Coacervation of resilin fusion proteins containing terminal functionalities

Wenwen Fang, Nonappa, Marika Vitikainen, Pezhman Mohammadi, Salla Koskela, Miika Soikkeli, Ann Westerholm-Parvinen, Christopher P. Landowski, Merja Penttilä, Markus B. Linder, Päivi Laaksonen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.

Original languageEnglish
Pages (from-to)590-596
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume171
DOIs
Publication statusPublished - 1 Nov 2018
MoE publication typeNot Eligible

Fingerprint

Fusion reactions
fusion
proteins
Proteins
Peptides
peptides
Electron Microscope Tomography
Graphite
Phase Transition
Liquids
cellulose
Particle Size
Cellulose
Cryogenics
Graphene
cryogenics
Tomography
graphene
liquid phases
adhesion

Keywords

  • Coacervation
  • Resilin
  • Selective adhesion
  • Self-assembly
  • Tomography

Cite this

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title = "Coacervation of resilin fusion proteins containing terminal functionalities",
abstract = "Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.",
keywords = "Coacervation, Resilin, Selective adhesion, Self-assembly, Tomography",
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doi = "10.1016/j.colsurfb.2018.07.048",
language = "English",
volume = "171",
pages = "590--596",
journal = "Colloids and Surfaces B: Biointerfaces",
issn = "0927-7765",
publisher = "Elsevier",

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Coacervation of resilin fusion proteins containing terminal functionalities. / Fang, Wenwen; Nonappa, ; Vitikainen, Marika; Mohammadi, Pezhman; Koskela, Salla; Soikkeli, Miika; Westerholm-Parvinen, Ann; Landowski, Christopher P.; Penttilä, Merja; Linder, Markus B.; Laaksonen, Päivi (Corresponding Author).

In: Colloids and Surfaces B: Biointerfaces, Vol. 171, 01.11.2018, p. 590-596.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Coacervation of resilin fusion proteins containing terminal functionalities

AU - Fang, Wenwen

AU - Nonappa,

AU - Vitikainen, Marika

AU - Mohammadi, Pezhman

AU - Koskela, Salla

AU - Soikkeli, Miika

AU - Westerholm-Parvinen, Ann

AU - Landowski, Christopher P.

AU - Penttilä, Merja

AU - Linder, Markus B.

AU - Laaksonen, Päivi

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AB - Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.

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KW - Resilin

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