Cloning of two genes (LAT1,2) encoding specific l-arabinose transporters of the l-arabinose fermenting yeast ambrosiozyma monospora

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

We identified and characterized two genes, LAT1 and LAT2, which encode specific l-arabinose transporters. The genes were identified in the l-arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low l-arabinose transport activity; however, when LAT1 or LAT2 was expressed, l-arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the l-arabinose transporters could not restore growth on d-glucose, d-fructose, d-mannose or d-galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for l-arabinose.

Original languageEnglish
Pages (from-to)604-611
Number of pages8
JournalApplied Biochemistry and Biotechnology
Volume164
Issue number5
DOIs
Publication statusPublished - 1 Jul 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Arabinose
Cloning
Yeast
Organism Cloning
Genes
Yeasts
Fructose
Saccharomyces cerevisiae
Monosaccharide Transport Proteins
Sugars
Glucose
Mannose
Galactose
Growth

Keywords

  • l-Arabinose metabolism
  • l-Arabinose transport
  • Metabolic engineering
  • Pentose fermentation
  • Sugar transport
  • Yeast

Cite this

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title = "Cloning of two genes (LAT1,2) encoding specific l-arabinose transporters of the l-arabinose fermenting yeast ambrosiozyma monospora",
abstract = "We identified and characterized two genes, LAT1 and LAT2, which encode specific l-arabinose transporters. The genes were identified in the l-arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low l-arabinose transport activity; however, when LAT1 or LAT2 was expressed, l-arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the l-arabinose transporters could not restore growth on d-glucose, d-fructose, d-mannose or d-galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for l-arabinose.",
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Cloning of two genes (LAT1,2) encoding specific l-arabinose transporters of the l-arabinose fermenting yeast ambrosiozyma monospora. / Verho, Ritva; Penttilä, Merja; Richard, Peter.

In: Applied Biochemistry and Biotechnology, Vol. 164, No. 5, 01.07.2011, p. 604-611.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Cloning of two genes (LAT1,2) encoding specific l-arabinose transporters of the l-arabinose fermenting yeast ambrosiozyma monospora

AU - Verho, Ritva

AU - Penttilä, Merja

AU - Richard, Peter

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AB - We identified and characterized two genes, LAT1 and LAT2, which encode specific l-arabinose transporters. The genes were identified in the l-arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low l-arabinose transport activity; however, when LAT1 or LAT2 was expressed, l-arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the l-arabinose transporters could not restore growth on d-glucose, d-fructose, d-mannose or d-galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for l-arabinose.

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