Abstract
We identified and characterized two genes, LAT1 and LAT2, which encode specific l-arabinose transporters. The genes were identified in the l-arabinose fermenting yeast Ambrosiozyma monospora. The yeast Saccharomyces cerevisiae had only very low l-arabinose transport activity; however, when LAT1 or LAT2 was expressed, l-arabinose transport was facilitated. When the LAT1 or LAT2 were expressed in an S. cerevisiae mutant where the main hexose transporters were deleted, the l-arabinose transporters could not restore growth on d-glucose, d-fructose, d-mannose or d-galactose. This indicates that these sugars are not transported and suggests that the transporters are specific for l-arabinose.
| Original language | English |
|---|---|
| Pages (from-to) | 604-611 |
| Number of pages | 8 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume | 164 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 1 Jul 2011 |
| MoE publication type | A1 Journal article-refereed |
Funding
Acknowledgment This work was supported by the Academy of Finland through the following programmes: an Academy Research Fellowship for P.R. and the Finnish Centre of Excellence in White Biotechnology—Green Chemistry.
Keywords
- l-Arabinose metabolism
- l-Arabinose transport
- Metabolic engineering
- Pentose fermentation
- Sugar transport
- Yeast