Characterization of the action of selected essential oil components on gram-negative bacteria

Ilkka Helander, Hanna-Leena Alakomi, Kyösti Latva-Kala, Tiina Mattila-Sandholm, Irene Pol, Eddy Smid, Leon Gorris, Atte von Wright

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Carvacrol, (+)-carvone, thymol, and trans-cinnamaldehyde were tested for their inhibitory activity against Escherichia coli O157:H7 and Salmonella typhimurium. In addition, their toxicity to Photobacterium leiognathi was determined, utilizing a bioluminescence assay.
Their effects on the cell surface were investigated by measuring the uptake of 1-N-phenylnaphthylamine (NPN), by measuring their sensitization of bacterial suspensions toward detergents and lysozyme, and by analyzing material released from cells upon treatment by these agents.
Carvacrol, thymol, and trans-cinnamaldehyde inhibited E. coli and S. typhimurium at 1−3 mM, whereas (+)-carvone was less inhibitory. trans-Cinnamaldehyde was the most inhibitory component toward P. leiognathi. Carvacrol and thymol disintegrated the outer membrane and released outer membrane-associated material from the cells to the external medium; such release by (+)-carvone or trans-cinnamaldehyde was negligible.
Of the tested components, carvacrol and thymol decreased the intracellular ATP pool of E. coli and also inreased extracellular ATP, indicating disruptive action on the cytoplasmic membrane.
Original languageEnglish
Pages (from-to)3590-3595
JournalJournal of Agricultural and Food Chemistry
Volume46
Issue number9
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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Thymol
carvacrol
thymol
Volatile Oils
Gram-Negative Bacteria
Gram-negative bacteria
carvone
Photobacterium leiognathi
Bacteria
essential oils
Escherichia coli
N-phenyl-1-naphthylamine
Salmonella typhimurium
Membranes
Salmonella Typhimurium
Adenosine Triphosphate
Photobacterium
Bioluminescence
Salmonella
Escherichia coli O157

Cite this

Helander, Ilkka ; Alakomi, Hanna-Leena ; Latva-Kala, Kyösti ; Mattila-Sandholm, Tiina ; Pol, Irene ; Smid, Eddy ; Gorris, Leon ; Wright, Atte von. / Characterization of the action of selected essential oil components on gram-negative bacteria. In: Journal of Agricultural and Food Chemistry. 1998 ; Vol. 46, No. 9. pp. 3590-3595.
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title = "Characterization of the action of selected essential oil components on gram-negative bacteria",
abstract = "Carvacrol, (+)-carvone, thymol, and trans-cinnamaldehyde were tested for their inhibitory activity against Escherichia coli O157:H7 and Salmonella typhimurium. In addition, their toxicity to Photobacterium leiognathi was determined, utilizing a bioluminescence assay. Their effects on the cell surface were investigated by measuring the uptake of 1-N-phenylnaphthylamine (NPN), by measuring their sensitization of bacterial suspensions toward detergents and lysozyme, and by analyzing material released from cells upon treatment by these agents. Carvacrol, thymol, and trans-cinnamaldehyde inhibited E. coli and S. typhimurium at 1−3 mM, whereas (+)-carvone was less inhibitory. trans-Cinnamaldehyde was the most inhibitory component toward P. leiognathi. Carvacrol and thymol disintegrated the outer membrane and released outer membrane-associated material from the cells to the external medium; such release by (+)-carvone or trans-cinnamaldehyde was negligible. Of the tested components, carvacrol and thymol decreased the intracellular ATP pool of E. coli and also inreased extracellular ATP, indicating disruptive action on the cytoplasmic membrane.",
author = "Ilkka Helander and Hanna-Leena Alakomi and Ky{\"o}sti Latva-Kala and Tiina Mattila-Sandholm and Irene Pol and Eddy Smid and Leon Gorris and Wright, {Atte von}",
year = "1998",
doi = "10.1021/jf980154m",
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Helander, I, Alakomi, H-L, Latva-Kala, K, Mattila-Sandholm, T, Pol, I, Smid, E, Gorris, L & Wright, AV 1998, 'Characterization of the action of selected essential oil components on gram-negative bacteria', Journal of Agricultural and Food Chemistry, vol. 46, no. 9, pp. 3590-3595. https://doi.org/10.1021/jf980154m

Characterization of the action of selected essential oil components on gram-negative bacteria. / Helander, Ilkka; Alakomi, Hanna-Leena; Latva-Kala, Kyösti; Mattila-Sandholm, Tiina; Pol, Irene; Smid, Eddy; Gorris, Leon; Wright, Atte von.

In: Journal of Agricultural and Food Chemistry, Vol. 46, No. 9, 1998, p. 3590-3595.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of the action of selected essential oil components on gram-negative bacteria

AU - Helander, Ilkka

AU - Alakomi, Hanna-Leena

AU - Latva-Kala, Kyösti

AU - Mattila-Sandholm, Tiina

AU - Pol, Irene

AU - Smid, Eddy

AU - Gorris, Leon

AU - Wright, Atte von

PY - 1998

Y1 - 1998

N2 - Carvacrol, (+)-carvone, thymol, and trans-cinnamaldehyde were tested for their inhibitory activity against Escherichia coli O157:H7 and Salmonella typhimurium. In addition, their toxicity to Photobacterium leiognathi was determined, utilizing a bioluminescence assay. Their effects on the cell surface were investigated by measuring the uptake of 1-N-phenylnaphthylamine (NPN), by measuring their sensitization of bacterial suspensions toward detergents and lysozyme, and by analyzing material released from cells upon treatment by these agents. Carvacrol, thymol, and trans-cinnamaldehyde inhibited E. coli and S. typhimurium at 1−3 mM, whereas (+)-carvone was less inhibitory. trans-Cinnamaldehyde was the most inhibitory component toward P. leiognathi. Carvacrol and thymol disintegrated the outer membrane and released outer membrane-associated material from the cells to the external medium; such release by (+)-carvone or trans-cinnamaldehyde was negligible. Of the tested components, carvacrol and thymol decreased the intracellular ATP pool of E. coli and also inreased extracellular ATP, indicating disruptive action on the cytoplasmic membrane.

AB - Carvacrol, (+)-carvone, thymol, and trans-cinnamaldehyde were tested for their inhibitory activity against Escherichia coli O157:H7 and Salmonella typhimurium. In addition, their toxicity to Photobacterium leiognathi was determined, utilizing a bioluminescence assay. Their effects on the cell surface were investigated by measuring the uptake of 1-N-phenylnaphthylamine (NPN), by measuring their sensitization of bacterial suspensions toward detergents and lysozyme, and by analyzing material released from cells upon treatment by these agents. Carvacrol, thymol, and trans-cinnamaldehyde inhibited E. coli and S. typhimurium at 1−3 mM, whereas (+)-carvone was less inhibitory. trans-Cinnamaldehyde was the most inhibitory component toward P. leiognathi. Carvacrol and thymol disintegrated the outer membrane and released outer membrane-associated material from the cells to the external medium; such release by (+)-carvone or trans-cinnamaldehyde was negligible. Of the tested components, carvacrol and thymol decreased the intracellular ATP pool of E. coli and also inreased extracellular ATP, indicating disruptive action on the cytoplasmic membrane.

U2 - 10.1021/jf980154m

DO - 10.1021/jf980154m

M3 - Article

VL - 46

SP - 3590

EP - 3595

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 9

ER -