Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Deborah M. Waters; Patrick G. Murray; Yuta Miki; Angel T. Martínez; Maria G. Tuohy; Craig Faulds

doi:10.1128/AEM.05659-11

Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Deborah M. Waters
, Patrick G. Murray
, Yuta Miki
, Angel T. Martínez
, Maria G. Tuohy
, Craig Faulds

VTT Technical Research Centre of Finland

National University of Ireland
Limerick Institute of Technology
Spanish National Research Council (CSIC)
VTT (former employee or external)

Research output: Contribution to journal › Article › Scientific › peer-review

10 Citations (Scopus)

Abstract

The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

Original language	English
Pages (from-to)	3759-3762
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	10
DOIs	https://doi.org/10.1128/AEM.05659-11
Publication status	Published - 2012
MoE publication type	A1 Journal article-refereed

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

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10.1128/AEM.05659-11

Cite this

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title = "Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii",

abstract = "The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.",

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pages = "3759--3762",

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AU - Waters, Deborah M.

AU - Murray, Patrick G.

AU - Miki, Yuta

AU - Martínez, Angel T.

AU - Tuohy, Maria G.

AU - Faulds, Craig

PY - 2012

Y1 - 2012

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AB - The gene encoding an acetylxylan esterase (AXE1) from the thermophilic ascomycete Talaromyces emersonii was cloned, expressed in Escherichia coli, and characterized. This form of AXE1, rTeAXE1, exhibits increased thermostability and activity at a higher temperature than other known fungal acetyl esterases, thus having huge potential application in biomass bioconversion to high value chemicals or biofuels.

U2 - 10.1128/AEM.05659-11

DO - 10.1128/AEM.05659-11

M3 - Article

SN - 0099-2240

VL - 78

SP - 3759

EP - 3762

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 10

ER -

Cloning, overexpression in Escherichia coli, and characterization of a thermostable fungal acetylxylan esterase from Talaromyces emersonii

Abstract

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