Cloning of two genes (LAT1,2) encoding specific l-arabinose transporters of the l-arabinose fermenting yeast ambrosiozyma monospora

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    Abstract

    We identified and characterized two genes, LAT1 and LAT2, which encode specific l-arabinose transporters. The genes were identified in the l-arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low l-arabinose transport activity; however, when LAT1 or LAT2 was expressed, l-arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the l-arabinose transporters could not restore growth on d-glucose, d-fructose, d-mannose or d-galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for l-arabinose.

    Original languageEnglish
    Pages (from-to)604-611
    Number of pages8
    JournalApplied Biochemistry and Biotechnology
    Volume164
    Issue number5
    DOIs
    Publication statusPublished - 1 Jul 2011
    MoE publication typeA1 Journal article-refereed

    Keywords

    • l-Arabinose metabolism
    • l-Arabinose transport
    • Metabolic engineering
    • Pentose fermentation
    • Sugar transport
    • Yeast

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