Characterization of Listeria monocytogenes isolates from the meat, poultry and seafood industries by automated ribotyping

Maija-Liisa Suihko (Corresponding Author), Satu Salo, Oluva Niclasen, Birna Gudbjörnsdottir, Gudjon Torkelsson, Sylvia Bredholt, Anna-Maija Sjöberg, Patrick Gustavsson

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

A total of 564 Listeria monocytogenes isolates were characterized by automated ribotyping. The samples were taken from equipment, personnel and the environment after cleaning procedures and during food processing, as well as from raw materials and products from six meat, two poultry and five seafood processing plants located in the Faroe Islands, Finland, Iceland, Norway and Sweden. Altogether, 25 different ribotypes (RTs) were generated. Two RTs occurred in the samples from all three food sectors—meat, poultry and seafood. Four RTs occurred in meat and poultry plant samples and other four RTs occurred in meat and seafood plant samples. Five RTs occurred only in meat plant samples, five only in poultry plant samples and five only in seafood plant samples. Eight of the thirteen plants had their own in-house L. monocytogenes ribotype. There was geographical differences between the RTs, but no correlation between RTs and food sectors was detected. The discrimination power of automated ribotyping was satisfactory to trace the contamination sources in the food processing plants clearly indicating the sites at which improved cleaning procedures were necessary. In addition, it was possible to screen a large number of isolates with two instruments located at different institutes and to make a reliable combination of the results.
Original languageEnglish
Pages (from-to)137-146
JournalInternational Journal of Food Microbiology
Volume72
Issue number1-2
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Ribotyping
ribotypes
Seafood
fish industry
livestock and meat industry
poultry industry
Listeria monocytogenes
Poultry
DNA fingerprinting
Meat
Industry
poultry
seafoods
meat
food processing plants
sampling
Food Handling
cleaning
Faroe Islands
Iceland

Keywords

  • Listeria monocytogenes
  • ribotyping
  • meat
  • poultry
  • seafood

Cite this

Suihko, Maija-Liisa ; Salo, Satu ; Niclasen, Oluva ; Gudbjörnsdottir, Birna ; Torkelsson, Gudjon ; Bredholt, Sylvia ; Sjöberg, Anna-Maija ; Gustavsson, Patrick. / Characterization of Listeria monocytogenes isolates from the meat, poultry and seafood industries by automated ribotyping. In: International Journal of Food Microbiology. 2002 ; Vol. 72, No. 1-2. pp. 137-146.
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abstract = "A total of 564 Listeria monocytogenes isolates were characterized by automated ribotyping. The samples were taken from equipment, personnel and the environment after cleaning procedures and during food processing, as well as from raw materials and products from six meat, two poultry and five seafood processing plants located in the Faroe Islands, Finland, Iceland, Norway and Sweden. Altogether, 25 different ribotypes (RTs) were generated. Two RTs occurred in the samples from all three food sectors—meat, poultry and seafood. Four RTs occurred in meat and poultry plant samples and other four RTs occurred in meat and seafood plant samples. Five RTs occurred only in meat plant samples, five only in poultry plant samples and five only in seafood plant samples. Eight of the thirteen plants had their own in-house L. monocytogenes ribotype. There was geographical differences between the RTs, but no correlation between RTs and food sectors was detected. The discrimination power of automated ribotyping was satisfactory to trace the contamination sources in the food processing plants clearly indicating the sites at which improved cleaning procedures were necessary. In addition, it was possible to screen a large number of isolates with two instruments located at different institutes and to make a reliable combination of the results.",
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Suihko, M-L, Salo, S, Niclasen, O, Gudbjörnsdottir, B, Torkelsson, G, Bredholt, S, Sjöberg, A-M & Gustavsson, P 2002, 'Characterization of Listeria monocytogenes isolates from the meat, poultry and seafood industries by automated ribotyping', International Journal of Food Microbiology, vol. 72, no. 1-2, pp. 137-146. https://doi.org/10.1016/S0168-1605(01)00631-6

Characterization of Listeria monocytogenes isolates from the meat, poultry and seafood industries by automated ribotyping. / Suihko, Maija-Liisa (Corresponding Author); Salo, Satu; Niclasen, Oluva; Gudbjörnsdottir, Birna; Torkelsson, Gudjon; Bredholt, Sylvia; Sjöberg, Anna-Maija; Gustavsson, Patrick.

In: International Journal of Food Microbiology, Vol. 72, No. 1-2, 2002, p. 137-146.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of Listeria monocytogenes isolates from the meat, poultry and seafood industries by automated ribotyping

AU - Suihko, Maija-Liisa

AU - Salo, Satu

AU - Niclasen, Oluva

AU - Gudbjörnsdottir, Birna

AU - Torkelsson, Gudjon

AU - Bredholt, Sylvia

AU - Sjöberg, Anna-Maija

AU - Gustavsson, Patrick

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N2 - A total of 564 Listeria monocytogenes isolates were characterized by automated ribotyping. The samples were taken from equipment, personnel and the environment after cleaning procedures and during food processing, as well as from raw materials and products from six meat, two poultry and five seafood processing plants located in the Faroe Islands, Finland, Iceland, Norway and Sweden. Altogether, 25 different ribotypes (RTs) were generated. Two RTs occurred in the samples from all three food sectors—meat, poultry and seafood. Four RTs occurred in meat and poultry plant samples and other four RTs occurred in meat and seafood plant samples. Five RTs occurred only in meat plant samples, five only in poultry plant samples and five only in seafood plant samples. Eight of the thirteen plants had their own in-house L. monocytogenes ribotype. There was geographical differences between the RTs, but no correlation between RTs and food sectors was detected. The discrimination power of automated ribotyping was satisfactory to trace the contamination sources in the food processing plants clearly indicating the sites at which improved cleaning procedures were necessary. In addition, it was possible to screen a large number of isolates with two instruments located at different institutes and to make a reliable combination of the results.

AB - A total of 564 Listeria monocytogenes isolates were characterized by automated ribotyping. The samples were taken from equipment, personnel and the environment after cleaning procedures and during food processing, as well as from raw materials and products from six meat, two poultry and five seafood processing plants located in the Faroe Islands, Finland, Iceland, Norway and Sweden. Altogether, 25 different ribotypes (RTs) were generated. Two RTs occurred in the samples from all three food sectors—meat, poultry and seafood. Four RTs occurred in meat and poultry plant samples and other four RTs occurred in meat and seafood plant samples. Five RTs occurred only in meat plant samples, five only in poultry plant samples and five only in seafood plant samples. Eight of the thirteen plants had their own in-house L. monocytogenes ribotype. There was geographical differences between the RTs, but no correlation between RTs and food sectors was detected. The discrimination power of automated ribotyping was satisfactory to trace the contamination sources in the food processing plants clearly indicating the sites at which improved cleaning procedures were necessary. In addition, it was possible to screen a large number of isolates with two instruments located at different institutes and to make a reliable combination of the results.

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KW - poultry

KW - seafood

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DO - 10.1016/S0168-1605(01)00631-6

M3 - Article

VL - 72

SP - 137

EP - 146

JO - International Journal of Food Microbiology

JF - International Journal of Food Microbiology

SN - 0168-1605

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