TY - JOUR
T1 - Chemical profiling and bioactivity of body wall lipids from strongylocentrotus droebachiensis
AU - Shikov, Alexander N.
AU - Laakso, Into
AU - Pozharitskaya, Olga N.
AU - Seppänen-Laakso, Tuulikki
AU - Krishtopina, Anna S.
AU - Makarova, Marina N.
AU - Vuorela, Heikki
AU - Makarov, Valery
N1 - Funding Information:
This study was done without financial support. The cost to publish in open access will be pay from the budget of Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki.
Publisher Copyright:
© 2017 by the authors.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2017/11/24
Y1 - 2017/11/24
N2 - The lipids from gonads and polyhydroxynaphthoquinone pigments from body walls of sea urchins are intensively studied. However, little is known about the body wall (BW) lipids. Ethanol extract (55 ?C) contained about equal amounts of saturated (SaFA) and monounsaturated fatty acids (MUFA) representing 60% of total fatty acids, with myristic, palmitic and eicosenoic acids as major SaFAs and MUFAs, respectively. Non-methylene-interrupted dienes (13%) were composed of eicosadienoic and docosadienoic acids. Long-chain polyunsaturated fatty acids (LC-PUFA) included two main components, n6 arachidonic and n3 eicosapentaenoic acids, even with equal concentrations (15 µg/mg) and a balanced n6/n3 PUFA ratio (0.86). The UPLC-ELSD analysis showed that a great majority of the lipids (80%) in the ethanolic extract were phosphatidylcholine (60 µg/mg) and phosphatidylethanolamine (40 µg/mg), while the proportion of neutral lipids remained lower than 20%. In addition, alkoxyglycerol derivatives—chimyl, selachyl, and batyl alcohols—were quantified. We have assumed that the mechanism of action of body wall lipids in the present study is via the inhibition of MAPK p38, COX-1, and COX-2. Our findings open the prospective to utilize this lipid fraction as a source for the development of drugs with anti-inflammatory activity.
AB - The lipids from gonads and polyhydroxynaphthoquinone pigments from body walls of sea urchins are intensively studied. However, little is known about the body wall (BW) lipids. Ethanol extract (55 ?C) contained about equal amounts of saturated (SaFA) and monounsaturated fatty acids (MUFA) representing 60% of total fatty acids, with myristic, palmitic and eicosenoic acids as major SaFAs and MUFAs, respectively. Non-methylene-interrupted dienes (13%) were composed of eicosadienoic and docosadienoic acids. Long-chain polyunsaturated fatty acids (LC-PUFA) included two main components, n6 arachidonic and n3 eicosapentaenoic acids, even with equal concentrations (15 µg/mg) and a balanced n6/n3 PUFA ratio (0.86). The UPLC-ELSD analysis showed that a great majority of the lipids (80%) in the ethanolic extract were phosphatidylcholine (60 µg/mg) and phosphatidylethanolamine (40 µg/mg), while the proportion of neutral lipids remained lower than 20%. In addition, alkoxyglycerol derivatives—chimyl, selachyl, and batyl alcohols—were quantified. We have assumed that the mechanism of action of body wall lipids in the present study is via the inhibition of MAPK p38, COX-1, and COX-2. Our findings open the prospective to utilize this lipid fraction as a source for the development of drugs with anti-inflammatory activity.
KW - Body wall lipids
KW - COX
KW - GC-MS
KW - Inhibition of p38 MAPK
KW - Sea urchin
KW - UPLC-ELSD
KW - Cyclooxygenase Inhibitors/chemistry
KW - Gas Chromatography-Mass Spectrometry
KW - Strongylocentrotus/chemistry
KW - Lipids/chemistry
KW - Aquatic Organisms
KW - Animals
KW - Cell Line/drug effects
UR - http://www.scopus.com/inward/record.url?scp=85039853344&partnerID=8YFLogxK
U2 - 10.3390/md15120365
DO - 10.3390/md15120365
M3 - Article
AN - SCOPUS:85039853344
SN - 1660-3397
VL - 15
JO - Marine Drugs
JF - Marine Drugs
IS - 12
M1 - 365
ER -