Chemical characterization of hydrothermally pretreated and enzyme-digested wheat straw : an evaluation of recalcitrance

Zara Merali, Kaisa Marjamaa, Andres Käsper, Kristiina Kruus, A. Patric Gunning, Vic J. Morris, Keith W. Waldron (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

There is great interest in understanding changes that occur to cell wall constituents during saccharification of pretreated lignocellulose, particularly in relation to recalcitrance of the residues. This paper reports the effects of hydrothermal pretreatment followed by enzyme hydrolysis on the extractability and properties of recalcitrant wheat straw polymers. The results show that the undigested residue had lost much of its archestructure. Compositional analysis portrayed a considerable loss of cross-linking di-ferulic acid phenolics, hemicellulosic and cellulosic sugars. The remaining cellulosic and non-cellulosic polysaccharides were much more readily extractable in alkali and molecular profiling revealed the presence of low Mw oligomers in the fractions suggesting the partial enzyme hydrolysis of hemicelluloses and cellulose. Simultaneous saccharification and fermentation of the pretreated and enzyme-digested residues surprisingly resulted in ethanol yields of up to 99% of the theoretical. This is discussed in relation to the "recalcitrant" nature of the original pretreated and enzyme digested biomass.
Original languageEnglish
Pages (from-to)132-140
JournalFood Chemistry
Volume198
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Straw
wheat straw
Triticum
Saccharification
saccharification
ferulic acid
Enzymes
enzymes
Hydrolysis
hydrolysis
lignocellulose
Alkalies
cell wall components
crosslinking
hemicellulose
Oligomers
alkalis
Cellulose
Sugars
Biomass

Keywords

  • alkali extraction
  • biofuels
  • enzyme saccharification
  • hydrothermal pretreatment
  • wheat straw

Cite this

Merali, Zara ; Marjamaa, Kaisa ; Käsper, Andres ; Kruus, Kristiina ; Gunning, A. Patric ; Morris, Vic J. ; Waldron, Keith W. / Chemical characterization of hydrothermally pretreated and enzyme-digested wheat straw : an evaluation of recalcitrance. In: Food Chemistry. 2016 ; Vol. 198. pp. 132-140.
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abstract = "There is great interest in understanding changes that occur to cell wall constituents during saccharification of pretreated lignocellulose, particularly in relation to recalcitrance of the residues. This paper reports the effects of hydrothermal pretreatment followed by enzyme hydrolysis on the extractability and properties of recalcitrant wheat straw polymers. The results show that the undigested residue had lost much of its archestructure. Compositional analysis portrayed a considerable loss of cross-linking di-ferulic acid phenolics, hemicellulosic and cellulosic sugars. The remaining cellulosic and non-cellulosic polysaccharides were much more readily extractable in alkali and molecular profiling revealed the presence of low Mw oligomers in the fractions suggesting the partial enzyme hydrolysis of hemicelluloses and cellulose. Simultaneous saccharification and fermentation of the pretreated and enzyme-digested residues surprisingly resulted in ethanol yields of up to 99{\%} of the theoretical. This is discussed in relation to the {"}recalcitrant{"} nature of the original pretreated and enzyme digested biomass.",
keywords = "alkali extraction, biofuels, enzyme saccharification, hydrothermal pretreatment, wheat straw",
author = "Zara Merali and Kaisa Marjamaa and Andres K{\"a}sper and Kristiina Kruus and Gunning, {A. Patric} and Morris, {Vic J.} and Waldron, {Keith W.}",
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Chemical characterization of hydrothermally pretreated and enzyme-digested wheat straw : an evaluation of recalcitrance. / Merali, Zara; Marjamaa, Kaisa; Käsper, Andres; Kruus, Kristiina; Gunning, A. Patric; Morris, Vic J.; Waldron, Keith W. (Corresponding Author).

In: Food Chemistry, Vol. 198, 2016, p. 132-140.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Chemical characterization of hydrothermally pretreated and enzyme-digested wheat straw : an evaluation of recalcitrance

AU - Merali, Zara

AU - Marjamaa, Kaisa

AU - Käsper, Andres

AU - Kruus, Kristiina

AU - Gunning, A. Patric

AU - Morris, Vic J.

AU - Waldron, Keith W.

PY - 2016

Y1 - 2016

N2 - There is great interest in understanding changes that occur to cell wall constituents during saccharification of pretreated lignocellulose, particularly in relation to recalcitrance of the residues. This paper reports the effects of hydrothermal pretreatment followed by enzyme hydrolysis on the extractability and properties of recalcitrant wheat straw polymers. The results show that the undigested residue had lost much of its archestructure. Compositional analysis portrayed a considerable loss of cross-linking di-ferulic acid phenolics, hemicellulosic and cellulosic sugars. The remaining cellulosic and non-cellulosic polysaccharides were much more readily extractable in alkali and molecular profiling revealed the presence of low Mw oligomers in the fractions suggesting the partial enzyme hydrolysis of hemicelluloses and cellulose. Simultaneous saccharification and fermentation of the pretreated and enzyme-digested residues surprisingly resulted in ethanol yields of up to 99% of the theoretical. This is discussed in relation to the "recalcitrant" nature of the original pretreated and enzyme digested biomass.

AB - There is great interest in understanding changes that occur to cell wall constituents during saccharification of pretreated lignocellulose, particularly in relation to recalcitrance of the residues. This paper reports the effects of hydrothermal pretreatment followed by enzyme hydrolysis on the extractability and properties of recalcitrant wheat straw polymers. The results show that the undigested residue had lost much of its archestructure. Compositional analysis portrayed a considerable loss of cross-linking di-ferulic acid phenolics, hemicellulosic and cellulosic sugars. The remaining cellulosic and non-cellulosic polysaccharides were much more readily extractable in alkali and molecular profiling revealed the presence of low Mw oligomers in the fractions suggesting the partial enzyme hydrolysis of hemicelluloses and cellulose. Simultaneous saccharification and fermentation of the pretreated and enzyme-digested residues surprisingly resulted in ethanol yields of up to 99% of the theoretical. This is discussed in relation to the "recalcitrant" nature of the original pretreated and enzyme digested biomass.

KW - alkali extraction

KW - biofuels

KW - enzyme saccharification

KW - hydrothermal pretreatment

KW - wheat straw

U2 - 10.1016/j.foodchem.2015.07.108

DO - 10.1016/j.foodchem.2015.07.108

M3 - Article

VL - 198

SP - 132

EP - 140

JO - Food Chemistry

JF - Food Chemistry

SN - 0308-8146

ER -