Characterization of a birch (Betula pendula Roth.) embryogenic gene, BP8

Riitta Puupponen-Pimiä, Markku Saloheimo, Tuija Vasara, Runar Ra, Janka Gaugecz, Ulrika Kurten, Jonathan Knowles, Sirkka Keränen, Veli Kauppinen

    Research output: Contribution to journalArticleScientificpeer-review

    17 Citations (Scopus)

    Abstract

    We have isolated the birch homologue (BP8) for the carrot embryogenic gene DC8 by heterologous hybridization. The birch BP8 gene encodes a putative protein of 53 kDa, showing 52% sequence identity with the DC8 gene at the amino acid level. The putative BP8 protein contains 20 repeats of 11 amino acids and thus belongs to the group of LEA proteins isolated from such plants as carrot, cotton and wheat. Northern hybridization of mRNA isolated from birch cells representing different stages of somatic embryogenesis and non-embryogenetic material with a PB8 probe gave no signals, suggesting a low expression level of the BP8 gene.

    Original languageEnglish
    Pages (from-to)423 - 428
    Number of pages6
    JournalPlant Molecular Biology
    Volume23
    Issue number2
    DOIs
    Publication statusPublished - 1993
    MoE publication typeA1 Journal article-refereed

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