Cloning of Aspergillus niger genes in yeast: Expression of the gene coding Aspergillus β-glucosidase

Merja E. Penttilä; K.M. Helena Nevalainen; Alain Raynal; Jonathan K.C. Knowles

doi:10.1007/BF00425564

Cloning of Aspergillus niger genes in yeast: Expression of the gene coding Aspergillus β-glucosidase

Merja E. Penttilä
, K.M. Helena Nevalainen
, Alain Raynal
, Jonathan K.C. Knowles^*

^*Corresponding author for this work

University of Paris-Saclay

Research output: Contribution to journal › Article › Scientific › peer-review

51 Citations (Scopus)

Abstract

The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.

Original language	English
Pages (from-to)	494-499
Journal	Molecular and General Genetics
Volume	194
Issue number	3
DOIs	https://doi.org/10.1007/BF00425564
Publication status	Published - 1 May 1984
MoE publication type	A1 Journal article-refereed

Access to Document

10.1007/BF00425564

Cite this

@article{963633300e1246caadb7a4b2849cd343,

title = "Cloning of Aspergillus niger genes in yeast: Expression of the gene coding Aspergillus β-glucosidase",

abstract = "The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.",

author = "Penttil{\"a}, \{Merja E.\} and Nevalainen, \{K.M. Helena\} and Alain Raynal and Knowles, \{Jonathan K.C.\}",

year = "1984",

month = may,

day = "1",

doi = "10.1007/BF00425564",

language = "English",

volume = "194",

pages = "494--499",

journal = "Molecular and General Genetics",

issn = "0026-8925",

number = "3",

}

TY - JOUR

T1 - Cloning of Aspergillus niger genes in yeast

T2 - Expression of the gene coding Aspergillus β-glucosidase

AU - Penttilä, Merja E.

AU - Nevalainen, K.M. Helena

AU - Raynal, Alain

AU - Knowles, Jonathan K.C.

PY - 1984/5/1

Y1 - 1984/5/1

N2 - The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.

AB - The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations. Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.

UR - https://www.scopus.com/pages/publications/0021139018

U2 - 10.1007/BF00425564

DO - 10.1007/BF00425564

M3 - Article

AN - SCOPUS:0021139018

SN - 0026-8925

VL - 194

SP - 494

EP - 499

JO - Molecular and General Genetics

JF - Molecular and General Genetics

IS - 3

ER -

Cloning of Aspergillus niger genes in yeast: Expression of the gene coding Aspergillus β-glucosidase

Abstract

Access to Document

Other files and links

Fingerprint

Cite this