Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry

Minna Hartonen, Ismo Mattila, Anna-Liisa Ruskeepää, Matej Orešič, Tuulia Hyötyläinen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

Comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC × GC–TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77 ng/μl in CSF and from 0.1 to 84 ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC × GC–TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.
Original languageEnglish
Pages (from-to)142-149
Number of pages8
JournalJournal of Chromatography A
Volume1293
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

Cerebrospinal fluid
Gas chromatography
Gas Chromatography
Mass spectrometry
Cerebrospinal Fluid
Mass Spectrometry
Metabolites
Serum
Amino Acids
Sugar Acids
Metabolome
Organic compounds
Hydroxyl Radical
Functional groups
Gases
Derivatives
Acids

Keywords

  • cerebrospinal fluid
  • comprehensive two-dimensional gas chromatography
  • GC * GC-TOFMS
  • time-of-flight mass spectrometry

Cite this

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title = "Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry",
abstract = "Comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC × GC–TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77 ng/μl in CSF and from 0.1 to 84 ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC × GC–TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.",
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Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry. / Hartonen, Minna; Mattila, Ismo; Ruskeepää, Anna-Liisa; Orešič, Matej; Hyötyläinen, Tuulia (Corresponding Author).

In: Journal of Chromatography A, Vol. 1293, 2013, p. 142-149.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of cerebrospinal fluid by comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry

AU - Hartonen, Minna

AU - Mattila, Ismo

AU - Ruskeepää, Anna-Liisa

AU - Orešič, Matej

AU - Hyötyläinen, Tuulia

PY - 2013

Y1 - 2013

N2 - Comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC × GC–TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77 ng/μl in CSF and from 0.1 to 84 ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC × GC–TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.

AB - Comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (GC × GC–TOFMS) was applied in the quantification and identification of organic compounds in patient-matched human cerebrospinal fluid (CSF) and serum samples. Concentrations of 21 amino and hydroxyl acids varied from 0.04 to 77 ng/μl in CSF and from 0.1 to 84 ng/μl in serum. In total, 91 metabolites out of over 1200 detected were identified based on mass spectra and retention indices. The other metabolites were identified at the functional group level. The main metabolites detected in CSF were sugar and amino acid derivatives. The CSF and serum had clearly distinct metabolic profiles, with larger biological variation in the serum than in CSF. The GC × GC–TOFMS allowed detection and identification of several metabolites that have not been previously detected in CSF.

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KW - GC GC-TOFMS

KW - time-of-flight mass spectrometry

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JO - Journal of Chromatography A

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