Abstract
Cellulase and hemicellulase enzymes were produced by Trichoderma reesei RUT C30 on steam pretreated spruce, willow, corn stover and delignified lignocellulose (Solka Floc), as a reference. The enzymes produced were characterized by protein and various enzyme activity measurements. On steam pretreated corn stover higher cellulolytic enzyme activities were reached than on Solka Floc, while the activities obtained on steam pretreated spruce and willow were considerably lower. The produced and two commercial cellulases (Celluclast 1.5 L and Econase CE) were compared by determining specific activities. There were minor differences among the enzymes corresponding to their specific cellulase activities. In contrast, within hemicellulase and β-glucosidase activities, the differences were found to be more significant. It should be also noted that commercial cellulases had considerably higher specific acetyl xylan esterase activities than the produced enzymes. According to subsequent hydrolysis experiments, performed to characterize the produced enzyme complexes to evaluate their applicability for hydrolysis and enzyme production, it seems that the application of the enzyme that was produced on the same substrate as was used for hydrolysis can be advantageous in the case of some substrates. As a result, these experiments demonstrated that pretreated corn stover is a good substrate both for enzyme production and hydrolysis, since high cellulolytic activities could be reached using it as carbon source. Moreover, high sugar yields could be obtained in the hydrolysis by the enzyme produced on steam pretreated corn stover.
| Original language | English |
|---|---|
| Pages (from-to) | 3519 - 3525 |
| Number of pages | 7 |
| Journal | Process Biochemistry |
| Volume | 40 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2005 |
| MoE publication type | A1 Journal article-refereed |
Fingerprint
Keywords
- acetyl xylan esterase
- alpha-Arabinosidase
- corn stover
- Endoglucanase
- alpha-Galactosidase
- alpha-Glucosidase
- Hypocrea jecorina
- Mannanase
- alpha-Xylosidase
- Cellulase
- Hemicellulase
- Trichoderma reesei
- Xylanase
Cite this
}
Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources. / Juhasz, T. (Corresponding Author); Szengyel, Z.; Reczey, K.; Siika-aho, Matti; Viikari, Liisa.
In: Process Biochemistry, Vol. 40, No. 11, 2005, p. 3519 - 3525.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources
AU - Juhasz, T.
AU - Szengyel, Z.
AU - Reczey, K.
AU - Siika-aho, Matti
AU - Viikari, Liisa
PY - 2005
Y1 - 2005
N2 - Cellulase and hemicellulase enzymes were produced by Trichoderma reesei RUT C30 on steam pretreated spruce, willow, corn stover and delignified lignocellulose (Solka Floc), as a reference. The enzymes produced were characterized by protein and various enzyme activity measurements. On steam pretreated corn stover higher cellulolytic enzyme activities were reached than on Solka Floc, while the activities obtained on steam pretreated spruce and willow were considerably lower. The produced and two commercial cellulases (Celluclast 1.5 L and Econase CE) were compared by determining specific activities. There were minor differences among the enzymes corresponding to their specific cellulase activities. In contrast, within hemicellulase and β-glucosidase activities, the differences were found to be more significant. It should be also noted that commercial cellulases had considerably higher specific acetyl xylan esterase activities than the produced enzymes. According to subsequent hydrolysis experiments, performed to characterize the produced enzyme complexes to evaluate their applicability for hydrolysis and enzyme production, it seems that the application of the enzyme that was produced on the same substrate as was used for hydrolysis can be advantageous in the case of some substrates. As a result, these experiments demonstrated that pretreated corn stover is a good substrate both for enzyme production and hydrolysis, since high cellulolytic activities could be reached using it as carbon source. Moreover, high sugar yields could be obtained in the hydrolysis by the enzyme produced on steam pretreated corn stover.
AB - Cellulase and hemicellulase enzymes were produced by Trichoderma reesei RUT C30 on steam pretreated spruce, willow, corn stover and delignified lignocellulose (Solka Floc), as a reference. The enzymes produced were characterized by protein and various enzyme activity measurements. On steam pretreated corn stover higher cellulolytic enzyme activities were reached than on Solka Floc, while the activities obtained on steam pretreated spruce and willow were considerably lower. The produced and two commercial cellulases (Celluclast 1.5 L and Econase CE) were compared by determining specific activities. There were minor differences among the enzymes corresponding to their specific cellulase activities. In contrast, within hemicellulase and β-glucosidase activities, the differences were found to be more significant. It should be also noted that commercial cellulases had considerably higher specific acetyl xylan esterase activities than the produced enzymes. According to subsequent hydrolysis experiments, performed to characterize the produced enzyme complexes to evaluate their applicability for hydrolysis and enzyme production, it seems that the application of the enzyme that was produced on the same substrate as was used for hydrolysis can be advantageous in the case of some substrates. As a result, these experiments demonstrated that pretreated corn stover is a good substrate both for enzyme production and hydrolysis, since high cellulolytic activities could be reached using it as carbon source. Moreover, high sugar yields could be obtained in the hydrolysis by the enzyme produced on steam pretreated corn stover.
KW - acetyl xylan esterase
KW - alpha-Arabinosidase
KW - corn stover
KW - Endoglucanase
KW - alpha-Galactosidase
KW - alpha-Glucosidase
KW - Hypocrea jecorina
KW - Mannanase
KW - alpha-Xylosidase
KW - Cellulase
KW - Hemicellulase
KW - Trichoderma reesei
KW - Xylanase
U2 - 10.1016/j.procbio.2005.03.057
DO - 10.1016/j.procbio.2005.03.057
M3 - Article
VL - 40
SP - 3519
EP - 3525
JO - Process Biochemistry
JF - Process Biochemistry
SN - 1359-5113
IS - 11
ER -