Characterization of Leukocyte Formin FMNL1 Expression in Human Tissues

M. Gardberg, V.D. Heuser, Kristiina Iljin, C. Kampf, M. Uhlen, O. Carpén

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Formins are cytoskeleton regulating proteins characterized by a common FH2 structural domain. As key players in the assembly of actin filaments, formins direct dynamic cytoskeletal processes that influence cell shape, movement and adhesion. The large number of formin genes, fifteen in the human, suggests distinct tasks and expression patterns for individual family members, in addition to overlapping functions. Several formins have been associated with invasive cell properties in experimental models, linking them to cancer biology. One example is FMNL1, which is considered to be a leukocyte formin and is known to be overexpressed in lymphomas. Studies on FMNL1 and many other formins have been hampered by a lack of research tools, especially antibodies suitable for staining paraffin-embedded formalin-fixed tissues. Here we characterize, using bioinformatics tools and a validated antibody, the expression pattern of FMNL1 in human tissues and study its subcellular distribution. Our results indicate that FMNL1 expression is not restricted to hematopoietic tissues and that neoexpression of FMNL1 can be seen in epithelial cancer.
Original languageEnglish
Pages (from-to)460-470
JournalJournal of Histochemistry and Cytochemistry
Volume62
Issue number6
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Leukocytes
Cell Shape
Antibodies
Computational Biology
Cytoskeleton
Actin Cytoskeleton
Cell Adhesion
Paraffin
Formaldehyde
Cell Movement
Lymphoma
Neoplasms
Theoretical Models
Staining and Labeling
Research
Genes
Proteins

Keywords

  • actin cytoskeleton
  • immunohistochemistry
  • formins
  • FMNL1
  • FRL1

Cite this

Gardberg, M. ; Heuser, V.D. ; Iljin, Kristiina ; Kampf, C. ; Uhlen, M. ; Carpén, O. / Characterization of Leukocyte Formin FMNL1 Expression in Human Tissues. In: Journal of Histochemistry and Cytochemistry. 2014 ; Vol. 62, No. 6. pp. 460-470.
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abstract = "Formins are cytoskeleton regulating proteins characterized by a common FH2 structural domain. As key players in the assembly of actin filaments, formins direct dynamic cytoskeletal processes that influence cell shape, movement and adhesion. The large number of formin genes, fifteen in the human, suggests distinct tasks and expression patterns for individual family members, in addition to overlapping functions. Several formins have been associated with invasive cell properties in experimental models, linking them to cancer biology. One example is FMNL1, which is considered to be a leukocyte formin and is known to be overexpressed in lymphomas. Studies on FMNL1 and many other formins have been hampered by a lack of research tools, especially antibodies suitable for staining paraffin-embedded formalin-fixed tissues. Here we characterize, using bioinformatics tools and a validated antibody, the expression pattern of FMNL1 in human tissues and study its subcellular distribution. Our results indicate that FMNL1 expression is not restricted to hematopoietic tissues and that neoexpression of FMNL1 can be seen in epithelial cancer.",
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Characterization of Leukocyte Formin FMNL1 Expression in Human Tissues. / Gardberg, M.; Heuser, V.D.; Iljin, Kristiina; Kampf, C.; Uhlen, M.; Carpén, O.

In: Journal of Histochemistry and Cytochemistry, Vol. 62, No. 6, 2014, p. 460-470.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of Leukocyte Formin FMNL1 Expression in Human Tissues

AU - Gardberg, M.

AU - Heuser, V.D.

AU - Iljin, Kristiina

AU - Kampf, C.

AU - Uhlen, M.

AU - Carpén, O.

PY - 2014

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AB - Formins are cytoskeleton regulating proteins characterized by a common FH2 structural domain. As key players in the assembly of actin filaments, formins direct dynamic cytoskeletal processes that influence cell shape, movement and adhesion. The large number of formin genes, fifteen in the human, suggests distinct tasks and expression patterns for individual family members, in addition to overlapping functions. Several formins have been associated with invasive cell properties in experimental models, linking them to cancer biology. One example is FMNL1, which is considered to be a leukocyte formin and is known to be overexpressed in lymphomas. Studies on FMNL1 and many other formins have been hampered by a lack of research tools, especially antibodies suitable for staining paraffin-embedded formalin-fixed tissues. Here we characterize, using bioinformatics tools and a validated antibody, the expression pattern of FMNL1 in human tissues and study its subcellular distribution. Our results indicate that FMNL1 expression is not restricted to hematopoietic tissues and that neoexpression of FMNL1 can be seen in epithelial cancer.

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