Cichorium intybus L. Hairy Roots as a Platform for Antimicrobial Activity

Suvi Häkkinen; Katarina Cankar; Liisa Nohynek; Jeroen van Arkel; Markus Laurel; Kirsi-Marja Oksman-Caldentey; Bart Van Droogenbroeck

doi:10.3390/ph16020140

Cichorium intybus L. Hairy Roots as a Platform for Antimicrobial Activity

Suvi Häkkinen (Corresponding Author), Katarina Cankar, Liisa Nohynek, Jeroen van Arkel, Markus Laurel, Kirsi-Marja Oksman-Caldentey, Bart Van Droogenbroeck

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

Industrial chicory is an important crop for its high dietary fibre content. Besides inulin, chicory taproots contain interesting secondary metabolite compounds, which possess bioactive properties. Hairy roots are differentiated plant cell cultures that have shown to be feasible biotechnological hosts for the production of several plant-derived molecules. In this study, hairy roots of industrial chicory cultivars were established, and their potential as a source of antimicrobial ingredients was assessed. It was shown that hot water extracts of hairy roots possessed antimicrobial activity against relevant human microbes, whereas corresponding chicory taproots did not show activity. Remarkably, a significant antimicrobial activity of hot water extracts of chicory hairy roots towards methicillin-resistant Staphylococcus aureus was observed, indicating a high potential of hairy roots as a host for production of antimicrobial agents.

Original language	English
Article number	140
Number of pages	11
Journal	Pharmaceuticals
Volume	16
Issue number	2
DOIs	https://doi.org/10.3390/ph16020140
Publication status	Published - 18 Feb 2023
MoE publication type	A1 Journal article-refereed

Keywords

chicory
antimicrobial activity
hairy roots
sesquiterpenes
polyphenols
MRSA

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10.3390/ph16020140Licence: CC BY

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title = "Cichorium intybus L. Hairy Roots as a Platform for Antimicrobial Activity",

abstract = "Industrial chicory is an important crop for its high dietary fibre content. Besides inulin, chicory taproots contain interesting secondary metabolite compounds, which possess bioactive properties. Hairy roots are differentiated plant cell cultures that have shown to be feasible biotechnological hosts for the production of several plant-derived molecules. In this study, hairy roots of industrial chicory cultivars were established, and their potential as a source of antimicrobial ingredients was assessed. It was shown that hot water extracts of hairy roots possessed antimicrobial activity against relevant human microbes, whereas corresponding chicory taproots did not show activity. Remarkably, a significant antimicrobial activity of hot water extracts of chicory hairy roots towards methicillin-resistant Staphylococcus aureus was observed, indicating a high potential of hairy roots as a host for production of antimicrobial agents.",

keywords = "chicory, antimicrobial activity, hairy roots, sesquiterpenes, polyphenols, MRSA",

author = "Suvi H{\"a}kkinen and Katarina Cankar and Liisa Nohynek and {van Arkel}, Jeroen and Markus Laurel and Kirsi-Marja Oksman-Caldentey and {Van Droogenbroeck}, Bart",

note = "Funding Information: This work was financially supported by the CichOpt project funded under the ERA-NET FACCE SURPLUS, which received funding from the European Union{\textquoteright}s Horizon 2020 research, innovation programme (Grant Agreement 652615) and EU-H2020 project {\textquoteleft}InnCoCells{\textquoteright} (101000373). Publisher Copyright: {\textcopyright} 2023 by the authors.",

year = "2023",

month = feb,

day = "18",

doi = "10.3390/ph16020140",

language = "English",

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AU - Häkkinen, Suvi

AU - Cankar, Katarina

AU - Nohynek, Liisa

AU - van Arkel, Jeroen

AU - Laurel, Markus

AU - Oksman-Caldentey, Kirsi-Marja

AU - Van Droogenbroeck, Bart

N1 - Funding Information: This work was financially supported by the CichOpt project funded under the ERA-NET FACCE SURPLUS, which received funding from the European Union’s Horizon 2020 research, innovation programme (Grant Agreement 652615) and EU-H2020 project ‘InnCoCells’ (101000373). Publisher Copyright: © 2023 by the authors.

PY - 2023/2/18

Y1 - 2023/2/18

N2 - Industrial chicory is an important crop for its high dietary fibre content. Besides inulin, chicory taproots contain interesting secondary metabolite compounds, which possess bioactive properties. Hairy roots are differentiated plant cell cultures that have shown to be feasible biotechnological hosts for the production of several plant-derived molecules. In this study, hairy roots of industrial chicory cultivars were established, and their potential as a source of antimicrobial ingredients was assessed. It was shown that hot water extracts of hairy roots possessed antimicrobial activity against relevant human microbes, whereas corresponding chicory taproots did not show activity. Remarkably, a significant antimicrobial activity of hot water extracts of chicory hairy roots towards methicillin-resistant Staphylococcus aureus was observed, indicating a high potential of hairy roots as a host for production of antimicrobial agents.

AB - Industrial chicory is an important crop for its high dietary fibre content. Besides inulin, chicory taproots contain interesting secondary metabolite compounds, which possess bioactive properties. Hairy roots are differentiated plant cell cultures that have shown to be feasible biotechnological hosts for the production of several plant-derived molecules. In this study, hairy roots of industrial chicory cultivars were established, and their potential as a source of antimicrobial ingredients was assessed. It was shown that hot water extracts of hairy roots possessed antimicrobial activity against relevant human microbes, whereas corresponding chicory taproots did not show activity. Remarkably, a significant antimicrobial activity of hot water extracts of chicory hairy roots towards methicillin-resistant Staphylococcus aureus was observed, indicating a high potential of hairy roots as a host for production of antimicrobial agents.

KW - chicory

KW - antimicrobial activity

KW - hairy roots

KW - sesquiterpenes

KW - polyphenols

KW - MRSA

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DO - 10.3390/ph16020140

M3 - Article

SN - 1424-8247

VL - 16

JO - Pharmaceuticals

JF - Pharmaceuticals

IS - 2

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ER -

Cichorium intybus L. Hairy Roots as a Platform for Antimicrobial Activity

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Keywords

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