Characterization of self-assembled protein monomolecular layer and protein-substrate interface: Master's thesis

Markku Kainlauri

Characterization of self-assembled protein monomolecular layer and protein-substrate interface: Master's thesis

Research output: Thesis › Master's thesis

Abstract

In this Master's thesis, we studied the self-assembly of a hydrophobin protein named HFBI and the possibility to use directed self-assembly with silicon patterned Si—SiO2 surfaces. The result was investigated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). A clear contrast in protein adhesion and non-adhesion was observed between silicon and silicon dioxide areas, respectively. Additionally, a generic silicon template was designed and fabricated in order to enable electrical characterization of self-assembled hydrophobin monolayers in a controlled configuration setup. Electrical transport measurements were carried out on the final device at room temperature and at cryogenic temperatures (77 K and 4 K).

Original language	English
Qualification	Master Degree
Awarding Institution	Helsinki University of Technology
Supervisors/Advisors	Kaivola, Matti, Supervisor, External person Kivioja, Jani, Advisor, External person
Place of Publication	Espoo
Publisher	Helsinki University of Technology
Publication status	Published - 2009
MoE publication type	G2 Master's thesis, polytechnic Master's thesis

Keywords

hydrophobin monolayer
directed self-assembly
C-AFM
silicon-hydrophobin-interface
stencil mask

Cite this

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title = "Characterization of self-assembled protein monomolecular layer and protein-substrate interface: Master's thesis",

abstract = "In this Master's thesis, we studied the self-assembly of a hydrophobin protein named HFBI and the possibility to use directed self-assembly with silicon patterned Si—SiO2 surfaces. The result was investigated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). A clear contrast in protein adhesion and non-adhesion was observed between silicon and silicon dioxide areas, respectively. Additionally, a generic silicon template was designed and fabricated in order to enable electrical characterization of self-assembled hydrophobin monolayers in a controlled configuration setup. Electrical transport measurements were carried out on the final device at room temperature and at cryogenic temperatures (77 K and 4 K).",

keywords = "hydrophobin monolayer, directed self-assembly, C-AFM, silicon-hydrophobin-interface, stencil mask",

author = "Markku Kainlauri",

note = "CA2: TK601 OH: diplomity{\"o} Project code: 27548 PGN: 68 p.",

year = "2009",

language = "English",

publisher = "Helsinki University of Technology",

address = "Finland",

school = "Helsinki University of Technology",

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TY - THES

T1 - Characterization of self-assembled protein monomolecular layer and protein-substrate interface

T2 - Master's thesis

AU - Kainlauri, Markku

N1 - CA2: TK601 OH: diplomityö Project code: 27548 PGN: 68 p.

PY - 2009

Y1 - 2009

N2 - In this Master's thesis, we studied the self-assembly of a hydrophobin protein named HFBI and the possibility to use directed self-assembly with silicon patterned Si—SiO2 surfaces. The result was investigated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). A clear contrast in protein adhesion and non-adhesion was observed between silicon and silicon dioxide areas, respectively. Additionally, a generic silicon template was designed and fabricated in order to enable electrical characterization of self-assembled hydrophobin monolayers in a controlled configuration setup. Electrical transport measurements were carried out on the final device at room temperature and at cryogenic temperatures (77 K and 4 K).

AB - In this Master's thesis, we studied the self-assembly of a hydrophobin protein named HFBI and the possibility to use directed self-assembly with silicon patterned Si—SiO2 surfaces. The result was investigated using Scanning Electron Microscope (SEM) and Atomic Force Microscope (AFM). A clear contrast in protein adhesion and non-adhesion was observed between silicon and silicon dioxide areas, respectively. Additionally, a generic silicon template was designed and fabricated in order to enable electrical characterization of self-assembled hydrophobin monolayers in a controlled configuration setup. Electrical transport measurements were carried out on the final device at room temperature and at cryogenic temperatures (77 K and 4 K).

KW - hydrophobin monolayer

KW - directed self-assembly

KW - C-AFM

KW - silicon-hydrophobin-interface

KW - stencil mask

M3 - Master's thesis

PB - Helsinki University of Technology

CY - Espoo

ER -

Characterization of self-assembled protein monomolecular layer and protein-substrate interface: Master's thesis

Abstract

Keywords

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