Characterization of a stem cell population in lung cancer A549 cells

Ji-Min Sung; Hee-Jung Cho; Hee Yi; Chi-Ho Lee; Hye-Sun Kim; Dong-Ku Kim; AM Adb El-Aty; Jin-Suk Kim; Christopher P.  Landowski; Matthias Hediger; Ho-Chul Shin

doi:10.1016/j.bbrc.2008.04.038

Characterization of a stem cell population in lung cancer A549 cells

Ji-Min Sung, Hee-Jung Cho, Hee Yi, Chi-Ho Lee, Hye-Sun Kim, Dong-Ku Kim, AM Adb El-Aty, Jin-Suk Kim, Christopher P. Landowski, Matthias Hediger, Ho-Chul Shin (Corresponding Author)

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Abstract

We isolated a stem cell subpopulation from human lung cancer A549 cells using FACS/Hoechst 33342. This side population (SP), which comprised 24% of the total cell population, totally disappeared after treatment with the selective ABCG 2 inhibitor fumitremorgin C. In a repopulation study, isolated SP and non-SP cells were each able to generate a heterogeneous population of SP and non-SP cells, but this repopulation occurred more rapidly in SP cells than non-SP. An MTT assay and cell cycle distribution analysis reveal a similar profile between SP and non-SP groups. However, in the presence of doxorubicin (DOX) and methotrexate (MTX), SP cells showed significantly lower Annexin V staining when compared to non-SP cells. Taken together, these results demonstrate that SP cells have an active regeneration capacity and high anti-apoptotic activity compared with non-SP cells. Furthermore, our GeneChip data revealed a heightened mRNA expression of ABCG2 and ABCC2 in SP cells. Overall these data explain why the SP of A549 has a unique ability to resist DOX and MTX treatments. Therefore, we suggest that the expression of the ABCG2 transporter plays an important role in the multidrug resistance phenotype of A549 SP cells.

Original language	English
Pages (from-to)	163-167
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	371
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2008.04.038
Publication status	Published - 20 Jun 2008
MoE publication type	A1 Journal article-refereed

Keywords

ATP Binding Cassette Transporter, Subfamily G, Member 2
ATP-Binding Cassette Transporters/genetics
Antineoplastic Agents/pharmacology
Apoptosis
Cell Cycle/drug effects
Cell Line, Tumor
Cell Proliferation/drug effects
Doxorubicin/pharmacology
Drug Resistance, Multiple
Drug Resistance, Neoplasm
Gene Expression
Humans
Lung Neoplasms/metabolism
Membrane Transport Proteins/genetics
Methotrexate/pharmacology
Multidrug Resistance-Associated Proteins/genetics
Neoplasm Proteins/genetics
Neoplastic Stem Cells/drug effects

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2008.04.038

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@article{6945770c64c240a4a3891f136628561d,

title = "Characterization of a stem cell population in lung cancer A549 cells",

abstract = "We isolated a stem cell subpopulation from human lung cancer A549 cells using FACS/Hoechst 33342. This side population (SP), which comprised 24% of the total cell population, totally disappeared after treatment with the selective ABCG 2 inhibitor fumitremorgin C. In a repopulation study, isolated SP and non-SP cells were each able to generate a heterogeneous population of SP and non-SP cells, but this repopulation occurred more rapidly in SP cells than non-SP. An MTT assay and cell cycle distribution analysis reveal a similar profile between SP and non-SP groups. However, in the presence of doxorubicin (DOX) and methotrexate (MTX), SP cells showed significantly lower Annexin V staining when compared to non-SP cells. Taken together, these results demonstrate that SP cells have an active regeneration capacity and high anti-apoptotic activity compared with non-SP cells. Furthermore, our GeneChip data revealed a heightened mRNA expression of ABCG2 and ABCC2 in SP cells. Overall these data explain why the SP of A549 has a unique ability to resist DOX and MTX treatments. Therefore, we suggest that the expression of the ABCG2 transporter plays an important role in the multidrug resistance phenotype of A549 SP cells.",

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author = "Ji-Min Sung and Hee-Jung Cho and Hee Yi and Chi-Ho Lee and Hye-Sun Kim and Dong-Ku Kim and {Adb El-Aty}, AM and Jin-Suk Kim and Landowski, {Christopher P.} and Matthias Hediger and Ho-Chul Shin",

year = "2008",

month = jun,

day = "20",

doi = "10.1016/j.bbrc.2008.04.038",

language = "English",

volume = "371",

pages = "163--167",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Characterization of a stem cell population in lung cancer A549 cells

AU - Sung, Ji-Min

AU - Cho, Hee-Jung

AU - Yi, Hee

AU - Lee, Chi-Ho

AU - Kim, Hye-Sun

AU - Kim, Dong-Ku

AU - Adb El-Aty, AM

AU - Kim, Jin-Suk

AU - Landowski, Christopher P.

AU - Hediger, Matthias

AU - Shin, Ho-Chul

PY - 2008/6/20

Y1 - 2008/6/20

N2 - We isolated a stem cell subpopulation from human lung cancer A549 cells using FACS/Hoechst 33342. This side population (SP), which comprised 24% of the total cell population, totally disappeared after treatment with the selective ABCG 2 inhibitor fumitremorgin C. In a repopulation study, isolated SP and non-SP cells were each able to generate a heterogeneous population of SP and non-SP cells, but this repopulation occurred more rapidly in SP cells than non-SP. An MTT assay and cell cycle distribution analysis reveal a similar profile between SP and non-SP groups. However, in the presence of doxorubicin (DOX) and methotrexate (MTX), SP cells showed significantly lower Annexin V staining when compared to non-SP cells. Taken together, these results demonstrate that SP cells have an active regeneration capacity and high anti-apoptotic activity compared with non-SP cells. Furthermore, our GeneChip data revealed a heightened mRNA expression of ABCG2 and ABCC2 in SP cells. Overall these data explain why the SP of A549 has a unique ability to resist DOX and MTX treatments. Therefore, we suggest that the expression of the ABCG2 transporter plays an important role in the multidrug resistance phenotype of A549 SP cells.

AB - We isolated a stem cell subpopulation from human lung cancer A549 cells using FACS/Hoechst 33342. This side population (SP), which comprised 24% of the total cell population, totally disappeared after treatment with the selective ABCG 2 inhibitor fumitremorgin C. In a repopulation study, isolated SP and non-SP cells were each able to generate a heterogeneous population of SP and non-SP cells, but this repopulation occurred more rapidly in SP cells than non-SP. An MTT assay and cell cycle distribution analysis reveal a similar profile between SP and non-SP groups. However, in the presence of doxorubicin (DOX) and methotrexate (MTX), SP cells showed significantly lower Annexin V staining when compared to non-SP cells. Taken together, these results demonstrate that SP cells have an active regeneration capacity and high anti-apoptotic activity compared with non-SP cells. Furthermore, our GeneChip data revealed a heightened mRNA expression of ABCG2 and ABCC2 in SP cells. Overall these data explain why the SP of A549 has a unique ability to resist DOX and MTX treatments. Therefore, we suggest that the expression of the ABCG2 transporter plays an important role in the multidrug resistance phenotype of A549 SP cells.

KW - ATP Binding Cassette Transporter, Subfamily G, Member 2

KW - ATP-Binding Cassette Transporters/genetics

KW - Antineoplastic Agents/pharmacology

KW - Apoptosis

KW - Cell Cycle/drug effects

KW - Cell Line, Tumor

KW - Cell Proliferation/drug effects

KW - Doxorubicin/pharmacology

KW - Drug Resistance, Multiple

KW - Drug Resistance, Neoplasm

KW - Gene Expression

KW - Humans

KW - Lung Neoplasms/metabolism

KW - Membrane Transport Proteins/genetics

KW - Methotrexate/pharmacology

KW - Multidrug Resistance-Associated Proteins/genetics

KW - Neoplasm Proteins/genetics

KW - Neoplastic Stem Cells/drug effects

U2 - 10.1016/j.bbrc.2008.04.038

DO - 10.1016/j.bbrc.2008.04.038

M3 - Article

C2 - 18423378

SN - 0006-291X

VL - 371

SP - 163

EP - 167

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -

Characterization of a stem cell population in lung cancer A549 cells

Abstract

Keywords

UN SDGs

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