Characterization of a feruloyl esterase from Aspergillus terreus facilitates the division of fungal enzymes from Carbohydrate Esterase family 1 of the carbohydrate-active enzymes (CAZy) database

Miia R Mäkelä, Adiphol Dilokpimol, Salla M Koskela, Jaana Kuuskeri, Ronald P de Vries, Kristiina Hildén

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Feruloyl esterases (FAEs) are accessory enzymes for plant biomass degradation, which catalyse hydrolysis of carboxylic ester linkages between hydroxycinnamic acids and plant cell-wall carbohydrates. They are a diverse group of enzymes evolved from, e.g. acetyl xylan esterases (AXEs), lipases and tannases, thus complicating their classification and prediction of function by sequence similarity. Recently, an increasing number of fungal FAEs have been biochemically characterized, owing to their potential in various biotechnological applications and multitude of candidate FAEs in fungal genomes. However, only part of the fungal FAEs are included in Carbohydrate Esterase family 1 (CE1) of the carbohydrate-active enzymes (CAZy) database. In this work, we performed a phylogenetic analysis that divided the fungal members of CE1 into five subfamilies of which three contained characterized enzymes with conserved activities. Conservation within one of the subfamilies was confirmed by characterization of an additional CE1 enzyme from Aspergillus terreus. Recombinant A. terreus FaeD (AtFaeD) showed broad specificity towards synthetic methyl and ethyl esters, and released ferulic acid from plant biomass substrates, demonstrating its true FAE activity and interesting features as potential biocatalyst. The subfamily division of the fungal CE1 members enables more efficient selection of candidate enzymes for biotechnological processes.

Original languageEnglish
Pages (from-to)869-880
Number of pages12
JournalMicrobial Biotechnology
Volume11
Issue number5
DOIs
Publication statusPublished - Sep 2018
MoE publication typeA1 Journal article-refereed

Keywords

  • Aspergillus/enzymology
  • Biomass
  • Biotransformation
  • Carboxylic Ester Hydrolases/classification
  • Cloning, Molecular
  • Coumaric Acids/metabolism
  • Gene Expression
  • Phylogeny
  • Recombinant Proteins/genetics
  • Sequence Homology
  • Substrate Specificity

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