Characterization of microstructure and cell wall components of Arabidopsis thaliana overexpressing cyclin-dependent kinase inhibitor 2

Marjatta Salmenkallio-Marttila, Anna-Maria Aura, L. De Veylder, Dirk Inze, Kirsi-Marja Oksman-Caldentey

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Overexpression of a cyclin-dependent kinase inhibitor (KRP2) caused changes in the general morphology in the leaves of Arabidopsis thaliana. The wild type plant had obovate leaves with entire margins whereas the transgenic line had leaves with denticulate margins. The epidermal cells and stomata of the adult transgenic leaves were significantly larger than those of the wild-type plants na ll cells between the two samples were observed. The smaller amount of cell wal l material in the transgenic leaves caused by the larger cell size was also apparent in the lower dry weight of the transgenic leaves. The chemical analysis revealed the main differences to be in pectin and neutral sugar contents, and especially in the amounts of glucose, all being higher in the leaves of the KRP2 transgenic plants. p-Coumaric acid content varied more in the transgenic leaf material than in the control one reflecting possibly fewer cross-links in the cell walls of transgenic plants.
Original languageEnglish
Pages (from-to)93-99
JournalPhytochemistry Reviews
Volume1
Issue number1
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Cyclin-Dependent Kinase 2
cyclin-dependent kinase
cell wall components
Cellular Structures
Arabidopsis
Cell Wall
microstructure
Arabidopsis thaliana
Genetically Modified Plants
genetically modified organisms
leaves
Cyclin-Dependent Kinases
Cell Size
Weights and Measures
Glucose
transgenic plants
cells
p-coumaric acid
chemical analysis
stomata

Keywords

  • Arabidopsis thaliana
  • cell cycle modulation
  • cell wall
  • cyclin-dependent kinase inhibitor
  • genetic engineering
  • leaf morphology

Cite this

@article{aba9fb2a15fc4aaeb17a0596e0df7e3a,
title = "Characterization of microstructure and cell wall components of Arabidopsis thaliana overexpressing cyclin-dependent kinase inhibitor 2",
abstract = "Overexpression of a cyclin-dependent kinase inhibitor (KRP2) caused changes in the general morphology in the leaves of Arabidopsis thaliana. The wild type plant had obovate leaves with entire margins whereas the transgenic line had leaves with denticulate margins. The epidermal cells and stomata of the adult transgenic leaves were significantly larger than those of the wild-type plants na ll cells between the two samples were observed. The smaller amount of cell wal l material in the transgenic leaves caused by the larger cell size was also apparent in the lower dry weight of the transgenic leaves. The chemical analysis revealed the main differences to be in pectin and neutral sugar contents, and especially in the amounts of glucose, all being higher in the leaves of the KRP2 transgenic plants. p-Coumaric acid content varied more in the transgenic leaf material than in the control one reflecting possibly fewer cross-links in the cell walls of transgenic plants.",
keywords = "Arabidopsis thaliana, cell cycle modulation, cell wall, cyclin-dependent kinase inhibitor, genetic engineering, leaf morphology",
author = "Marjatta Salmenkallio-Marttila and Anna-Maria Aura and {De Veylder}, L. and Dirk Inze and Kirsi-Marja Oksman-Caldentey",
year = "2002",
doi = "10.1023/A:1015840521376",
language = "English",
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journal = "Phytochemistry Reviews",
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Characterization of microstructure and cell wall components of Arabidopsis thaliana overexpressing cyclin-dependent kinase inhibitor 2. / Salmenkallio-Marttila, Marjatta; Aura, Anna-Maria; De Veylder, L.; Inze, Dirk; Oksman-Caldentey, Kirsi-Marja.

In: Phytochemistry Reviews, Vol. 1, No. 1, 2002, p. 93-99.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of microstructure and cell wall components of Arabidopsis thaliana overexpressing cyclin-dependent kinase inhibitor 2

AU - Salmenkallio-Marttila, Marjatta

AU - Aura, Anna-Maria

AU - De Veylder, L.

AU - Inze, Dirk

AU - Oksman-Caldentey, Kirsi-Marja

PY - 2002

Y1 - 2002

N2 - Overexpression of a cyclin-dependent kinase inhibitor (KRP2) caused changes in the general morphology in the leaves of Arabidopsis thaliana. The wild type plant had obovate leaves with entire margins whereas the transgenic line had leaves with denticulate margins. The epidermal cells and stomata of the adult transgenic leaves were significantly larger than those of the wild-type plants na ll cells between the two samples were observed. The smaller amount of cell wal l material in the transgenic leaves caused by the larger cell size was also apparent in the lower dry weight of the transgenic leaves. The chemical analysis revealed the main differences to be in pectin and neutral sugar contents, and especially in the amounts of glucose, all being higher in the leaves of the KRP2 transgenic plants. p-Coumaric acid content varied more in the transgenic leaf material than in the control one reflecting possibly fewer cross-links in the cell walls of transgenic plants.

AB - Overexpression of a cyclin-dependent kinase inhibitor (KRP2) caused changes in the general morphology in the leaves of Arabidopsis thaliana. The wild type plant had obovate leaves with entire margins whereas the transgenic line had leaves with denticulate margins. The epidermal cells and stomata of the adult transgenic leaves were significantly larger than those of the wild-type plants na ll cells between the two samples were observed. The smaller amount of cell wal l material in the transgenic leaves caused by the larger cell size was also apparent in the lower dry weight of the transgenic leaves. The chemical analysis revealed the main differences to be in pectin and neutral sugar contents, and especially in the amounts of glucose, all being higher in the leaves of the KRP2 transgenic plants. p-Coumaric acid content varied more in the transgenic leaf material than in the control one reflecting possibly fewer cross-links in the cell walls of transgenic plants.

KW - Arabidopsis thaliana

KW - cell cycle modulation

KW - cell wall

KW - cyclin-dependent kinase inhibitor

KW - genetic engineering

KW - leaf morphology

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SN - 1568-7767

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