Characterization of a double-sided silicon strip detector autoradiography system

Anders Örbom, Jonas Ahlstedt, Tom Serén, Iiro Auterinen, Petri Kotiluoto, Håvard Hauge, Karl Östlund, Tove Olafsen, Anna M. Wu, Magnus Dahlbom, Sven Erik Strand

    Research output: Contribution to journalArticleScientificpeer-review

    2 Citations (Scopus)

    Abstract

    Purpose: The most commonly used technology currently used for autoradiography is storage phosphor screens, which has many benefits such as a large field of view but lacks particle-counting detection of the time and energy of each detected radionuclide decay. A number of alternative designs, using either solid state or scintillator detectors, have been developed to address these issues. The aim of this study is to characterize the imaging performance of one such instrument, a double-sided silicon strip detector (DSSD) system for digital autoradiography. A novel aspect of this work is that the instrument, in contrast to previous prototype systems using the same detector type, provides the ability for user accessible imaging with higher throughput. Studies were performed to compare its spatial resolution to that of storage phosphor screens and test the implementation of multiradionuclide ex vivo imaging in a mouse preclinical animal study. Methods: Detector background counts were determined by measuring a nonradioactive sample slide for 52 h. Energy spectra and detection efficiency were measured for seven commonly used radionuclides under representative conditions for tissue imaging. System dead time was measured by imaging 18F samples of at least 5 kBq and studying the changes in count rate over time. A line source of 58Co was manufactured by irradiating a 10 ?m nickel wire with fast neutrons in a research reactor. Samples of this wire were imaged in both the DSSD and storage phosphor screen systems and the full width at half maximum (FWHM) measured for the line profiles. Multiradionuclide imaging was employed in a two animal study to examine the intratumoral distribution of a 125I-labeled monoclonal antibody and a 131I-labeled engineered fragment (diabody) injected in the same mouse, both targeting carcinoembryonic antigen. Results: Detector background was 1.81×10?6 counts per second per 50×50 ?mpixel. Energy spectra and detection efficiency were successfully measured for seven radionuclides. The system dead time was measured to be 59 ?s, and FWHM for a 58Co line source was 154±14 ?m for the DSSD system and 343±15 ?m for the storage phosphor system. Separation of the contributions from 125I and 131I was performed on autoradiography images of tumor sections. Conclusions: This study has shown that a DSSD system can be beneficially applied for digital autoradiography with simultaneous multiradionuclide imaging capability. The system has a low background signal, ability to image both low and high activity samples, and a good energy resolution.

    Original languageEnglish
    Article number575
    Pages (from-to)575-584
    Number of pages10
    JournalMedical Physics
    Volume42
    Issue number2
    DOIs
    Publication statusPublished - 1 Feb 2015
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Silicon
    Autoradiography
    Radioisotopes
    Fast Neutrons
    Carcinoembryonic Antigen
    Nickel
    Monoclonal Antibodies
    Technology
    Research
    Neoplasms

    Keywords

    • autoradiography
    • molecular imaging
    • silicon-strip detectors
    • solid-state detectors

    Cite this

    Örbom, Anders ; Ahlstedt, Jonas ; Serén, Tom ; Auterinen, Iiro ; Kotiluoto, Petri ; Hauge, Håvard ; Östlund, Karl ; Olafsen, Tove ; Wu, Anna M. ; Dahlbom, Magnus ; Strand, Sven Erik. / Characterization of a double-sided silicon strip detector autoradiography system. In: Medical Physics. 2015 ; Vol. 42, No. 2. pp. 575-584.
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    title = "Characterization of a double-sided silicon strip detector autoradiography system",
    abstract = "Purpose: The most commonly used technology currently used for autoradiography is storage phosphor screens, which has many benefits such as a large field of view but lacks particle-counting detection of the time and energy of each detected radionuclide decay. A number of alternative designs, using either solid state or scintillator detectors, have been developed to address these issues. The aim of this study is to characterize the imaging performance of one such instrument, a double-sided silicon strip detector (DSSD) system for digital autoradiography. A novel aspect of this work is that the instrument, in contrast to previous prototype systems using the same detector type, provides the ability for user accessible imaging with higher throughput. Studies were performed to compare its spatial resolution to that of storage phosphor screens and test the implementation of multiradionuclide ex vivo imaging in a mouse preclinical animal study. Methods: Detector background counts were determined by measuring a nonradioactive sample slide for 52 h. Energy spectra and detection efficiency were measured for seven commonly used radionuclides under representative conditions for tissue imaging. System dead time was measured by imaging 18F samples of at least 5 kBq and studying the changes in count rate over time. A line source of 58Co was manufactured by irradiating a 10 ?m nickel wire with fast neutrons in a research reactor. Samples of this wire were imaged in both the DSSD and storage phosphor screen systems and the full width at half maximum (FWHM) measured for the line profiles. Multiradionuclide imaging was employed in a two animal study to examine the intratumoral distribution of a 125I-labeled monoclonal antibody and a 131I-labeled engineered fragment (diabody) injected in the same mouse, both targeting carcinoembryonic antigen. Results: Detector background was 1.81×10?6 counts per second per 50×50 ?mpixel. Energy spectra and detection efficiency were successfully measured for seven radionuclides. The system dead time was measured to be 59 ?s, and FWHM for a 58Co line source was 154±14 ?m for the DSSD system and 343±15 ?m for the storage phosphor system. Separation of the contributions from 125I and 131I was performed on autoradiography images of tumor sections. Conclusions: This study has shown that a DSSD system can be beneficially applied for digital autoradiography with simultaneous multiradionuclide imaging capability. The system has a low background signal, ability to image both low and high activity samples, and a good energy resolution.",
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    author = "Anders {\"O}rbom and Jonas Ahlstedt and Tom Ser{\'e}n and Iiro Auterinen and Petri Kotiluoto and H{\aa}vard Hauge and Karl {\"O}stlund and Tove Olafsen and Wu, {Anna M.} and Magnus Dahlbom and Strand, {Sven Erik}",
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    Örbom, A, Ahlstedt, J, Serén, T, Auterinen, I, Kotiluoto, P, Hauge, H, Östlund, K, Olafsen, T, Wu, AM, Dahlbom, M & Strand, SE 2015, 'Characterization of a double-sided silicon strip detector autoradiography system', Medical Physics, vol. 42, no. 2, 575, pp. 575-584. https://doi.org/10.1118/1.4905049

    Characterization of a double-sided silicon strip detector autoradiography system. / Örbom, Anders; Ahlstedt, Jonas; Serén, Tom; Auterinen, Iiro; Kotiluoto, Petri; Hauge, Håvard; Östlund, Karl; Olafsen, Tove; Wu, Anna M.; Dahlbom, Magnus; Strand, Sven Erik.

    In: Medical Physics, Vol. 42, No. 2, 575, 01.02.2015, p. 575-584.

    Research output: Contribution to journalArticleScientificpeer-review

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    T1 - Characterization of a double-sided silicon strip detector autoradiography system

    AU - Örbom, Anders

    AU - Ahlstedt, Jonas

    AU - Serén, Tom

    AU - Auterinen, Iiro

    AU - Kotiluoto, Petri

    AU - Hauge, Håvard

    AU - Östlund, Karl

    AU - Olafsen, Tove

    AU - Wu, Anna M.

    AU - Dahlbom, Magnus

    AU - Strand, Sven Erik

    PY - 2015/2/1

    Y1 - 2015/2/1

    N2 - Purpose: The most commonly used technology currently used for autoradiography is storage phosphor screens, which has many benefits such as a large field of view but lacks particle-counting detection of the time and energy of each detected radionuclide decay. A number of alternative designs, using either solid state or scintillator detectors, have been developed to address these issues. The aim of this study is to characterize the imaging performance of one such instrument, a double-sided silicon strip detector (DSSD) system for digital autoradiography. A novel aspect of this work is that the instrument, in contrast to previous prototype systems using the same detector type, provides the ability for user accessible imaging with higher throughput. Studies were performed to compare its spatial resolution to that of storage phosphor screens and test the implementation of multiradionuclide ex vivo imaging in a mouse preclinical animal study. Methods: Detector background counts were determined by measuring a nonradioactive sample slide for 52 h. Energy spectra and detection efficiency were measured for seven commonly used radionuclides under representative conditions for tissue imaging. System dead time was measured by imaging 18F samples of at least 5 kBq and studying the changes in count rate over time. A line source of 58Co was manufactured by irradiating a 10 ?m nickel wire with fast neutrons in a research reactor. Samples of this wire were imaged in both the DSSD and storage phosphor screen systems and the full width at half maximum (FWHM) measured for the line profiles. Multiradionuclide imaging was employed in a two animal study to examine the intratumoral distribution of a 125I-labeled monoclonal antibody and a 131I-labeled engineered fragment (diabody) injected in the same mouse, both targeting carcinoembryonic antigen. Results: Detector background was 1.81×10?6 counts per second per 50×50 ?mpixel. Energy spectra and detection efficiency were successfully measured for seven radionuclides. The system dead time was measured to be 59 ?s, and FWHM for a 58Co line source was 154±14 ?m for the DSSD system and 343±15 ?m for the storage phosphor system. Separation of the contributions from 125I and 131I was performed on autoradiography images of tumor sections. Conclusions: This study has shown that a DSSD system can be beneficially applied for digital autoradiography with simultaneous multiradionuclide imaging capability. The system has a low background signal, ability to image both low and high activity samples, and a good energy resolution.

    AB - Purpose: The most commonly used technology currently used for autoradiography is storage phosphor screens, which has many benefits such as a large field of view but lacks particle-counting detection of the time and energy of each detected radionuclide decay. A number of alternative designs, using either solid state or scintillator detectors, have been developed to address these issues. The aim of this study is to characterize the imaging performance of one such instrument, a double-sided silicon strip detector (DSSD) system for digital autoradiography. A novel aspect of this work is that the instrument, in contrast to previous prototype systems using the same detector type, provides the ability for user accessible imaging with higher throughput. Studies were performed to compare its spatial resolution to that of storage phosphor screens and test the implementation of multiradionuclide ex vivo imaging in a mouse preclinical animal study. Methods: Detector background counts were determined by measuring a nonradioactive sample slide for 52 h. Energy spectra and detection efficiency were measured for seven commonly used radionuclides under representative conditions for tissue imaging. System dead time was measured by imaging 18F samples of at least 5 kBq and studying the changes in count rate over time. A line source of 58Co was manufactured by irradiating a 10 ?m nickel wire with fast neutrons in a research reactor. Samples of this wire were imaged in both the DSSD and storage phosphor screen systems and the full width at half maximum (FWHM) measured for the line profiles. Multiradionuclide imaging was employed in a two animal study to examine the intratumoral distribution of a 125I-labeled monoclonal antibody and a 131I-labeled engineered fragment (diabody) injected in the same mouse, both targeting carcinoembryonic antigen. Results: Detector background was 1.81×10?6 counts per second per 50×50 ?mpixel. Energy spectra and detection efficiency were successfully measured for seven radionuclides. The system dead time was measured to be 59 ?s, and FWHM for a 58Co line source was 154±14 ?m for the DSSD system and 343±15 ?m for the storage phosphor system. Separation of the contributions from 125I and 131I was performed on autoradiography images of tumor sections. Conclusions: This study has shown that a DSSD system can be beneficially applied for digital autoradiography with simultaneous multiradionuclide imaging capability. The system has a low background signal, ability to image both low and high activity samples, and a good energy resolution.

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