Characterisation of two 14-3-3 genes from Trichoderma reesei

Interactions with yeast secretory pathway components

Tuija Vasara, Sirkka Keränen, Merja Penttilä, Markku Saloheimo

Research output: Contribution to journalArticleScientificpeer-review

19 Citations (Scopus)

Abstract

The 14-3-3 proteins are highly conserved, ubiquitously expressed proteins taking part in numerous cellular processes. Two genes encoding 14-3-3 proteins, ftt1 and ftt2, were isolated and characterised from the filamentous fungus Trichoderma reesei. FTTI showed the highest sequence identity (98% at the amino acid level) to the Trichoderma harzianum protein Th1433. FTTII is relatively distinct from FTTI, showing approximately 75% identity to other fungal 14-3-3 proteins. Despite their sequence divergence, both of the T. reesei ftt genes were equally able to complement the yeast bmh1 bmh2 double disruption. The T. reesei ftt genes were also found to be quite closely linked in the genomic DNA. A C-terminally truncated version of ftt1 (ftt1ΔC) was first isolated as a multicopy suppressor of the growth defect of the temperature-sensitive yeast secretory mutant sec15-1. Overexpression of ftt1ΔC also suppressed the growth defect of sec2-41, sec3-101, and sec7-1 strains. Overexpression of ftt1ΔC in sec2-41 and sec15-1 strains could also rescue the secretion of invertase at the restrictive temperatures, and overexpression of full-length ftt1 enhanced invertase secretion by wild-type yeast cells. These findings strongly suggest that the T. reesei ftt1 has a role in protein secretion.

Original languageEnglish
Pages (from-to)27-40
Number of pages14
JournalBiochimica et Biophysica Acta: Molecular Cell Research
Volume1590
Issue number1-3
DOIs
Publication statusPublished - 12 Jun 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Secretory Component
14-3-3 Proteins
Trichoderma
Secretory Pathway
beta-Fructofuranosidase
Yeasts
Genes
Temperature
Fungal Proteins
Growth
Proteins
Fungi
Amino Acids
DNA

Keywords

  • 14-3-3
  • Exocytosis
  • Trichoderma reesei/Hypocrea jecorina

Cite this

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title = "Characterisation of two 14-3-3 genes from Trichoderma reesei: Interactions with yeast secretory pathway components",
abstract = "The 14-3-3 proteins are highly conserved, ubiquitously expressed proteins taking part in numerous cellular processes. Two genes encoding 14-3-3 proteins, ftt1 and ftt2, were isolated and characterised from the filamentous fungus Trichoderma reesei. FTTI showed the highest sequence identity (98{\%} at the amino acid level) to the Trichoderma harzianum protein Th1433. FTTII is relatively distinct from FTTI, showing approximately 75{\%} identity to other fungal 14-3-3 proteins. Despite their sequence divergence, both of the T. reesei ftt genes were equally able to complement the yeast bmh1 bmh2 double disruption. The T. reesei ftt genes were also found to be quite closely linked in the genomic DNA. A C-terminally truncated version of ftt1 (ftt1ΔC) was first isolated as a multicopy suppressor of the growth defect of the temperature-sensitive yeast secretory mutant sec15-1. Overexpression of ftt1ΔC also suppressed the growth defect of sec2-41, sec3-101, and sec7-1 strains. Overexpression of ftt1ΔC in sec2-41 and sec15-1 strains could also rescue the secretion of invertase at the restrictive temperatures, and overexpression of full-length ftt1 enhanced invertase secretion by wild-type yeast cells. These findings strongly suggest that the T. reesei ftt1 has a role in protein secretion.",
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Characterisation of two 14-3-3 genes from Trichoderma reesei : Interactions with yeast secretory pathway components. / Vasara, Tuija; Keränen, Sirkka; Penttilä, Merja; Saloheimo, Markku.

In: Biochimica et Biophysica Acta: Molecular Cell Research, Vol. 1590, No. 1-3, 12.06.2002, p. 27-40.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterisation of two 14-3-3 genes from Trichoderma reesei

T2 - Interactions with yeast secretory pathway components

AU - Vasara, Tuija

AU - Keränen, Sirkka

AU - Penttilä, Merja

AU - Saloheimo, Markku

PY - 2002/6/12

Y1 - 2002/6/12

N2 - The 14-3-3 proteins are highly conserved, ubiquitously expressed proteins taking part in numerous cellular processes. Two genes encoding 14-3-3 proteins, ftt1 and ftt2, were isolated and characterised from the filamentous fungus Trichoderma reesei. FTTI showed the highest sequence identity (98% at the amino acid level) to the Trichoderma harzianum protein Th1433. FTTII is relatively distinct from FTTI, showing approximately 75% identity to other fungal 14-3-3 proteins. Despite their sequence divergence, both of the T. reesei ftt genes were equally able to complement the yeast bmh1 bmh2 double disruption. The T. reesei ftt genes were also found to be quite closely linked in the genomic DNA. A C-terminally truncated version of ftt1 (ftt1ΔC) was first isolated as a multicopy suppressor of the growth defect of the temperature-sensitive yeast secretory mutant sec15-1. Overexpression of ftt1ΔC also suppressed the growth defect of sec2-41, sec3-101, and sec7-1 strains. Overexpression of ftt1ΔC in sec2-41 and sec15-1 strains could also rescue the secretion of invertase at the restrictive temperatures, and overexpression of full-length ftt1 enhanced invertase secretion by wild-type yeast cells. These findings strongly suggest that the T. reesei ftt1 has a role in protein secretion.

AB - The 14-3-3 proteins are highly conserved, ubiquitously expressed proteins taking part in numerous cellular processes. Two genes encoding 14-3-3 proteins, ftt1 and ftt2, were isolated and characterised from the filamentous fungus Trichoderma reesei. FTTI showed the highest sequence identity (98% at the amino acid level) to the Trichoderma harzianum protein Th1433. FTTII is relatively distinct from FTTI, showing approximately 75% identity to other fungal 14-3-3 proteins. Despite their sequence divergence, both of the T. reesei ftt genes were equally able to complement the yeast bmh1 bmh2 double disruption. The T. reesei ftt genes were also found to be quite closely linked in the genomic DNA. A C-terminally truncated version of ftt1 (ftt1ΔC) was first isolated as a multicopy suppressor of the growth defect of the temperature-sensitive yeast secretory mutant sec15-1. Overexpression of ftt1ΔC also suppressed the growth defect of sec2-41, sec3-101, and sec7-1 strains. Overexpression of ftt1ΔC in sec2-41 and sec15-1 strains could also rescue the secretion of invertase at the restrictive temperatures, and overexpression of full-length ftt1 enhanced invertase secretion by wild-type yeast cells. These findings strongly suggest that the T. reesei ftt1 has a role in protein secretion.

KW - 14-3-3

KW - Exocytosis

KW - Trichoderma reesei/Hypocrea jecorina

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U2 - 10.1016/S0167-4889(02)00197-0

DO - 10.1016/S0167-4889(02)00197-0

M3 - Article

VL - 1590

SP - 27

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JO - Biochimica et Biophysica Acta: Molecular Cell Research

JF - Biochimica et Biophysica Acta: Molecular Cell Research

SN - 0167-4889

IS - 1-3

ER -