Characterisation of steryl esterase activities in commercial lipase preparations

Hanna Kontkanen (Corresponding Author), Maija Tenkanen, Richard Fagerström, Tapani Reinikainen

Research output: Contribution to journalArticleScientificpeer-review

50 Citations (Scopus)

Abstract

Triglycerides, steryl esters, resin acids, free fatty acids and sterols are lipophilic extractives of wood (commonly referred to as pitch or wood resin) and have a negative impact on paper machine runnability and quality of paper. Thus, enzymes capable of modifying these compounds would be potential tools for reducing pitch problems during paper manufacture. In this work, 19 commercial lipase preparations were tested for their ability to degrade steryl esters, which may play a significant role in the formation and stabilisation of pitch particles. Six lipase preparations were shown to be able to degrade steryl esters. Lipase preparations of Pseudomonas sp., Chromobacterium viscosum and Candida rugosa were shown to have the highest steryl esterase activities. The enzymes were able to hydrolyse steryl esters totally in the presence of a surfactant (Thesit). Up to 80% of the steryl esters were degraded in aqueous dispersion. Preliminary characterisation of the enzymatic activities revealed that the lipase preparation of Pseudomonas sp. could be the most potential enzyme in industrial applications. The steryl esterase activity of this preparation was stable over a broad pH range and the enzyme was able to act efficiently at pH 6–10 and at temperatures up to 70 °C.
Original languageEnglish
Pages (from-to)51 - 59
Number of pages9
JournalJournal of Biotechnology
Volume108
Issue number1
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Lipases
Esterases
Lipase
Esters
Enzymes
Pseudomonas
Wood
Resins
Chromobacterium
Candida
Sterols
Fatty acids
Nonesterified Fatty Acids
Surface-Active Agents
Industrial applications
Triglycerides
Surface active agents
Stabilization
Temperature
Acids

Keywords

  • Steryl esters
  • Steryl esterase
  • Cholesteryl esterase
  • Lipase
  • (Wood) extractives
  • Pitch problems

Cite this

Kontkanen, Hanna ; Tenkanen, Maija ; Fagerström, Richard ; Reinikainen, Tapani. / Characterisation of steryl esterase activities in commercial lipase preparations. In: Journal of Biotechnology. 2004 ; Vol. 108, No. 1. pp. 51 - 59.
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abstract = "Triglycerides, steryl esters, resin acids, free fatty acids and sterols are lipophilic extractives of wood (commonly referred to as pitch or wood resin) and have a negative impact on paper machine runnability and quality of paper. Thus, enzymes capable of modifying these compounds would be potential tools for reducing pitch problems during paper manufacture. In this work, 19 commercial lipase preparations were tested for their ability to degrade steryl esters, which may play a significant role in the formation and stabilisation of pitch particles. Six lipase preparations were shown to be able to degrade steryl esters. Lipase preparations of Pseudomonas sp., Chromobacterium viscosum and Candida rugosa were shown to have the highest steryl esterase activities. The enzymes were able to hydrolyse steryl esters totally in the presence of a surfactant (Thesit). Up to 80{\%} of the steryl esters were degraded in aqueous dispersion. Preliminary characterisation of the enzymatic activities revealed that the lipase preparation of Pseudomonas sp. could be the most potential enzyme in industrial applications. The steryl esterase activity of this preparation was stable over a broad pH range and the enzyme was able to act efficiently at pH 6–10 and at temperatures up to 70 °C.",
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Characterisation of steryl esterase activities in commercial lipase preparations. / Kontkanen, Hanna (Corresponding Author); Tenkanen, Maija; Fagerström, Richard; Reinikainen, Tapani.

In: Journal of Biotechnology, Vol. 108, No. 1, 2004, p. 51 - 59.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterisation of steryl esterase activities in commercial lipase preparations

AU - Kontkanen, Hanna

AU - Tenkanen, Maija

AU - Fagerström, Richard

AU - Reinikainen, Tapani

PY - 2004

Y1 - 2004

N2 - Triglycerides, steryl esters, resin acids, free fatty acids and sterols are lipophilic extractives of wood (commonly referred to as pitch or wood resin) and have a negative impact on paper machine runnability and quality of paper. Thus, enzymes capable of modifying these compounds would be potential tools for reducing pitch problems during paper manufacture. In this work, 19 commercial lipase preparations were tested for their ability to degrade steryl esters, which may play a significant role in the formation and stabilisation of pitch particles. Six lipase preparations were shown to be able to degrade steryl esters. Lipase preparations of Pseudomonas sp., Chromobacterium viscosum and Candida rugosa were shown to have the highest steryl esterase activities. The enzymes were able to hydrolyse steryl esters totally in the presence of a surfactant (Thesit). Up to 80% of the steryl esters were degraded in aqueous dispersion. Preliminary characterisation of the enzymatic activities revealed that the lipase preparation of Pseudomonas sp. could be the most potential enzyme in industrial applications. The steryl esterase activity of this preparation was stable over a broad pH range and the enzyme was able to act efficiently at pH 6–10 and at temperatures up to 70 °C.

AB - Triglycerides, steryl esters, resin acids, free fatty acids and sterols are lipophilic extractives of wood (commonly referred to as pitch or wood resin) and have a negative impact on paper machine runnability and quality of paper. Thus, enzymes capable of modifying these compounds would be potential tools for reducing pitch problems during paper manufacture. In this work, 19 commercial lipase preparations were tested for their ability to degrade steryl esters, which may play a significant role in the formation and stabilisation of pitch particles. Six lipase preparations were shown to be able to degrade steryl esters. Lipase preparations of Pseudomonas sp., Chromobacterium viscosum and Candida rugosa were shown to have the highest steryl esterase activities. The enzymes were able to hydrolyse steryl esters totally in the presence of a surfactant (Thesit). Up to 80% of the steryl esters were degraded in aqueous dispersion. Preliminary characterisation of the enzymatic activities revealed that the lipase preparation of Pseudomonas sp. could be the most potential enzyme in industrial applications. The steryl esterase activity of this preparation was stable over a broad pH range and the enzyme was able to act efficiently at pH 6–10 and at temperatures up to 70 °C.

KW - Steryl esters

KW - Steryl esterase

KW - Cholesteryl esterase

KW - Lipase

KW - (Wood) extractives

KW - Pitch problems

U2 - 10.1016/j.jbiotec.2003.11.003

DO - 10.1016/j.jbiotec.2003.11.003

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VL - 108

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