TY - JOUR
T1 - Characterization of D-xylose reductase, XyrB, from Aspergillus niger
AU - Terebieniec, Agata
AU - Chroumpi, Tania
AU - Dilokpimol, Adiphol
AU - Aguilar-Pontes, Maria Victoria
AU - Mäkelä, Miia R.
AU - de Vries, Ronald P.
N1 - Funding Information:
TC was supported by a grant of the NWO ALWOP.233 to RPdV. MVAP was supported by a grant of the Dutch Technology Foundation STW , Applied Science division of NWO , and the Technology Program of the Ministry of Economic Affairs 016.130.609 to RPdV. The Academy of Finland grant no. 308284 to MRM is acknowledged. AT was supported by The Polish National Agency For Academic Exchange PROM exchange program and Erasmus Plus exchange program.
Publisher Copyright:
© 2021 The Authors
PY - 2021/6
Y1 - 2021/6
N2 - D-xylose reductase is a member of the aldo-keto reductase family, and is involved in D-xylose and L-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second D-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert D-xylose and L-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).
AB - D-xylose reductase is a member of the aldo-keto reductase family, and is involved in D-xylose and L-arabinose conversion through the Pentose Catabolic Pathway (PCP) in fungi. In this study, we biochemically characterized a newly identified second D-xylose reductase (XyrB) from Aspergillus niger. This NADPH-dependent reductase is able to efficiently convert D-xylose and L-arabinose, and it has the highest affinity for these sugars of all currently known fungal pentose reductases. A combination of biochemical data, transcriptomics and phylogenetic analysis further illustrated the role of XyrB in the PCP. Enzymes: D-xylose reductase (EC 1.1.1.307), L-arabinose reductase (EC 1.1.1.21).
KW - Aspergillus niger
KW - D-xylose reductase
KW - Pentose Catabolic Pathway
UR - http://www.scopus.com/inward/record.url?scp=85102817943&partnerID=8YFLogxK
U2 - 10.1016/j.btre.2021.e00610
DO - 10.1016/j.btre.2021.e00610
M3 - Article
AN - SCOPUS:85102817943
SN - 2215-017X
VL - 30
JO - Biotechnology Reports
JF - Biotechnology Reports
M1 - e00610
ER -