Characterization of aerobic bacterial and fungal microbiota on surfaces of historic Scottish monuments

Maija-Liisa Suihko (Corresponding Author), Hanna-Leena Alakomi, Anna Gorbushina, Irene Fortune, Jürgen Marquardt, Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

50 Citations (Scopus)

Abstract

Twenty samples were taken from the inner or outer surfaces of stone monuments of six historic Scottish buildings and ruins. Biofilms developing on mineral substrates were analysed by in situ scanning electron microscopy and cultivation. Various methods were used to characterize the isolates including automated ribotyping, RAPD and sequencing of the 16S rRNA gene for bacteria, and stereomicroscopy and sequencing of the Internal Transcribed Spacers (ITS) for fungi. Most samples contained microbes between 105 and 107 cfu g−1 substrate. Actinobacteria belonging to the genus Streptomyces (17 samples/5 monuments) or Arthrobacter (12/3) and Pseudomonas (9/3) were frequently detected. Most streptomycetes were in terms of their 16S rRNA gene sequence most closely related to S. microflavus (10/3) or to the undescribed species S. “vulgaris” (8/3). Indoor and outdoor biofilms exhibited significant differences in their microbiota, as shown by both microscopy and isolation studies. Pigmented coccoid Arthrobacter species were typical for the outdoor samples, whereas Pseudomonas species were common in the indoor samples. Based on the low phylogenetic relationship to a known species (type strain), potential novel pigmented bacterial species belonging to the genera Arthrobacter, Brevundimonas, Cryseobacterium, Deinococcus and Dyadobacter were detected from the outdoor samples and to Pseudomonas from the indoor samples. Hyaline fungal species of Acremonium (10/4) mainly occurred in indoor samples, whereas pigmented species of Cladosporium (8/3), Penicillium (6/3) and Phialophora (6/2) were found outdoors. Using in situ microscopy diatom algae were also detected.
Original languageEnglish
Pages (from-to)494-508
JournalSystematic and Applied Microbiology
Volume30
Issue number6
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Keywords

  • Historic monuments
  • biofilm
  • Actinobacteria
  • Streptomyces
  • Arthrobacter
  • Fungi
  • Cladosporium
  • Phialophora
  • Protective pigmentation

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