Cloning of Aspergillus niger genes in yeast

Expression of the gene coding Aspergillusβ-glucosidase

Meria E. Penttilä, K. M. Helena Nevalainen, Alain Raynal, Jonathan K.C. Knowles

Research output: Contribution to journalArticleScientificpeer-review

49 Citations (Scopus)

Abstract

The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.

Original languageEnglish
Pages (from-to)494-499
Number of pages6
JournalMolecular and General Genetics
Volume194
Issue number3
DOIs
Publication statusPublished - 1 May 1984
MoE publication typeA1 Journal article-refereed

Fingerprint

Glucosidases
Aspergillus niger
Aspergillus
Organism Cloning
Fungal Genes
Yeasts
Gene Expression
Genes
Cosmids
Galactosidases
Glucan 1,4-alpha-Glucosidase
Genetic Vectors
Saccharomyces cerevisiae
Glycoproteins
Fungi
Clone Cells
Genome
Escherichia coli
Mutation
DNA

Cite this

Penttilä, Meria E. ; Helena Nevalainen, K. M. ; Raynal, Alain ; Knowles, Jonathan K.C. / Cloning of Aspergillus niger genes in yeast : Expression of the gene coding Aspergillusβ-glucosidase. In: Molecular and General Genetics. 1984 ; Vol. 194, No. 3. pp. 494-499.
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Cloning of Aspergillus niger genes in yeast : Expression of the gene coding Aspergillusβ-glucosidase. / Penttilä, Meria E.; Helena Nevalainen, K. M.; Raynal, Alain; Knowles, Jonathan K.C.

In: Molecular and General Genetics, Vol. 194, No. 3, 01.05.1984, p. 494-499.

Research output: Contribution to journalArticleScientificpeer-review

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