abstract

    Conversion of lignocellulosic substrates is limited by several factors, in terms of both the enzymes and the substrates. Better understanding of the hydrolysis mechanisms and the factors determining their performance is crucial for commercial lignocelluloses-based processes. Enzymes produced on various carbon sources (Solka Floc 200, lactose and steam-pre-treated corn stover) by Trichoderma reesei Rut C30 were characterised by their enzyme profile and hydrolytic performance. The results showed that there was a clear correlation between the secreted amount of xylanase and mannanase enzymes and that their production was induced by the presence of xylan in the carbon source. Co-secretion of α-arabinosidase and α-galactosidase was also observed. Secretion of β-glucosidase was found to be clearly dependent on the composition of the carbon source, and in the case of lactose, 2-fold higher specific activity was observed compared to Solka Floc and steam-pre-treated corn stover. Hydrolysis experiments showed a clear connection between glucan and xylan conversion and highlighted the importance of β-glucosidase and xylanase activities. When hydrolysis was performed using additional purified β-glucosidase and xylanase, the addition of β-glucosidase was found to significantly improve both the xylan and glucan conversion.
    Original languageEnglish
    Pages (from-to)347-364
    Number of pages18
    JournalApplied Biochemistry and Biotechnology
    Volume161
    Issue number1-8
    DOIs
    Publication statusPublished - 2010
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Trichoderma reesei Rut C30
    • Cellulase fermentation
    • Enzymatic hydrolysis
    • Accessory enzymes
    • Hemicellulases
    • beta-Glucosidase

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