CMC-modified cellulose biointerface for antibody conjugation

Hannes Orelma (Corresponding Author), Tuija Teerinen, Leena-Sisko Johansson, Susanna Holappa, Janne Laine

    Research output: Contribution to journalArticleScientificpeer-review

    96 Citations (Scopus)


    In this Article, we present a new strategy for preparing an antihemoglobin biointerface on cellulose. The preparation method is based on functionalization of the cellulose surface by the irreversible adsorption of CMC, followed by covalent linking of antibodies to CMC. This would provide the means for affordable and stable cellulose-based biointerfaces for immunoassays. The preparation and characterization of the biointerface were studied on Langmuir–Schaefer cellulose model surfaces in real time using the quartz crystal microbalance with dissipation and surface plasmon resonance techniques. The stable attachment of antihemoglobin to adsorbed CMC was achieved, and a linear calibration of hemoglobin was obtained. CMC modification was also observed to prevent nonspecific protein adsorption. The antihemoglobin-CMC surface regenerated well, enabling repeated immunodetection cycles of hemoglobin on the same surface.
    Original languageEnglish
    Pages (from-to)1051-1058
    Issue number4
    Publication statusPublished - 2012
    MoE publication typeA1 Journal article-refereed


    This research was supported by the National Technology Agency of Finland (TEKES) (project no. 534-E5SU00763 Bioactive paper).


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