Characterization of O-acetyl-(4-O-methylglucurono)xylan isolated from birch and beech

Anita Teleman (Corresponding Author), Maija Tenkanen, Anna Jacobs, Olof Dahlman

Research output: Contribution to journalArticleScientificpeer-review

173 Citations (Scopus)

Abstract

The structures of water-soluble birch and beech xylans, extracted from holocellulose using dimethyl sulfoxide, were determined employing 1H and 13C NMR spectroscopy together with chemical analysis.
These polysaccharides were found to be O-acetyl-(4-O-methylglucurono)xylans containing one 4-O-methylglucuronic acid substituent for approximately every 15 d-xylose residues. The average degree of acetylation of the xylose residues in these polymers was 0.4.
The presence of the structural element →4)[4-O-Me-α-d-GlcpA-(1→2)][3-O-Ac]-β-d-Xylp-(1→ was demonstrated. Additional acetyl groups were present as substituents at C-2 and/or C-3 of the xylopyranosyl residues. Utilizing size-exclusion chromatography in combination with mass spectroscopy, the weight-average molar masses (and polydispersities) were shown to be 8000 (1.09) and 11,100 (1.08) for birch and beech xylan, respectively.
Original languageEnglish
Pages (from-to)373-377
JournalCarbohydrate Research
Volume337
Issue number4
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Fagus
Xylans
Betula
Xylose
Acetylation
Size exclusion chromatography
Molar mass
Polydispersity
Dimethyl Sulfoxide
Nuclear magnetic resonance spectroscopy
Gel Chromatography
Polysaccharides
Mass Spectrometry
Polymers
Magnetic Resonance Spectroscopy
Spectroscopy
Weights and Measures
Water
Chemical analysis
O-acetyl-(4-O-methylglucurono)xylan

Keywords

  • hardwood
  • hemicellulose
  • extraction
  • xylan
  • structure
  • O-acetylation
  • 1H and 13C NMR spectroscopy
  • MALDI-MS

Cite this

Teleman, Anita ; Tenkanen, Maija ; Jacobs, Anna ; Dahlman, Olof. / Characterization of O-acetyl-(4-O-methylglucurono)xylan isolated from birch and beech. In: Carbohydrate Research. 2002 ; Vol. 337, No. 4. pp. 373-377.
@article{dfbbbb42c4e34c12adcf414381a03ca0,
title = "Characterization of O-acetyl-(4-O-methylglucurono)xylan isolated from birch and beech",
abstract = "The structures of water-soluble birch and beech xylans, extracted from holocellulose using dimethyl sulfoxide, were determined employing 1H and 13C NMR spectroscopy together with chemical analysis. These polysaccharides were found to be O-acetyl-(4-O-methylglucurono)xylans containing one 4-O-methylglucuronic acid substituent for approximately every 15 d-xylose residues. The average degree of acetylation of the xylose residues in these polymers was 0.4. The presence of the structural element →4)[4-O-Me-α-d-GlcpA-(1→2)][3-O-Ac]-β-d-Xylp-(1→ was demonstrated. Additional acetyl groups were present as substituents at C-2 and/or C-3 of the xylopyranosyl residues. Utilizing size-exclusion chromatography in combination with mass spectroscopy, the weight-average molar masses (and polydispersities) were shown to be 8000 (1.09) and 11,100 (1.08) for birch and beech xylan, respectively.",
keywords = "hardwood, hemicellulose, extraction, xylan, structure, O-acetylation, 1H and 13C NMR spectroscopy, MALDI-MS",
author = "Anita Teleman and Maija Tenkanen and Anna Jacobs and Olof Dahlman",
year = "2002",
doi = "10.1016/S0008-6215(01)00327-5",
language = "English",
volume = "337",
pages = "373--377",
journal = "Carbohydrate Research",
issn = "0008-6215",
publisher = "Elsevier",
number = "4",

}

Characterization of O-acetyl-(4-O-methylglucurono)xylan isolated from birch and beech. / Teleman, Anita (Corresponding Author); Tenkanen, Maija; Jacobs, Anna; Dahlman, Olof.

In: Carbohydrate Research, Vol. 337, No. 4, 2002, p. 373-377.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of O-acetyl-(4-O-methylglucurono)xylan isolated from birch and beech

AU - Teleman, Anita

AU - Tenkanen, Maija

AU - Jacobs, Anna

AU - Dahlman, Olof

PY - 2002

Y1 - 2002

N2 - The structures of water-soluble birch and beech xylans, extracted from holocellulose using dimethyl sulfoxide, were determined employing 1H and 13C NMR spectroscopy together with chemical analysis. These polysaccharides were found to be O-acetyl-(4-O-methylglucurono)xylans containing one 4-O-methylglucuronic acid substituent for approximately every 15 d-xylose residues. The average degree of acetylation of the xylose residues in these polymers was 0.4. The presence of the structural element →4)[4-O-Me-α-d-GlcpA-(1→2)][3-O-Ac]-β-d-Xylp-(1→ was demonstrated. Additional acetyl groups were present as substituents at C-2 and/or C-3 of the xylopyranosyl residues. Utilizing size-exclusion chromatography in combination with mass spectroscopy, the weight-average molar masses (and polydispersities) were shown to be 8000 (1.09) and 11,100 (1.08) for birch and beech xylan, respectively.

AB - The structures of water-soluble birch and beech xylans, extracted from holocellulose using dimethyl sulfoxide, were determined employing 1H and 13C NMR spectroscopy together with chemical analysis. These polysaccharides were found to be O-acetyl-(4-O-methylglucurono)xylans containing one 4-O-methylglucuronic acid substituent for approximately every 15 d-xylose residues. The average degree of acetylation of the xylose residues in these polymers was 0.4. The presence of the structural element →4)[4-O-Me-α-d-GlcpA-(1→2)][3-O-Ac]-β-d-Xylp-(1→ was demonstrated. Additional acetyl groups were present as substituents at C-2 and/or C-3 of the xylopyranosyl residues. Utilizing size-exclusion chromatography in combination with mass spectroscopy, the weight-average molar masses (and polydispersities) were shown to be 8000 (1.09) and 11,100 (1.08) for birch and beech xylan, respectively.

KW - hardwood

KW - hemicellulose

KW - extraction

KW - xylan

KW - structure

KW - O-acetylation

KW - 1H and 13C NMR spectroscopy

KW - MALDI-MS

U2 - 10.1016/S0008-6215(01)00327-5

DO - 10.1016/S0008-6215(01)00327-5

M3 - Article

VL - 337

SP - 373

EP - 377

JO - Carbohydrate Research

JF - Carbohydrate Research

SN - 0008-6215

IS - 4

ER -