Characterisation of the translation initiation factors of Trichoderma reesei

Anne Huuskonen, Edward Alatalo, Markku Saloheimo, Merja Penttilä, Joop van der Laan

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

    Abstract

    Protein translation is regulated in eukaryotes in response to various stress conditions. Inhibition of protein secretion leads to reduced translation initiation rates in mammalian cells. Our aim has been to characterise translation initiation factors of the filamentous fungus Trichoderma reesei both at gene and protein levels and to address possible feedback control between secretion and translation initiation in filamentous fungi. The genes encoding the translation initiation factors eIF2alpha, eIF2beta and eIF4A have been isolated and sequenced from T. reesei. Based on the results from the Southern hybridisation all three genes are present in the Trichoderma genome in one copy. The eIF2beta gene contains three introns which can be differentially spliced generating a population of mRNAs with different ORFs. The expression levels of the eIF genes have been examined in mycelia treated with different chemical agents known to have influence on protein translation and/or secretion. These agents include dithiotreitol (DTT) and Brefeldin A (BFA). Of the chemicals studied the reducing agent DTT appears to have the most drastic effect on all the three genes. DTT's effect is most pronounced in the case of the eIF2beta where the drug has been shown to alter the ratio of the different mRNA forms. The effects of the different drugs on the translation initiation factor protein levels has also been under investigation. To obtain more information on the effects of the eIF2alpha, one of the key regulatory factors in translation initiation, two different strains expressing a mutant form of this gene have been constructed. One strain is expressing the non-phosphorylative form of this protein and the other strain mimics the constitutively phosphorylated form of the protein. The mutations introduced to the gene appear to have only minor effects on the protein production and growth, possibly on the germination stage.
    Original languageEnglish
    Title of host publicationFungal Genetics Conference (2001)
    Subtitle of host publicationXXI Fungal Genetics Conference Abstracts
    Publication statusPublished - 2001
    MoE publication typeNot Eligible
    EventXXI Fungal Genetics Conference - Asilomar, United States
    Duration: 13 Mar 200118 Mar 2001
    http://newprairiepress.org/cgi/viewcontent.cgi?article=1182&context=fgr (View abstracts)

    Publication series

    SeriesFungal Genetics Reports
    NumberArticle 17
    Volume48
    ISSN1941-4757

    Conference

    ConferenceXXI Fungal Genetics Conference
    Country/TerritoryUnited States
    CityAsilomar
    Period13/03/0118/03/01
    Internet address

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