@inbook{ccc3a0c7333e45b884f4c8c7b1227147,
title = "Characterization of novel thermostable fungal cellobiohydrolases",
abstract = "Cellulose degradation requires sequential or simultaneous synergic action of three types of hydrolytic enzymes: cellobiohydrolases, endoglucanases and beta-glucosidases. Various filamentous fungi produce these enzymes in order to hydrolyze insoluble cellulose into glucose. Cellulases are currently extensively studied in enzyme industry for cellulosic biomass conversion to ethanol. Here, molecular cloning of three different cellobiohydrolase genes from thermophilic ascomycetes is presented together with their heterologous expression in Trichoderma reesei. The recombinant cellobiohydrolases that belong to the glycosyl hydrolase (GH) family 7 were purified and characterized in terms of pH optimum, thermal stability and kinetic parameters. Thermostable cellulases such as described here have been proposed to improve the overall process economy of the biomass conversion with favourable impact on enzyme need, hydrolysis performance and flexibility of the process.",
author = "T. Puranen and Sanni Voutilainen and Matti Siika-aho and J. Kallio and Satu Hooman and Liisa Viikari and Anu Koivula and J. Vehmaanper{\"a}",
year = "2007",
language = "English",
isbn = "978-951-38-6313-5",
series = "VTT Symposium",
publisher = "VTT Technical Research Centre of Finland",
number = "245",
pages = "145",
booktitle = "3rd European Federation of Biotechnology Conference",
address = "Finland",
note = "3rd European Federation of Biotechnology Conference : Physiology of Yeasts and Filamentous Fungi, PYFF3 ; Conference date: 13-06-2007 Through 16-06-2007",
}