Chemical profiling and bioactivity of body wall lipids from strongylocentrotus droebachiensis

Alexander N. Shikov, Into Laakso, Olga N. Pozharitskaya, Tuulikki Sepp nen-Laakso, Anna S. Krishtopina, Marina N. Makarova, Heikki Vuorela, Valery Makarov

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1 Citation (Scopus)

Abstract

The lipids from gonads and polyhydroxynaphthoquinone pigments from body walls of sea urchins are intensively studied. However, little is known about the body wall (BW) lipids. Ethanol extract (55 ?C) contained about equal amounts of saturated (SaFA) and monounsaturated fatty acids (MUFA) representing 60% of total fatty acids, with myristic, palmitic and eicosenoic acids as major SaFAs and MUFAs, respectively. Non-methylene-interrupted dienes (13%) were composed of eicosadienoic and docosadienoic acids. Long-chain polyunsaturated fatty acids (LC-PUFA) included two main components, n6 arachidonic and n3 eicosapentaenoic acids, even with equal concentrations (15 µg/mg) and a balanced n6/n3 PUFA ratio (0.86). The UPLC-ELSD analysis showed that a great majority of the lipids (80%) in the ethanolic extract were phosphatidylcholine (60 µg/mg) and phosphatidylethanolamine (40 µg/mg), while the proportion of neutral lipids remained lower than 20%. In addition, alkoxyglycerol derivatives—chimyl, selachyl, and batyl alcohols—were quantified. We have assumed that the mechanism of action of body wall lipids in the present study is via the inhibition of MAPK p38, COX-1, and COX-2. Our findings open the prospective to utilize this lipid fraction as a source for the development of drugs with anti-inflammatory activity.

Original languageEnglish
Article number365
JournalMarine Drugs
Volume15
Issue number12
DOIs
Publication statusPublished - 24 Nov 2017
MoE publication typeA1 Journal article-refereed

Keywords

  • Body wall lipids
  • COX
  • GC-MS
  • Inhibition of p38 MAPK
  • Sea urchin
  • UPLC-ELSD
  • Cyclooxygenase Inhibitors/chemistry
  • Gas Chromatography-Mass Spectrometry
  • Strongylocentrotus/chemistry
  • Lipids/chemistry
  • Aquatic Organisms
  • Animals
  • Cell Line/drug effects

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  • Cite this

    Shikov, A. N., Laakso, I., Pozharitskaya, O. N., nen-Laakso, T. S., Krishtopina, A. S., Makarova, M. N., Vuorela, H., & Makarov, V. (2017). Chemical profiling and bioactivity of body wall lipids from strongylocentrotus droebachiensis. Marine Drugs, 15(12), [365]. https://doi.org/10.3390/md15120365