Characterization of lipids and lignans in brewer's spent grain and its enzymatically extracted fraction

Piritta Niemi (Corresponding Author), Tarja Tamminen, Annika Smeds, Kaarina Viljanen, Taina Ohra-aho, Ulla Holopainen-Mantila, Craig B. Faulds, Kaisa Poutanen, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

44 Citations (Scopus)


Brewer's spent grain (BSG), the major side stream of brewing, consists of the husks and the residual parts of malts after the mashing process. BSG was enzymatically fractionated by a two-step treatment with carbohydrate- and protein-degrading enzymes, which solubilized 66% of BSG. BSG contained 11% lipids, which were mostly triglycerides, but also a notable amount of free fatty acids was present. Lipids were mostly solubilized due to the alkaline pH applied in the protease treatment. The main fatty acids were linoleic, palmitic, and oleic acids. Several lignans were identified in BSG, syringaresinol and secoisolariciresinol being the most abundant, many associated with the cell wall matrix and released by the alkaline-protease treatment.
Original languageEnglish
Pages (from-to)9910-9917
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Issue number39
Publication statusPublished - Oct 2012
MoE publication typeA1 Journal article-refereed


  • Cereals
  • Cereals: chemistry
  • Enzymes
  • Enzymes: chemistry
  • Fatty Acids
  • Fatty Acids: chemistry
  • Fermentation
  • Industrial Waste
  • Industrial Waste: analysis
  • Lignans
  • Lignans: chemistry
  • Lipids
  • Lipids: chemistry

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