Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8

Riitta Puupponen-Pimiä; Markku Saloheimo; Tuija Vasara; Runar Ra; Janka Gaugecz; Ulrika Kurten; Jonathan Knowles; Sirkka Keränen; Veli Kauppinen

doi:10.1007/BF00029018

Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8

Riitta Puupponen-Pimiä, Markku Saloheimo, Tuija Vasara, Runar Ra, Janka Gaugecz, Ulrika Kurten, Jonathan Knowles, Sirkka Keränen, Veli Kauppinen

Research output: Contribution to journal › Article › Scientific › peer-review

17 Citations (Scopus)

Abstract

We have isolated the birch homologue (BP8) for the carrot embryogenic gene DC8 by heterologous hybridization. The birch BP8 gene encodes a putative protein of 53 kDa, showing 52% sequence identity with the DC8 gene at the amino acid level. The putative BP8 protein contains 20 repeats of 11 amino acids and thus belongs to the group of LEA proteins isolated from such plants as carrot, cotton and wheat. Northern hybridization of mRNA isolated from birch cells representing different stages of somatic embryogenesis and non-embryogenetic material with a PB8 probe gave no signals, suggesting a low expression level of the BP8 gene.

Original language	English
Pages (from-to)	423 - 428
Number of pages	6
Journal	Plant Molecular Biology
Volume	23
Issue number	2
DOIs	https://doi.org/10.1007/BF00029018
Publication status	Published - 1993
MoE publication type	A1 Journal article-refereed

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10.1007/BF00029018

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title = "Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8",

abstract = "We have isolated the birch homologue (BP8) for the carrot embryogenic gene DC8 by heterologous hybridization. The birch BP8 gene encodes a putative protein of 53 kDa, showing 52% sequence identity with the DC8 gene at the amino acid level. The putative BP8 protein contains 20 repeats of 11 amino acids and thus belongs to the group of LEA proteins isolated from such plants as carrot, cotton and wheat. Northern hybridization of mRNA isolated from birch cells representing different stages of somatic embryogenesis and non-embryogenetic material with a PB8 probe gave no signals, suggesting a low expression level of the BP8 gene.",

author = "Riitta Puupponen-Pimi{\"a} and Markku Saloheimo and Tuija Vasara and Runar Ra and Janka Gaugecz and Ulrika Kurten and Jonathan Knowles and Sirkka Ker{\"a}nen and Veli Kauppinen",

note = "Project code: BIO9001",

year = "1993",

doi = "10.1007/BF00029018",

language = "English",

volume = "23",

pages = "423 -- 428",

journal = "Plant Molecular Biology",

issn = "0167-4412",

publisher = "Springer Science and Business Media B.V.",

number = "2",

}

TY - JOUR

T1 - Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8

AU - Puupponen-Pimiä, Riitta

AU - Saloheimo, Markku

AU - Vasara, Tuija

AU - Ra, Runar

AU - Gaugecz, Janka

AU - Kurten, Ulrika

AU - Knowles, Jonathan

AU - Keränen, Sirkka

AU - Kauppinen, Veli

N1 - Project code: BIO9001

PY - 1993

Y1 - 1993

N2 - We have isolated the birch homologue (BP8) for the carrot embryogenic gene DC8 by heterologous hybridization. The birch BP8 gene encodes a putative protein of 53 kDa, showing 52% sequence identity with the DC8 gene at the amino acid level. The putative BP8 protein contains 20 repeats of 11 amino acids and thus belongs to the group of LEA proteins isolated from such plants as carrot, cotton and wheat. Northern hybridization of mRNA isolated from birch cells representing different stages of somatic embryogenesis and non-embryogenetic material with a PB8 probe gave no signals, suggesting a low expression level of the BP8 gene.

AB - We have isolated the birch homologue (BP8) for the carrot embryogenic gene DC8 by heterologous hybridization. The birch BP8 gene encodes a putative protein of 53 kDa, showing 52% sequence identity with the DC8 gene at the amino acid level. The putative BP8 protein contains 20 repeats of 11 amino acids and thus belongs to the group of LEA proteins isolated from such plants as carrot, cotton and wheat. Northern hybridization of mRNA isolated from birch cells representing different stages of somatic embryogenesis and non-embryogenetic material with a PB8 probe gave no signals, suggesting a low expression level of the BP8 gene.

U2 - 10.1007/BF00029018

DO - 10.1007/BF00029018

M3 - Article

SN - 0167-4412

VL - 23

SP - 423

EP - 428

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 2

ER -

Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8

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