Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 229-238 |
| Number of pages | 9 |
| Journal | Electroanalysis |
| Volume | 24 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- PQQ-dependent aldose dehydrogenase
- biofuel cells
- bioanodes
- stability
- enzyme immobilization
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Characterization and stability study of immobilized PQQ-dependent aldose dehydrogenase bioanodes. / Tuurala, Saara; Lau, C.; Atanassov, P.; Smolander, Maria (Corresponding Author); Minteer, S.D. (Corresponding Author).
In: Electroanalysis, Vol. 24, No. 2, 2012, p. 229-238.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Characterization and stability study of immobilized PQQ-dependent aldose dehydrogenase bioanodes
AU - Tuurala, Saara
AU - Lau, C.
AU - Atanassov, P.
AU - Smolander, Maria
AU - Minteer, S.D.
PY - 2012
Y1 - 2012
N2 - In this paper, glucose oxidizing bioanodes employing immobilized PQQ‐dependent aldose dehydrogenase were prepared and characterized. The enzyme was immobilized on carbon paper in two different polymeric systems: tetrabutylammonium bromide (TBAB) modified Nafion and butanal modified chitosan. Characterization of the bioanodes included electron microscopy, electrochemical evaluation, as well as stability and leaching studies. Results indicate that the operational degradation was the same but the long term storage stability is better in the case of modified Nafion. The performance of the modified Nafion immobilized bioanodes stayed at 70 % of the initial value after 60 days of storing at 4 °C and 25 °C. Compared to TBAB modified Nafion immobilized bioanodes, butanal modified chitosan immobilized bioanodes showed 50 % activity after eight weeks storage at 4°C and one week storage at 25 °C. However, the electrochemical properties of modified chitosan were better.
AB - In this paper, glucose oxidizing bioanodes employing immobilized PQQ‐dependent aldose dehydrogenase were prepared and characterized. The enzyme was immobilized on carbon paper in two different polymeric systems: tetrabutylammonium bromide (TBAB) modified Nafion and butanal modified chitosan. Characterization of the bioanodes included electron microscopy, electrochemical evaluation, as well as stability and leaching studies. Results indicate that the operational degradation was the same but the long term storage stability is better in the case of modified Nafion. The performance of the modified Nafion immobilized bioanodes stayed at 70 % of the initial value after 60 days of storing at 4 °C and 25 °C. Compared to TBAB modified Nafion immobilized bioanodes, butanal modified chitosan immobilized bioanodes showed 50 % activity after eight weeks storage at 4°C and one week storage at 25 °C. However, the electrochemical properties of modified chitosan were better.
KW - PQQ-dependent aldose dehydrogenase
KW - biofuel cells
KW - bioanodes
KW - stability
KW - enzyme immobilization
U2 - 10.1002/elan.201100546
DO - 10.1002/elan.201100546
M3 - Article
VL - 24
SP - 229
EP - 238
JO - Electroanalysis
JF - Electroanalysis
SN - 1040-0397
IS - 2
ER -