Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Deborah M. Waters, Patrick G. Murray, Yuta Miki, Angel T. Martínez, Maria G. Tuohy, Craig Faulds

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.
Original languageEnglish
Pages (from-to)3759-3762
JournalApplied and Environmental Microbiology
Volume78
Issue number10
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

acetylxylan esterase
Talaromyces
Ascomycota
Biofuels
Esterases
biofuel
esterases
Biomass
Organism Cloning
molecular cloning
Escherichia coli
Temperature
gene
biomass
biotransformation
thermal stability
biofuels
Genes
temperature
genes

Cite this

Waters, Deborah M. ; Murray, Patrick G. ; Miki, Yuta ; Martínez, Angel T. ; Tuohy, Maria G. ; Faulds, Craig. / Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii. In: Applied and Environmental Microbiology. 2012 ; Vol. 78, No. 10. pp. 3759-3762.
@article{245207ddc99c4a43a201747cd57c1d96,
title = "Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii",
abstract = "The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.",
author = "Waters, {Deborah M.} and Murray, {Patrick G.} and Yuta Miki and Mart{\'i}nez, {Angel T.} and Tuohy, {Maria G.} and Craig Faulds",
year = "2012",
doi = "10.1128/AEM.05659-11",
language = "English",
volume = "78",
pages = "3759--3762",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "10",

}

Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii. / Waters, Deborah M.; Murray, Patrick G.; Miki, Yuta; Martínez, Angel T.; Tuohy, Maria G.; Faulds, Craig.

In: Applied and Environmental Microbiology, Vol. 78, No. 10, 2012, p. 3759-3762.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

AU - Waters, Deborah M.

AU - Murray, Patrick G.

AU - Miki, Yuta

AU - Martínez, Angel T.

AU - Tuohy, Maria G.

AU - Faulds, Craig

PY - 2012

Y1 - 2012

N2 - The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

AB - The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

U2 - 10.1128/AEM.05659-11

DO - 10.1128/AEM.05659-11

M3 - Article

VL - 78

SP - 3759

EP - 3762

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 10

ER -