Characterization and applications of a trioctyl(3/4-vinylbenzyl)phosphonium stationary phase for use in capillary liquid chromatography

Dana Moravcová (Corresponding Author), Josef Planeta, Pavel Karásek, Alistair W.T. King, Susanne K. Wiedmer

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The morphology, composition, and selectivity of a silica-based monolithic stationary phase, grafted by a layer of trioctyl(3/4-vinylbenzyl)phosphonium chloride ([P888VBn]Cl), is presented. The results of elemental analysis confirmed that the prepared stationary phase contains 38.8 at.% of silicon, 60.2 at.% of carbon, and 1.0 at.% of phosphorus. Capillary columns (150 × 0.1 mm) for liquid chromatography were evaluated using alkylbenzenes, monosubstituted benzenes, polyaromatic compounds, substituted benzene regioisomers, and aromatic carboxylic and amino acids. The prepared ionic liquid (IL)-based stationary phase exhibits hydrophobic, hydrophilic, and electrostatic interactions, as confirmed by experiments on the evaluation of the effect of the mobile phase composition (content of acetonitrile and ammonium formate) on the isocratic chromatographic separation. Thus, the IL-based capillary column demonstrates a unique separation selectivity compared to Phenyl-, C8-, and C18-stationary phases, and high efficiency for an expanding number of structurally diverse compounds.
Original languageEnglish
Article number462866
JournalJournal of Chromatography A
Volume1666
DOIs
Publication statusPublished - 15 Mar 2022
MoE publication typeA1 Journal article-refereed

Keywords

  • Chromatography
  • Ion liquid
  • Mixed-mode interactions
  • Monolith
  • Polar and nonpolar compounds

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