Cloning, expression, and characterization of novel thermostable family 7 cellobiohydrolases

Sanni Voutilainen, Terhi Puranen, Matti Siika-aho, Arja Lappalainen, Marika Alapuranen, Jarno Kallio, Satu Hooman, Liisa Viikari, Jari Vehmaanperä, Anu Koivula (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    86 Citations (Scopus)

    Abstract

    As part of the effort to find better cellulases for bioethanol production processes, we were looking for novel GH‐7 family cellobiohydrolases, which would be particularly active on insoluble polymeric substrates and participate in the rate‐limiting step in the hydrolysis of cellulose. The enzymatic properties were studied and are reported here for family 7 cellobiohydrolases from the thermophilic fungi Acremonium thermophilum, Thermoascus aurantiacus, and Chaetomium thermophilum. The Trichoderma reesei Cel7A enzyme was used as a reference in the experiments. As the native T. aurantiacus Cel7A has no carbohydrate‐binding module (CBM), recombinant proteins having the CBM from either the C. thermophilum Cel7A or the T. reesei Cel7A were also constructed. All these novel acidic cellobiohydrolases were more thermostable (by 4–10°C) and more active (two‐ to fourfold) in hydrolysis of microcrystalline cellulose (Avicel) at 45°C than T. reesei Cel7A. The C. thermophilum Cel7A showed the highest specific activity and temperature optimum when measured on soluble substrates. The most effective enzyme for Avicel hydrolysis at 70°C, however, was the 2‐module version of the T. aurantiacus Cel7A, which was also relatively weakly inhibited by cellobiose. These results are discussed from the structural point of view based on the three‐dimensional homology models of these enzymes.
    Original languageEnglish
    Pages (from-to)515-528
    Number of pages13
    JournalBiotechnology and Bioengineering
    Volume101
    Issue number3
    DOIs
    Publication statusPublished - 2008
    MoE publication typeA1 Journal article-refereed

    Keywords

    • cellulose
    • cellobiohydrolase
    • Trichoderma reesei
    • Chaetomium thermophilum
    • Acremonium thermophilum
    • Thermoascus aurantiacus

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