Chromatographic lipid profiling of stress-exposed cells

Susanne K. Wiedmer (Corresponding Author), Alexandra Robciuc, Juhani Kronholm, Juha M. Holopainen, Tuulia Hyötyläinen

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Lipidomics is an emerging field of science not only due to its integral part of cell biology and biophysics but also due to the key role of lipids in the modulation of membrane physical properties, signaling, and cell death regulation. The aim of this study was to characterize changes in N‐palmitoyl ceramide concentration and in the global lipid profile in macrophages challenged by oxidized low‐density lipoprotein and nutrient deprived hepatocytes. For this purpose, a quantitative targeted method based on gas chromatography‐mass spectrometry for the determination of total N‐palmitoyl ceramide concentrations in the cellular membranes of cells under stress was used. Ultrahigh‐performance liquid chromatography‐quadrupole‐time of flight mass spectrometry was applied for the comprehensive profiling of lipids. In essence, we found that both models of cellular stress caused an increase in N‐palmitoyl ceramide levels. In addition, increased levels of other ceramides were observed as well as up‐ and down‐regulation of several other lipid species.
Original languageEnglish
Pages (from-to)1845-1853
JournalJournal of Separation Science
Volume35
Issue number15
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Ceramides
Lipids
Cytology
Biophysics
Membranes
Lipoproteins
Macrophages
Cell death
Spectrometry
Nutrients
Mass spectrometry
Physical properties
Gases
Modulation
Liquids

Keywords

  • Ceramide
  • GC-MS
  • Sphingomyelin
  • Triglycerides
  • UHPLC-MS

Cite this

Wiedmer, S. K., Robciuc, A., Kronholm, J., Holopainen, J. M., & Hyötyläinen, T. (2012). Chromatographic lipid profiling of stress-exposed cells. Journal of Separation Science, 35(15), 1845-1853. https://doi.org/10.1002/jssc.201200252
Wiedmer, Susanne K. ; Robciuc, Alexandra ; Kronholm, Juhani ; Holopainen, Juha M. ; Hyötyläinen, Tuulia. / Chromatographic lipid profiling of stress-exposed cells. In: Journal of Separation Science. 2012 ; Vol. 35, No. 15. pp. 1845-1853.
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Wiedmer, SK, Robciuc, A, Kronholm, J, Holopainen, JM & Hyötyläinen, T 2012, 'Chromatographic lipid profiling of stress-exposed cells', Journal of Separation Science, vol. 35, no. 15, pp. 1845-1853. https://doi.org/10.1002/jssc.201200252

Chromatographic lipid profiling of stress-exposed cells. / Wiedmer, Susanne K. (Corresponding Author); Robciuc, Alexandra; Kronholm, Juhani; Holopainen, Juha M.; Hyötyläinen, Tuulia.

In: Journal of Separation Science, Vol. 35, No. 15, 2012, p. 1845-1853.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Chromatographic lipid profiling of stress-exposed cells

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AU - Robciuc, Alexandra

AU - Kronholm, Juhani

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AU - Hyötyläinen, Tuulia

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AB - Lipidomics is an emerging field of science not only due to its integral part of cell biology and biophysics but also due to the key role of lipids in the modulation of membrane physical properties, signaling, and cell death regulation. The aim of this study was to characterize changes in N‐palmitoyl ceramide concentration and in the global lipid profile in macrophages challenged by oxidized low‐density lipoprotein and nutrient deprived hepatocytes. For this purpose, a quantitative targeted method based on gas chromatography‐mass spectrometry for the determination of total N‐palmitoyl ceramide concentrations in the cellular membranes of cells under stress was used. Ultrahigh‐performance liquid chromatography‐quadrupole‐time of flight mass spectrometry was applied for the comprehensive profiling of lipids. In essence, we found that both models of cellular stress caused an increase in N‐palmitoyl ceramide levels. In addition, increased levels of other ceramides were observed as well as up‐ and down‐regulation of several other lipid species.

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Wiedmer SK, Robciuc A, Kronholm J, Holopainen JM, Hyötyläinen T. Chromatographic lipid profiling of stress-exposed cells. Journal of Separation Science. 2012;35(15):1845-1853. https://doi.org/10.1002/jssc.201200252