TY - JOUR
T1 - Cloning of Aspergillus niger genes in yeast
T2 - Expression of the gene coding Aspergillus β-glucosidase
AU - Penttilä, Merja E.
AU - Nevalainen, K.M. Helena
AU - Raynal, Alain
AU - Knowles, Jonathan K.C.
PY - 1984/5/1
Y1 - 1984/5/1
N2 - The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.
AB - The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.
UR - http://www.scopus.com/inward/record.url?scp=0021139018&partnerID=8YFLogxK
U2 - 10.1007/BF00425564
DO - 10.1007/BF00425564
M3 - Article
AN - SCOPUS:0021139018
SN - 1617-4615
VL - 194
SP - 494
EP - 499
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 3
ER -