Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources

T. Juhasz (Corresponding Author), Z. Szengyel, K. Reczey, Matti Siika-aho, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

192 Citations (Scopus)

Abstract

Cellulase and hemicellulase enzymes were produced by Trichoderma reesei RUT C30 on steam pretreated spruce, willow, corn stover and delignified lignocellulose (Solka Floc), as a reference. The enzymes produced were characterized by protein and various enzyme activity measurements. On steam pretreated corn stover higher cellulolytic enzyme activities were reached than on Solka Floc, while the activities obtained on steam pretreated spruce and willow were considerably lower. The produced and two commercial cellulases (Celluclast 1.5 L and Econase CE) were compared by determining specific activities. There were minor differences among the enzymes corresponding to their specific cellulase activities. In contrast, within hemicellulase and β-glucosidase activities, the differences were found to be more significant. It should be also noted that commercial cellulases had considerably higher specific acetyl xylan esterase activities than the produced enzymes. According to subsequent hydrolysis experiments, performed to characterize the produced enzyme complexes to evaluate their applicability for hydrolysis and enzyme production, it seems that the application of the enzyme that was produced on the same substrate as was used for hydrolysis can be advantageous in the case of some substrates. As a result, these experiments demonstrated that pretreated corn stover is a good substrate both for enzyme production and hydrolysis, since high cellulolytic activities could be reached using it as carbon source. Moreover, high sugar yields could be obtained in the hydrolysis by the enzyme produced on steam pretreated corn stover.

Original languageEnglish
Pages (from-to)3519 - 3525
Number of pages7
JournalProcess Biochemistry
Volume40
Issue number11
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulases
Trichoderma
Carbon
Enzymes
Steam
Hydrolysis
Zea mays
Salix
Cellulase
acetylxylan esterase
Enzyme activity
Substrates
Glucosidases
hemicellulase
Sugars
Experiments
Proteins

Keywords

  • acetyl xylan esterase
  • alpha-Arabinosidase
  • corn stover
  • Endoglucanase
  • alpha-Galactosidase
  • alpha-Glucosidase
  • Hypocrea jecorina
  • Mannanase
  • alpha-Xylosidase
  • Cellulase
  • Hemicellulase
  • Trichoderma reesei
  • Xylanase

Cite this

Juhasz, T. ; Szengyel, Z. ; Reczey, K. ; Siika-aho, Matti ; Viikari, Liisa. / Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources. In: Process Biochemistry. 2005 ; Vol. 40, No. 11. pp. 3519 - 3525.
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title = "Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources",
abstract = "Cellulase and hemicellulase enzymes were produced by Trichoderma reesei RUT C30 on steam pretreated spruce, willow, corn stover and delignified lignocellulose (Solka Floc), as a reference. The enzymes produced were characterized by protein and various enzyme activity measurements. On steam pretreated corn stover higher cellulolytic enzyme activities were reached than on Solka Floc, while the activities obtained on steam pretreated spruce and willow were considerably lower. The produced and two commercial cellulases (Celluclast 1.5 L and Econase CE) were compared by determining specific activities. There were minor differences among the enzymes corresponding to their specific cellulase activities. In contrast, within hemicellulase and β-glucosidase activities, the differences were found to be more significant. It should be also noted that commercial cellulases had considerably higher specific acetyl xylan esterase activities than the produced enzymes. According to subsequent hydrolysis experiments, performed to characterize the produced enzyme complexes to evaluate their applicability for hydrolysis and enzyme production, it seems that the application of the enzyme that was produced on the same substrate as was used for hydrolysis can be advantageous in the case of some substrates. As a result, these experiments demonstrated that pretreated corn stover is a good substrate both for enzyme production and hydrolysis, since high cellulolytic activities could be reached using it as carbon source. Moreover, high sugar yields could be obtained in the hydrolysis by the enzyme produced on steam pretreated corn stover.",
keywords = "acetyl xylan esterase, alpha-Arabinosidase, corn stover, Endoglucanase, alpha-Galactosidase, alpha-Glucosidase, Hypocrea jecorina, Mannanase, alpha-Xylosidase, Cellulase, Hemicellulase, Trichoderma reesei, Xylanase",
author = "T. Juhasz and Z. Szengyel and K. Reczey and Matti Siika-aho and Liisa Viikari",
year = "2005",
doi = "10.1016/j.procbio.2005.03.057",
language = "English",
volume = "40",
pages = "3519 -- 3525",
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issn = "1359-5113",
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Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources. / Juhasz, T. (Corresponding Author); Szengyel, Z.; Reczey, K.; Siika-aho, Matti; Viikari, Liisa.

In: Process Biochemistry, Vol. 40, No. 11, 2005, p. 3519 - 3525.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources

AU - Juhasz, T.

AU - Szengyel, Z.

AU - Reczey, K.

AU - Siika-aho, Matti

AU - Viikari, Liisa

PY - 2005

Y1 - 2005

N2 - Cellulase and hemicellulase enzymes were produced by Trichoderma reesei RUT C30 on steam pretreated spruce, willow, corn stover and delignified lignocellulose (Solka Floc), as a reference. The enzymes produced were characterized by protein and various enzyme activity measurements. On steam pretreated corn stover higher cellulolytic enzyme activities were reached than on Solka Floc, while the activities obtained on steam pretreated spruce and willow were considerably lower. The produced and two commercial cellulases (Celluclast 1.5 L and Econase CE) were compared by determining specific activities. There were minor differences among the enzymes corresponding to their specific cellulase activities. In contrast, within hemicellulase and β-glucosidase activities, the differences were found to be more significant. It should be also noted that commercial cellulases had considerably higher specific acetyl xylan esterase activities than the produced enzymes. According to subsequent hydrolysis experiments, performed to characterize the produced enzyme complexes to evaluate their applicability for hydrolysis and enzyme production, it seems that the application of the enzyme that was produced on the same substrate as was used for hydrolysis can be advantageous in the case of some substrates. As a result, these experiments demonstrated that pretreated corn stover is a good substrate both for enzyme production and hydrolysis, since high cellulolytic activities could be reached using it as carbon source. Moreover, high sugar yields could be obtained in the hydrolysis by the enzyme produced on steam pretreated corn stover.

AB - Cellulase and hemicellulase enzymes were produced by Trichoderma reesei RUT C30 on steam pretreated spruce, willow, corn stover and delignified lignocellulose (Solka Floc), as a reference. The enzymes produced were characterized by protein and various enzyme activity measurements. On steam pretreated corn stover higher cellulolytic enzyme activities were reached than on Solka Floc, while the activities obtained on steam pretreated spruce and willow were considerably lower. The produced and two commercial cellulases (Celluclast 1.5 L and Econase CE) were compared by determining specific activities. There were minor differences among the enzymes corresponding to their specific cellulase activities. In contrast, within hemicellulase and β-glucosidase activities, the differences were found to be more significant. It should be also noted that commercial cellulases had considerably higher specific acetyl xylan esterase activities than the produced enzymes. According to subsequent hydrolysis experiments, performed to characterize the produced enzyme complexes to evaluate their applicability for hydrolysis and enzyme production, it seems that the application of the enzyme that was produced on the same substrate as was used for hydrolysis can be advantageous in the case of some substrates. As a result, these experiments demonstrated that pretreated corn stover is a good substrate both for enzyme production and hydrolysis, since high cellulolytic activities could be reached using it as carbon source. Moreover, high sugar yields could be obtained in the hydrolysis by the enzyme produced on steam pretreated corn stover.

KW - acetyl xylan esterase

KW - alpha-Arabinosidase

KW - corn stover

KW - Endoglucanase

KW - alpha-Galactosidase

KW - alpha-Glucosidase

KW - Hypocrea jecorina

KW - Mannanase

KW - alpha-Xylosidase

KW - Cellulase

KW - Hemicellulase

KW - Trichoderma reesei

KW - Xylanase

U2 - 10.1016/j.procbio.2005.03.057

DO - 10.1016/j.procbio.2005.03.057

M3 - Article

VL - 40

SP - 3519

EP - 3525

JO - Process Biochemistry

JF - Process Biochemistry

SN - 1359-5113

IS - 11

ER -