TY - JOUR
T1 - Characterization and applications of a trioctyl(3/4-vinylbenzyl)phosphonium stationary phase for use in capillary liquid chromatography
AU - Moravcová, Dana
AU - Planeta, Josef
AU - Karásek, Pavel
AU - King, Alistair W.T.
AU - Wiedmer, Susanne K.
N1 - Funding Information:
This contribution has been supported by the Czech Science Foundation (Project No. GA19–00742S) and by the Czech Academy of Sciences (institutional support RVO:68081715).
PY - 2022/3/15
Y1 - 2022/3/15
N2 - The morphology, composition, and selectivity of a silica-based monolithic stationary phase, grafted by a layer of trioctyl(3/4-vinylbenzyl)phosphonium chloride ([P888VBn]Cl), is presented. The results of elemental analysis confirmed that the prepared stationary phase contains 38.8 at.% of silicon, 60.2 at.% of carbon, and 1.0 at.% of phosphorus. Capillary columns (150 × 0.1 mm) for liquid chromatography were evaluated using alkylbenzenes, monosubstituted benzenes, polyaromatic compounds, substituted benzene regioisomers, and aromatic carboxylic and amino acids. The prepared ionic liquid (IL)-based stationary phase exhibits hydrophobic, hydrophilic, and electrostatic interactions, as confirmed by experiments on the evaluation of the effect of the mobile phase composition (content of acetonitrile and ammonium formate) on the isocratic chromatographic separation. Thus, the IL-based capillary column demonstrates a unique separation selectivity compared to Phenyl-, C8-, and C18-stationary phases, and high efficiency for an expanding number of structurally diverse compounds.
AB - The morphology, composition, and selectivity of a silica-based monolithic stationary phase, grafted by a layer of trioctyl(3/4-vinylbenzyl)phosphonium chloride ([P888VBn]Cl), is presented. The results of elemental analysis confirmed that the prepared stationary phase contains 38.8 at.% of silicon, 60.2 at.% of carbon, and 1.0 at.% of phosphorus. Capillary columns (150 × 0.1 mm) for liquid chromatography were evaluated using alkylbenzenes, monosubstituted benzenes, polyaromatic compounds, substituted benzene regioisomers, and aromatic carboxylic and amino acids. The prepared ionic liquid (IL)-based stationary phase exhibits hydrophobic, hydrophilic, and electrostatic interactions, as confirmed by experiments on the evaluation of the effect of the mobile phase composition (content of acetonitrile and ammonium formate) on the isocratic chromatographic separation. Thus, the IL-based capillary column demonstrates a unique separation selectivity compared to Phenyl-, C8-, and C18-stationary phases, and high efficiency for an expanding number of structurally diverse compounds.
KW - Chromatography
KW - Ion liquid
KW - Mixed-mode interactions
KW - Monolith
KW - Polar and nonpolar compounds
UR - http://www.scopus.com/inward/record.url?scp=85124013529&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2022.462866
DO - 10.1016/j.chroma.2022.462866
M3 - Article
VL - 1666
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
M1 - 462866
ER -