Cloning and sequencing of the yeast Saccharomyces cerevisiae SEC1 gene localized on chromosome IV

Markku Aalto, Laura Ruohonen, Kuniaki Hosono, Sirkka Keränen (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    59 Citations (Scopus)

    Abstract

    The SEC1 gene of yeast Saccharomyces cerevisiae was cloned by complementing the temperature‐sensitive mutantion of sec11 at 37°C, and its nucleotide sequence was determined. SEC1 is a single copy gene and encodes a protein of 724 amino acids and 83,490 daltons with a predicted pI value of 6·11. Hydrophobicity plotting showed no clearly hydrophobic regions suggesting a soluble nature for the protein. Amino acid sequence comparisons revealed no obvious homologies with the proteins in the SWISSPROT databank. Two consensus sequences for the cdc2 encoded protein kinase recognition site were revealed within Sec1p. The codon usage suggests a low expression level for SEC1. The 5′ non‐translated region contains two TATA‐like sequences at ‐52 and ‐215 nucleotides from the translation start site. Two potential regulatory sequences for DNA binding proteins were found in the non‐coding 5′ region: a HAP2/HAP3 consensus recognition sequence at nucleotide ‐154 and a BAF1 consensus recognition sequence at nucleotide ‐136. The SEC1 specific probe detected a 2400 nucleotides long transcript, which was in reasonable agreement with the 2172 nucleotides long open reading frame.

    Original languageEnglish
    Pages (from-to)643 - 650
    Number of pages8
    JournalYeast
    Volume7
    Issue number6
    DOIs
    Publication statusPublished - 1991
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Cloning
    Chromosomes
    Nucleotides
    Yeast
    Saccharomyces cerevisiae
    Organism Cloning
    Genes
    Yeasts
    Consensus Sequence
    Proteins
    Amino acids
    CDC2 Protein Kinase
    DNA-Binding Proteins
    Amino Acids
    Hydrophobic and Hydrophilic Interactions
    Codon
    Protein Kinases
    Open Reading Frames
    Hydrophobicity
    Amino Acid Sequence

    Cite this

    Aalto, Markku ; Ruohonen, Laura ; Hosono, Kuniaki ; Keränen, Sirkka. / Cloning and sequencing of the yeast Saccharomyces cerevisiae SEC1 gene localized on chromosome IV. In: Yeast. 1991 ; Vol. 7, No. 6. pp. 643 - 650.
    @article{60057003789649e7948a3bfd73967265,
    title = "Cloning and sequencing of the yeast Saccharomyces cerevisiae SEC1 gene localized on chromosome IV",
    abstract = "The SEC1 gene of yeast Saccharomyces cerevisiae was cloned by complementing the temperature‐sensitive mutantion of sec1‐1 at 37°C, and its nucleotide sequence was determined. SEC1 is a single copy gene and encodes a protein of 724 amino acids and 83,490 daltons with a predicted pI value of 6·11. Hydrophobicity plotting showed no clearly hydrophobic regions suggesting a soluble nature for the protein. Amino acid sequence comparisons revealed no obvious homologies with the proteins in the SWISSPROT databank. Two consensus sequences for the cdc2 encoded protein kinase recognition site were revealed within Sec1p. The codon usage suggests a low expression level for SEC1. The 5′ non‐translated region contains two TATA‐like sequences at ‐52 and ‐215 nucleotides from the translation start site. Two potential regulatory sequences for DNA binding proteins were found in the non‐coding 5′ region: a HAP2/HAP3 consensus recognition sequence at nucleotide ‐154 and a BAF1 consensus recognition sequence at nucleotide ‐136. The SEC1 specific probe detected a 2400 nucleotides long transcript, which was in reasonable agreement with the 2172 nucleotides long open reading frame.",
    author = "Markku Aalto and Laura Ruohonen and Kuniaki Hosono and Sirkka Ker{\"a}nen",
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    year = "1991",
    doi = "10.1002/yea.320070613",
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    Cloning and sequencing of the yeast Saccharomyces cerevisiae SEC1 gene localized on chromosome IV. / Aalto, Markku; Ruohonen, Laura; Hosono, Kuniaki; Keränen, Sirkka (Corresponding Author).

    In: Yeast, Vol. 7, No. 6, 1991, p. 643 - 650.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Cloning and sequencing of the yeast Saccharomyces cerevisiae SEC1 gene localized on chromosome IV

    AU - Aalto, Markku

    AU - Ruohonen, Laura

    AU - Hosono, Kuniaki

    AU - Keränen, Sirkka

    N1 - Project code: BIO6020

    PY - 1991

    Y1 - 1991

    N2 - The SEC1 gene of yeast Saccharomyces cerevisiae was cloned by complementing the temperature‐sensitive mutantion of sec1‐1 at 37°C, and its nucleotide sequence was determined. SEC1 is a single copy gene and encodes a protein of 724 amino acids and 83,490 daltons with a predicted pI value of 6·11. Hydrophobicity plotting showed no clearly hydrophobic regions suggesting a soluble nature for the protein. Amino acid sequence comparisons revealed no obvious homologies with the proteins in the SWISSPROT databank. Two consensus sequences for the cdc2 encoded protein kinase recognition site were revealed within Sec1p. The codon usage suggests a low expression level for SEC1. The 5′ non‐translated region contains two TATA‐like sequences at ‐52 and ‐215 nucleotides from the translation start site. Two potential regulatory sequences for DNA binding proteins were found in the non‐coding 5′ region: a HAP2/HAP3 consensus recognition sequence at nucleotide ‐154 and a BAF1 consensus recognition sequence at nucleotide ‐136. The SEC1 specific probe detected a 2400 nucleotides long transcript, which was in reasonable agreement with the 2172 nucleotides long open reading frame.

    AB - The SEC1 gene of yeast Saccharomyces cerevisiae was cloned by complementing the temperature‐sensitive mutantion of sec1‐1 at 37°C, and its nucleotide sequence was determined. SEC1 is a single copy gene and encodes a protein of 724 amino acids and 83,490 daltons with a predicted pI value of 6·11. Hydrophobicity plotting showed no clearly hydrophobic regions suggesting a soluble nature for the protein. Amino acid sequence comparisons revealed no obvious homologies with the proteins in the SWISSPROT databank. Two consensus sequences for the cdc2 encoded protein kinase recognition site were revealed within Sec1p. The codon usage suggests a low expression level for SEC1. The 5′ non‐translated region contains two TATA‐like sequences at ‐52 and ‐215 nucleotides from the translation start site. Two potential regulatory sequences for DNA binding proteins were found in the non‐coding 5′ region: a HAP2/HAP3 consensus recognition sequence at nucleotide ‐154 and a BAF1 consensus recognition sequence at nucleotide ‐136. The SEC1 specific probe detected a 2400 nucleotides long transcript, which was in reasonable agreement with the 2172 nucleotides long open reading frame.

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    DO - 10.1002/yea.320070613

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