Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 142-149 |
| Number of pages | 8 |
| Journal | Journal of Chromatography A |
| Volume | 1293 |
| DOIs | |
| Publication status | Published - 2013 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- cerebrospinal fluid
- comprehensive two-dimensional gas chromatography
- GC * GC-TOFMS
- time-of-flight mass spectrometry
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Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry. / Hartonen, Minna; Mattila, Ismo; Ruskeepää, Anna-Liisa; Orešič, Matej; Hyötyläinen, Tuulia (Corresponding Author).
In: Journal of Chromatography A, Vol. 1293, 2013, p. 142-149.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry
AU - Hartonen, Minna
AU - Mattila, Ismo
AU - Ruskeepää, Anna-Liisa
AU - Orešič, Matej
AU - Hyötyläinen, Tuulia
PY - 2013
Y1 - 2013
N2 - Comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC × GC–TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77 ng/μl in CSF and from 0.1 to 84 ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC × GC–TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.
AB - Comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC × GC–TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77 ng/μl in CSF and from 0.1 to 84 ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC × GC–TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.
KW - cerebrospinal fluid
KW - comprehensive two-dimensional gas chromatography
KW - GC GC-TOFMS
KW - time-of-flight mass spectrometry
U2 - 10.1016/j.chroma.2013.04.005
DO - 10.1016/j.chroma.2013.04.005
M3 - Article
VL - 1293
SP - 142
EP - 149
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -