Cloning of Aspergillus niger genes in yeast: Expression of the gene coding Aspergillus β-glucosidase

Merja E. Penttilä, K. M. Helena Nevalainen, Alain Raynal, Jonathan K.C. Knowles

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    49 Citations (Scopus)

    Abstract

    The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.
    Original languageEnglish
    Pages (from-to)494-499
    JournalMolecular and General Genetics
    Volume194
    Issue number3
    DOIs
    Publication statusPublished - 1 May 1984
    MoE publication typeA1 Journal article-refereed

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