Chromosomal integration and expression of two bacterial alfa-acetolactate decarboxylase genes in brewer's yeast

Kristina Blomqvist, Maija-Liisa Suihko, Jonathan Knowles, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

Abstract

A bacterial gene encoding a-acetolactate decarboxylase, isolated from Klebsiella ternigena or Enterobacter
aerogenes, was expressed in brewer's yeast. The genes were expressed under either the yeast phosphoglycerokinase
(PGKI) or the alcohol dehydrogenase (ADHI) promoter and were integrated by gene replacement by
using cotransformation into the PGKI or ADHI locus, respectively, of a brewer's yeast. The expression level
of the aL-acetolactate decarboxylase gene of the PGKI integrant strains was higher than that of the ADHI
integrants. Under pilot-scale brewing conditions, the a-acetolactate decarboxylase activity of the PGKI
integrant strains was sufficient to reduce the formation of diacetyl below the taste threshold value, and no
lagering was needed. The brewing properties of the recombinant yeast strains were otherwise unaltered, and
the quality (most importantly, the flavor) of the trial beers produced was as good as that of the control beer.
Original languageEnglish
Pages (from-to)2796-2803
JournalApplied and Environmental Microbiology
Volume57
Issue number10
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

Fingerprint

acetolactate decarboxylase
brewers yeast
phosphoglycerate kinase
yeast
Saccharomyces cerevisiae
gene
brewing
Taste Threshold
Yeasts
beers
Genes
Diacetyl
Bacterial Genes
Klebsiella
genes
Alcohol Dehydrogenase
taste sensitivity
yeasts
diacetyl
alcohol dehydrogenase

Cite this

@article{ee1ea09dbb3044f8b3a49e4a1057c509,
title = "Chromosomal integration and expression of two bacterial alfa-acetolactate decarboxylase genes in brewer's yeast",
abstract = "A bacterial gene encoding a-acetolactate decarboxylase, isolated from Klebsiella ternigena or Enterobacteraerogenes, was expressed in brewer's yeast. The genes were expressed under either the yeast phosphoglycerokinase(PGKI) or the alcohol dehydrogenase (ADHI) promoter and were integrated by gene replacement byusing cotransformation into the PGKI or ADHI locus, respectively, of a brewer's yeast. The expression levelof the aL-acetolactate decarboxylase gene of the PGKI integrant strains was higher than that of the ADHIintegrants. Under pilot-scale brewing conditions, the a-acetolactate decarboxylase activity of the PGKIintegrant strains was sufficient to reduce the formation of diacetyl below the taste threshold value, and nolagering was needed. The brewing properties of the recombinant yeast strains were otherwise unaltered, andthe quality (most importantly, the flavor) of the trial beers produced was as good as that of the control beer.",
author = "Kristina Blomqvist and Maija-Liisa Suihko and Jonathan Knowles and Merja Penttil{\"a}",
note = "Project code: BIO5008",
year = "1991",
language = "English",
volume = "57",
pages = "2796--2803",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "10",

}

Chromosomal integration and expression of two bacterial alfa-acetolactate decarboxylase genes in brewer's yeast. / Blomqvist, Kristina; Suihko, Maija-Liisa; Knowles, Jonathan; Penttilä, Merja.

In: Applied and Environmental Microbiology, Vol. 57, No. 10, 1991, p. 2796-2803.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Chromosomal integration and expression of two bacterial alfa-acetolactate decarboxylase genes in brewer's yeast

AU - Blomqvist, Kristina

AU - Suihko, Maija-Liisa

AU - Knowles, Jonathan

AU - Penttilä, Merja

N1 - Project code: BIO5008

PY - 1991

Y1 - 1991

N2 - A bacterial gene encoding a-acetolactate decarboxylase, isolated from Klebsiella ternigena or Enterobacteraerogenes, was expressed in brewer's yeast. The genes were expressed under either the yeast phosphoglycerokinase(PGKI) or the alcohol dehydrogenase (ADHI) promoter and were integrated by gene replacement byusing cotransformation into the PGKI or ADHI locus, respectively, of a brewer's yeast. The expression levelof the aL-acetolactate decarboxylase gene of the PGKI integrant strains was higher than that of the ADHIintegrants. Under pilot-scale brewing conditions, the a-acetolactate decarboxylase activity of the PGKIintegrant strains was sufficient to reduce the formation of diacetyl below the taste threshold value, and nolagering was needed. The brewing properties of the recombinant yeast strains were otherwise unaltered, andthe quality (most importantly, the flavor) of the trial beers produced was as good as that of the control beer.

AB - A bacterial gene encoding a-acetolactate decarboxylase, isolated from Klebsiella ternigena or Enterobacteraerogenes, was expressed in brewer's yeast. The genes were expressed under either the yeast phosphoglycerokinase(PGKI) or the alcohol dehydrogenase (ADHI) promoter and were integrated by gene replacement byusing cotransformation into the PGKI or ADHI locus, respectively, of a brewer's yeast. The expression levelof the aL-acetolactate decarboxylase gene of the PGKI integrant strains was higher than that of the ADHIintegrants. Under pilot-scale brewing conditions, the a-acetolactate decarboxylase activity of the PGKIintegrant strains was sufficient to reduce the formation of diacetyl below the taste threshold value, and nolagering was needed. The brewing properties of the recombinant yeast strains were otherwise unaltered, andthe quality (most importantly, the flavor) of the trial beers produced was as good as that of the control beer.

M3 - Article

VL - 57

SP - 2796

EP - 2803

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 10

ER -