Characterization of fractionated lignins polymerized by fungal laccases

D. van de Pas, A. Hickson, L. Donaldson, G. Lloyd-Jones, Tarja Tamminen, A. Fernyhough, Maija-Liisa Mattinen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

36 Citations (Scopus)

Abstract

Lignins are important biopolymers that can be converted into value-added materials by enzymatic treatments. However, the heterogeneity of the lignin polymer makes it a challenging material to modify. Thus, chemical fractionation was used to obtain lignins with high homogeneity in order to assess their biotechnological utilization. Commercial Alcell, birch organosolv lignins, and steam-exploded pine and eucalypt lignins were sequentially fractionated by ether, ether/acetone 4:1 (v:v), and acetone. All fractions were structurally characterized prior to treatments with Thielavia arenaria, Trametes hirsuta, and Melanocarpus albomyces laccases. The reactivities of the enzymes towards the lignins were determined by oxygen consumption measurements, and the degree of polymerization was confirmed by size exclusion chromatography. Field emission scanning electron microscopy revealed that the surfaces of the lignin nanoparticles were dispersed in the enzyme treatment, suggesting an increase in hydrophilicity of the surfaces detected as loosened morphology. Hence, it was concluded that enzyme-aided valorization is an attractive means for lignin modification, provided that optimum reaction conditions are employed.
Original languageEnglish
Pages (from-to)1105-1121
Number of pages17
JournalBioResources
Volume6
Issue number2
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Laccase
Lignin
lignin
Enzymes
enzyme
Acetone
acetone
ether
Ether
Ethers
Biopolymers
Size exclusion chromatography
Steam
Hydrophilicity
Fractionation
oxygen consumption
polymerization
Field emission
homogeneity
chromatography

Keywords

  • Lignin
  • laccase
  • fractionation
  • modification
  • characterization
  • morphology

Cite this

van de Pas, D., Hickson, A., Donaldson, L., Lloyd-Jones, G., Tamminen, T., Fernyhough, A., & Mattinen, M-L. (2011). Characterization of fractionated lignins polymerized by fungal laccases. BioResources, 6(2), 1105-1121.
van de Pas, D. ; Hickson, A. ; Donaldson, L. ; Lloyd-Jones, G. ; Tamminen, Tarja ; Fernyhough, A. ; Mattinen, Maija-Liisa. / Characterization of fractionated lignins polymerized by fungal laccases. In: BioResources. 2011 ; Vol. 6, No. 2. pp. 1105-1121.
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van de Pas, D, Hickson, A, Donaldson, L, Lloyd-Jones, G, Tamminen, T, Fernyhough, A & Mattinen, M-L 2011, 'Characterization of fractionated lignins polymerized by fungal laccases', BioResources, vol. 6, no. 2, pp. 1105-1121.

Characterization of fractionated lignins polymerized by fungal laccases. / van de Pas, D.; Hickson, A.; Donaldson, L.; Lloyd-Jones, G.; Tamminen, Tarja; Fernyhough, A.; Mattinen, Maija-Liisa (Corresponding Author).

In: BioResources, Vol. 6, No. 2, 2011, p. 1105-1121.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of fractionated lignins polymerized by fungal laccases

AU - van de Pas, D.

AU - Hickson, A.

AU - Donaldson, L.

AU - Lloyd-Jones, G.

AU - Tamminen, Tarja

AU - Fernyhough, A.

AU - Mattinen, Maija-Liisa

PY - 2011

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N2 - Lignins are important biopolymers that can be converted into value-added materials by enzymatic treatments. However, the heterogeneity of the lignin polymer makes it a challenging material to modify. Thus, chemical fractionation was used to obtain lignins with high homogeneity in order to assess their biotechnological utilization. Commercial Alcell, birch organosolv lignins, and steam-exploded pine and eucalypt lignins were sequentially fractionated by ether, ether/acetone 4:1 (v:v), and acetone. All fractions were structurally characterized prior to treatments with Thielavia arenaria, Trametes hirsuta, and Melanocarpus albomyces laccases. The reactivities of the enzymes towards the lignins were determined by oxygen consumption measurements, and the degree of polymerization was confirmed by size exclusion chromatography. Field emission scanning electron microscopy revealed that the surfaces of the lignin nanoparticles were dispersed in the enzyme treatment, suggesting an increase in hydrophilicity of the surfaces detected as loosened morphology. Hence, it was concluded that enzyme-aided valorization is an attractive means for lignin modification, provided that optimum reaction conditions are employed.

AB - Lignins are important biopolymers that can be converted into value-added materials by enzymatic treatments. However, the heterogeneity of the lignin polymer makes it a challenging material to modify. Thus, chemical fractionation was used to obtain lignins with high homogeneity in order to assess their biotechnological utilization. Commercial Alcell, birch organosolv lignins, and steam-exploded pine and eucalypt lignins were sequentially fractionated by ether, ether/acetone 4:1 (v:v), and acetone. All fractions were structurally characterized prior to treatments with Thielavia arenaria, Trametes hirsuta, and Melanocarpus albomyces laccases. The reactivities of the enzymes towards the lignins were determined by oxygen consumption measurements, and the degree of polymerization was confirmed by size exclusion chromatography. Field emission scanning electron microscopy revealed that the surfaces of the lignin nanoparticles were dispersed in the enzyme treatment, suggesting an increase in hydrophilicity of the surfaces detected as loosened morphology. Hence, it was concluded that enzyme-aided valorization is an attractive means for lignin modification, provided that optimum reaction conditions are employed.

KW - Lignin

KW - laccase

KW - fractionation

KW - modification

KW - characterization

KW - morphology

M3 - Article

VL - 6

SP - 1105

EP - 1121

JO - BioResources

JF - BioResources

SN - 1930-2126

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van de Pas D, Hickson A, Donaldson L, Lloyd-Jones G, Tamminen T, Fernyhough A et al. Characterization of fractionated lignins polymerized by fungal laccases. BioResources. 2011;6(2):1105-1121.