Characterization of novel thermostable fungal cellobiohydrolases

T. Puranen, Sanni Voutilainen, Matti Siika-aho, J. Kallio, Satu Hooman, Liisa Viikari, Anu Koivula, J. Vehmaanperä

    Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

    Abstract

    Cellulose degradation requires sequential or simultaneous synergic action of three types of hydrolytic enzymes: cellobiohydrolases, endoglucanases and beta-glucosidases. Various filamentous fungi produce these enzymes in order to hydrolyze insoluble cellulose into glucose. Cellulases are currently extensively studied in enzyme industry for cellulosic biomass conversion to ethanol. Here, molecular cloning of three different cellobiohydrolase genes from thermophilic ascomycetes is presented together with their heterologous expression in Trichoderma reesei. The recombinant cellobiohydrolases that belong to the glycosyl hydrolase (GH) family 7 were purified and characterized in terms of pH optimum, thermal stability and kinetic parameters. Thermostable cellulases such as described here have been proposed to improve the overall process economy of the biomass conversion with favourable impact on enzyme need, hydrolysis performance and flexibility of the process.
    Original languageEnglish
    Title of host publication3rd European Federation of Biotechnology Conference
    Subtitle of host publicationPhysiology of Yeasts and Filamentous Fungi PYFF3. Kuokka-Ihalainen
    Place of PublicationEspoo
    PublisherVTT Technical Research Centre of Finland
    Pages145
    ISBN (Electronic)978-951-38-6314-2
    ISBN (Print)978-951-38-6313-5
    Publication statusPublished - 2007
    Event3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi - Helsinki, Finland
    Duration: 13 Jun 200716 Jun 2007

    Publication series

    SeriesVTT Symposium
    Number245
    ISSN0357-9387

    Conference

    Conference3rd European Federation of Biotechnology Conference
    Abbreviated titlePYFF3
    Country/TerritoryFinland
    CityHelsinki
    Period13/06/0716/06/07

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