Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Deborah M. Waters; Patrick G. Murray; Yuta Miki; Angel T. Martínez; Maria G. Tuohy; Craig Faulds

doi:10.1128/AEM.05659-11

Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Deborah M. Waters, Patrick G. Murray, Yuta Miki, Angel T. Martínez, Maria G. Tuohy, Craig Faulds

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

8 Citations (Scopus)

Abstract

The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

Original language	English
Pages (from-to)	3759-3762
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	10
DOIs	https://doi.org/10.1128/AEM.05659-11
Publication status	Published - 2012
MoE publication type	A1 Journal article-refereed

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10.1128/AEM.05659-11

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title = "Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii",

abstract = "The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.",

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AU - Waters, Deborah M.

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AU - Martínez, Angel T.

AU - Tuohy, Maria G.

AU - Faulds, Craig

PY - 2012

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AB - The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

U2 - 10.1128/AEM.05659-11

DO - 10.1128/AEM.05659-11

M3 - Article

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 10

ER -

Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Abstract

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