Characterization of lipopolysaccharides of polymyxin-resistant and polymyxin-sensitive Klebsiella pneumoniae O3

Ilkka Helander, Yutaka Kato, Ilkka Kilpeläinen, Risto Kostiainen, Buko Lindner, Kim Nummila, Tsuyoshi Sugiyama, Takashi Yokochi

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Abstract

Lipopolysaccharides isolated from the polymyxin‐resistant Klebsiella pneumoniae 03 mutant OM‐5 and its polymyxin‐sensitive parent LEN‐1 were analyzed for chemical composition, and their lipid A portions were structurally characterized. The lipopolysaccharide of OM‐5 contained approximately five times more 4‐amino‐4‐deoxy‐l‐arabinopyranose than that of LEN‐1. Other saccharide and phosphate components exhibited no significant differences. Structural characterization, including analyses by phosphorus magnetic resonance spectroscopy and by fast atom bombardment mass spectrometry, revealed a novel type of lipid A. In the OM‐5 lipopolysaccharide, both phosphates of lipid A were almost totally present as phosphodiesters with 4‐amino‐4‐deoxy‐l‐arabinopyranose. In the sensitive‐type LEN‐1 lipid A, the extent of this substitution was much lower, especially in the glycosidically linked phosphate. Phosphate in these K. pneumoniae lipopolysaccharides was almost exclusively found in lipid A. These results show that cationic substituents of phosphates of lipid A play a decisive role in determining polymyxin reactivity. OM‐5 was also found to contain a large proportion of heptaacyl lipid A, which represented only a small fraction of lipid A in LEN‐1.
Original languageEnglish
Pages (from-to)272-278
Number of pages7
JournalEuropean Journal of Biochemistry
Volume237
Issue number1
DOIs
Publication statusPublished - 1996
MoE publication typeA1 Journal article-refereed

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Polymyxins
Lipid A
Klebsiella pneumoniae
Lipopolysaccharides
Phosphates
Magnetic resonance spectroscopy
Fast Atom Bombardment Mass Spectrometry
Phosphorus
Mass spectrometry
Substitution reactions
Magnetic Resonance Spectroscopy
Atoms

Cite this

Helander, I., Kato, Y., Kilpeläinen, I., Kostiainen, R., Lindner, B., Nummila, K., ... Yokochi, T. (1996). Characterization of lipopolysaccharides of polymyxin-resistant and polymyxin-sensitive Klebsiella pneumoniae O3. European Journal of Biochemistry, 237(1), 272-278. https://doi.org/10.1111/j.1432-1033.1996.0272n.x
Helander, Ilkka ; Kato, Yutaka ; Kilpeläinen, Ilkka ; Kostiainen, Risto ; Lindner, Buko ; Nummila, Kim ; Sugiyama, Tsuyoshi ; Yokochi, Takashi. / Characterization of lipopolysaccharides of polymyxin-resistant and polymyxin-sensitive Klebsiella pneumoniae O3. In: European Journal of Biochemistry. 1996 ; Vol. 237, No. 1. pp. 272-278.
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title = "Characterization of lipopolysaccharides of polymyxin-resistant and polymyxin-sensitive Klebsiella pneumoniae O3",
abstract = "Lipopolysaccharides isolated from the polymyxin‐resistant Klebsiella pneumoniae 03 mutant OM‐5 and its polymyxin‐sensitive parent LEN‐1 were analyzed for chemical composition, and their lipid A portions were structurally characterized. The lipopolysaccharide of OM‐5 contained approximately five times more 4‐amino‐4‐deoxy‐l‐arabinopyranose than that of LEN‐1. Other saccharide and phosphate components exhibited no significant differences. Structural characterization, including analyses by phosphorus magnetic resonance spectroscopy and by fast atom bombardment mass spectrometry, revealed a novel type of lipid A. In the OM‐5 lipopolysaccharide, both phosphates of lipid A were almost totally present as phosphodiesters with 4‐amino‐4‐deoxy‐l‐arabinopyranose. In the sensitive‐type LEN‐1 lipid A, the extent of this substitution was much lower, especially in the glycosidically linked phosphate. Phosphate in these K. pneumoniae lipopolysaccharides was almost exclusively found in lipid A. These results show that cationic substituents of phosphates of lipid A play a decisive role in determining polymyxin reactivity. OM‐5 was also found to contain a large proportion of heptaacyl lipid A, which represented only a small fraction of lipid A in LEN‐1.",
author = "Ilkka Helander and Yutaka Kato and Ilkka Kilpel{\"a}inen and Risto Kostiainen and Buko Lindner and Kim Nummila and Tsuyoshi Sugiyama and Takashi Yokochi",
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Helander, I, Kato, Y, Kilpeläinen, I, Kostiainen, R, Lindner, B, Nummila, K, Sugiyama, T & Yokochi, T 1996, 'Characterization of lipopolysaccharides of polymyxin-resistant and polymyxin-sensitive Klebsiella pneumoniae O3', European Journal of Biochemistry, vol. 237, no. 1, pp. 272-278. https://doi.org/10.1111/j.1432-1033.1996.0272n.x

Characterization of lipopolysaccharides of polymyxin-resistant and polymyxin-sensitive Klebsiella pneumoniae O3. / Helander, Ilkka; Kato, Yutaka; Kilpeläinen, Ilkka; Kostiainen, Risto; Lindner, Buko; Nummila, Kim; Sugiyama, Tsuyoshi; Yokochi, Takashi.

In: European Journal of Biochemistry, Vol. 237, No. 1, 1996, p. 272-278.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of lipopolysaccharides of polymyxin-resistant and polymyxin-sensitive Klebsiella pneumoniae O3

AU - Helander, Ilkka

AU - Kato, Yutaka

AU - Kilpeläinen, Ilkka

AU - Kostiainen, Risto

AU - Lindner, Buko

AU - Nummila, Kim

AU - Sugiyama, Tsuyoshi

AU - Yokochi, Takashi

PY - 1996

Y1 - 1996

N2 - Lipopolysaccharides isolated from the polymyxin‐resistant Klebsiella pneumoniae 03 mutant OM‐5 and its polymyxin‐sensitive parent LEN‐1 were analyzed for chemical composition, and their lipid A portions were structurally characterized. The lipopolysaccharide of OM‐5 contained approximately five times more 4‐amino‐4‐deoxy‐l‐arabinopyranose than that of LEN‐1. Other saccharide and phosphate components exhibited no significant differences. Structural characterization, including analyses by phosphorus magnetic resonance spectroscopy and by fast atom bombardment mass spectrometry, revealed a novel type of lipid A. In the OM‐5 lipopolysaccharide, both phosphates of lipid A were almost totally present as phosphodiesters with 4‐amino‐4‐deoxy‐l‐arabinopyranose. In the sensitive‐type LEN‐1 lipid A, the extent of this substitution was much lower, especially in the glycosidically linked phosphate. Phosphate in these K. pneumoniae lipopolysaccharides was almost exclusively found in lipid A. These results show that cationic substituents of phosphates of lipid A play a decisive role in determining polymyxin reactivity. OM‐5 was also found to contain a large proportion of heptaacyl lipid A, which represented only a small fraction of lipid A in LEN‐1.

AB - Lipopolysaccharides isolated from the polymyxin‐resistant Klebsiella pneumoniae 03 mutant OM‐5 and its polymyxin‐sensitive parent LEN‐1 were analyzed for chemical composition, and their lipid A portions were structurally characterized. The lipopolysaccharide of OM‐5 contained approximately five times more 4‐amino‐4‐deoxy‐l‐arabinopyranose than that of LEN‐1. Other saccharide and phosphate components exhibited no significant differences. Structural characterization, including analyses by phosphorus magnetic resonance spectroscopy and by fast atom bombardment mass spectrometry, revealed a novel type of lipid A. In the OM‐5 lipopolysaccharide, both phosphates of lipid A were almost totally present as phosphodiesters with 4‐amino‐4‐deoxy‐l‐arabinopyranose. In the sensitive‐type LEN‐1 lipid A, the extent of this substitution was much lower, especially in the glycosidically linked phosphate. Phosphate in these K. pneumoniae lipopolysaccharides was almost exclusively found in lipid A. These results show that cationic substituents of phosphates of lipid A play a decisive role in determining polymyxin reactivity. OM‐5 was also found to contain a large proportion of heptaacyl lipid A, which represented only a small fraction of lipid A in LEN‐1.

U2 - 10.1111/j.1432-1033.1996.0272n.x

DO - 10.1111/j.1432-1033.1996.0272n.x

M3 - Article

VL - 237

SP - 272

EP - 278

JO - FEBS Journal

JF - FEBS Journal

SN - 1742-464X

IS - 1

ER -