Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries

Simo Ellilä (Corresponding Author), Paul Bromann, Mari Nyyssönen, Merja Itävaara, Anu Koivula, Lars Paulin, Kristiina Kruus

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Xylanases are in important class of industrial enzymes that are essential for the complete hydrolysis of lignocellulosic biomass into fermentable sugars. In the present study, we report the cloning of novel xylanases with interesting properties from compost metagenomics libraries. Controlled composting of lignocellulosic materials was used to enrich the microbial population in lignocellulolytic organisms. DNA extracted from the compost samples was used to construct metagenomics libraries, which were screened for xylanase activity. In total, 40 clones exhibiting xylanase activity were identified and the thermostability of the discovered xylanases was assayed directly from the library clones. Five genes, including one belonging to the more rare family GH8, were selected for subcloning and the enzymes were expressed in recombinant form in E. coli. Preliminary characterization of the metagenome-derived xylanases revealed interesting properties of the novel enzymes, such as high thermostability and specific activity, and differences in hydrolysis profiles. One enzyme was found to perform better than a standard Trichoderma reesei xylanase in the hydrolysis of lignocellulose at elevated temperatures.

Original languageEnglish
Article number124
JournalAMB Express
Volume9
Issue number1
DOIs
Publication statusPublished - 2019
MoE publication typeA1 Journal article-refereed

Fingerprint

Metagenomics
Organism Cloning
Soil
Hydrolysis
Enzymes
Clone Cells
Metagenome
Trichoderma
Biomass
Libraries
Escherichia coli
Temperature
lignocellulose
DNA
Population
Genes

Keywords

  • Cloning
  • Compost
  • Lignocellulose
  • Metagenomics
  • Screening
  • Xylanase

Cite this

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title = "Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries",
abstract = "Xylanases are in important class of industrial enzymes that are essential for the complete hydrolysis of lignocellulosic biomass into fermentable sugars. In the present study, we report the cloning of novel xylanases with interesting properties from compost metagenomics libraries. Controlled composting of lignocellulosic materials was used to enrich the microbial population in lignocellulolytic organisms. DNA extracted from the compost samples was used to construct metagenomics libraries, which were screened for xylanase activity. In total, 40 clones exhibiting xylanase activity were identified and the thermostability of the discovered xylanases was assayed directly from the library clones. Five genes, including one belonging to the more rare family GH8, were selected for subcloning and the enzymes were expressed in recombinant form in E. coli. Preliminary characterization of the metagenome-derived xylanases revealed interesting properties of the novel enzymes, such as high thermostability and specific activity, and differences in hydrolysis profiles. One enzyme was found to perform better than a standard Trichoderma reesei xylanase in the hydrolysis of lignocellulose at elevated temperatures.",
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Cloning of novel bacterial xylanases from lignocellulose-enriched compost metagenomic libraries. / Ellilä, Simo (Corresponding Author); Bromann, Paul; Nyyssönen, Mari; Itävaara, Merja; Koivula, Anu; Paulin, Lars; Kruus, Kristiina.

In: AMB Express, Vol. 9, No. 1, 124, 2019.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Bromann, Paul

AU - Nyyssönen, Mari

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AU - Koivula, Anu

AU - Paulin, Lars

AU - Kruus, Kristiina

PY - 2019

Y1 - 2019

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