Characterization of self-assembled protein monomolecular layer and protein-substrate interface

Master's thesis

Research output: ThesisMaster's thesisTheses

Abstract

In this Master's thesis, we studied the self-assembly of a hydrophobin protein named HFBI and the possibility to use directed self-assembly with silicon patterned Si—SiO2 surfaces. The result was investigated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). A clear contrast in protein adhesion and non-adhesion was observed between silicon and silicon dioxide areas, respectively. Additionally, a generic silicon template was designed and fabricated in order to enable electrical characterization of self-assembled hydrophobin monolayers in a controlled configuration setup. Electrical transport measurements were carried out on the final device at room temperature and at cryogenic temperatures (77 K and 4 K).
Original languageEnglish
QualificationMaster Degree
Awarding Institution
  • Helsinki University of Technology
Supervisors/Advisors
  • Kaivola, Matti, Supervisor, External person
  • Kivioja, Jani, Advisor, External person
Place of PublicationEspoo
Publisher
Publication statusPublished - 2009
MoE publication typeG2 Master's thesis, polytechnic Master's thesis

Fingerprint

theses
proteins
self assembly
silicon
cryogenic temperature
adhesion
templates
electron microscopes
microscopes
silicon dioxide
scanning
room temperature
configurations

Keywords

  • hydrophobin monolayer
  • directed self-assembly
  • C-AFM
  • silicon-hydrophobin-interface
  • stencil mask

Cite this

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title = "Characterization of self-assembled protein monomolecular layer and protein-substrate interface: Master's thesis",
abstract = "In this Master's thesis, we studied the self-assembly of a hydrophobin protein named HFBI and the possibility to use directed self-assembly with silicon patterned Si—SiO2 surfaces. The result was investigated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). A clear contrast in protein adhesion and non-adhesion was observed between silicon and silicon dioxide areas, respectively. Additionally, a generic silicon template was designed and fabricated in order to enable electrical characterization of self-assembled hydrophobin monolayers in a controlled configuration setup. Electrical transport measurements were carried out on the final device at room temperature and at cryogenic temperatures (77 K and 4 K).",
keywords = "hydrophobin monolayer, directed self-assembly, C-AFM, silicon-hydrophobin-interface, stencil mask",
author = "Markku Kainlauri",
note = "CA2: TK601 OH: diplomity{\"o} Project code: 27548 PGN: 68 p.",
year = "2009",
language = "English",
publisher = "Helsinki University of Technology",
address = "Finland",
school = "Helsinki University of Technology",

}

Characterization of self-assembled protein monomolecular layer and protein-substrate interface : Master's thesis. / Kainlauri, Markku.

Espoo : Helsinki University of Technology, 2009. 68 p.

Research output: ThesisMaster's thesisTheses

TY - THES

T1 - Characterization of self-assembled protein monomolecular layer and protein-substrate interface

T2 - Master's thesis

AU - Kainlauri, Markku

N1 - CA2: TK601 OH: diplomityö Project code: 27548 PGN: 68 p.

PY - 2009

Y1 - 2009

N2 - In this Master's thesis, we studied the self-assembly of a hydrophobin protein named HFBI and the possibility to use directed self-assembly with silicon patterned Si—SiO2 surfaces. The result was investigated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). A clear contrast in protein adhesion and non-adhesion was observed between silicon and silicon dioxide areas, respectively. Additionally, a generic silicon template was designed and fabricated in order to enable electrical characterization of self-assembled hydrophobin monolayers in a controlled configuration setup. Electrical transport measurements were carried out on the final device at room temperature and at cryogenic temperatures (77 K and 4 K).

AB - In this Master's thesis, we studied the self-assembly of a hydrophobin protein named HFBI and the possibility to use directed self-assembly with silicon patterned Si—SiO2 surfaces. The result was investigated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). A clear contrast in protein adhesion and non-adhesion was observed between silicon and silicon dioxide areas, respectively. Additionally, a generic silicon template was designed and fabricated in order to enable electrical characterization of self-assembled hydrophobin monolayers in a controlled configuration setup. Electrical transport measurements were carried out on the final device at room temperature and at cryogenic temperatures (77 K and 4 K).

KW - hydrophobin monolayer

KW - directed self-assembly

KW - C-AFM

KW - silicon-hydrophobin-interface

KW - stencil mask

M3 - Master's thesis

PB - Helsinki University of Technology

CY - Espoo

ER -