CMC-modified cellulose biointerface for antibody conjugation

Hannes Orelma (Corresponding Author), Tuija Teerinen, Leena-Sisko Johansson, Susanna Holappa, Janne Laine

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

In this Article, we present a new strategy for preparing an antihemoglobin biointerface on cellulose. The preparation method is based on functionalization of the cellulose surface by the irreversible adsorption of CMC, followed by covalent linking of antibodies to CMC. This would provide the means for affordable and stable cellulose-based biointerfaces for immunoassays. The preparation and characterization of the biointerface were studied on Langmuir–Schaefer cellulose model surfaces in real time using the quartz crystal microbalance with dissipation and surface plasmon resonance techniques. The stable attachment of antihemoglobin to adsorbed CMC was achieved, and a linear calibration of hemoglobin was obtained. CMC modification was also observed to prevent nonspecific protein adsorption. The antihemoglobin-CMC surface regenerated well, enabling repeated immunodetection cycles of hemoglobin on the same surface.
Original languageEnglish
Pages (from-to)1051-1058
Number of pages8
JournalBiomacromolecules
Volume13
Issue number4
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

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Antibodies
Cellulose
Hemoglobin
Hemoglobins
Adsorption
Quartz crystal microbalances
Surface plasmon resonance
Calibration
Proteins

Cite this

Orelma, Hannes ; Teerinen, Tuija ; Johansson, Leena-Sisko ; Holappa, Susanna ; Laine, Janne. / CMC-modified cellulose biointerface for antibody conjugation. In: Biomacromolecules. 2012 ; Vol. 13, No. 4. pp. 1051-1058.
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CMC-modified cellulose biointerface for antibody conjugation. / Orelma, Hannes (Corresponding Author); Teerinen, Tuija; Johansson, Leena-Sisko; Holappa, Susanna; Laine, Janne.

In: Biomacromolecules, Vol. 13, No. 4, 2012, p. 1051-1058.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - CMC-modified cellulose biointerface for antibody conjugation

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AU - Holappa, Susanna

AU - Laine, Janne

PY - 2012

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AB - In this Article, we present a new strategy for preparing an antihemoglobin biointerface on cellulose. The preparation method is based on functionalization of the cellulose surface by the irreversible adsorption of CMC, followed by covalent linking of antibodies to CMC. This would provide the means for affordable and stable cellulose-based biointerfaces for immunoassays. The preparation and characterization of the biointerface were studied on Langmuir–Schaefer cellulose model surfaces in real time using the quartz crystal microbalance with dissipation and surface plasmon resonance techniques. The stable attachment of antihemoglobin to adsorbed CMC was achieved, and a linear calibration of hemoglobin was obtained. CMC modification was also observed to prevent nonspecific protein adsorption. The antihemoglobin-CMC surface regenerated well, enabling repeated immunodetection cycles of hemoglobin on the same surface.

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