Characterization of self-assembled protein monomolecular layer and protein-substrate interface: Master's thesis

    Research output: ThesisMaster's thesisTheses


    In this Master's thesis, we studied the self-assembly of a hydrophobin protein named HFBI and the possibility to use directed self-assembly with silicon patterned Si—SiO2 surfaces. The result was investigated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). A clear contrast in protein adhesion and non-adhesion was observed between silicon and silicon dioxide areas, respectively. Additionally, a generic silicon template was designed and fabricated in order to enable electrical characterization of self-assembled hydrophobin monolayers in a controlled configuration setup. Electrical transport measurements were carried out on the final device at room temperature and at cryogenic temperatures (77 K and 4 K).
    Original languageEnglish
    QualificationMaster Degree
    Awarding Institution
    • Helsinki University of Technology
    • Kaivola, Matti, Supervisor, External person
    • Kivioja, Jani, Advisor, External person
    Place of PublicationEspoo
    Publication statusPublished - 2009
    MoE publication typeG2 Master's thesis, polytechnic Master's thesis



    • hydrophobin monolayer
    • directed self-assembly
    • C-AFM
    • silicon-hydrophobin-interface
    • stencil mask

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