Coacervation of resilin fusion proteins containing terminal functionalities

Wenwen Fang, Nonappa, Marika Vitikainen, Pezhman Mohammadi, Salla Koskela, Miika Soikkeli, Ann Westerholm-Parvinen, Christopher P. Landowski, Merja Penttilä, Markus B. Linder, Päivi Laaksonen (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.

Original languageEnglish
Pages (from-to)590-596
Number of pages7
JournalColloids and Surfaces B: Biointerfaces
Volume171
DOIs
Publication statusPublished - 1 Nov 2018
MoE publication typeA1 Journal article-refereed

Fingerprint

Fusion reactions
fusion
proteins
Proteins
Peptides
peptides
Electron Microscope Tomography
Graphite
Phase Transition
Liquids
cellulose
Particle Size
Cellulose
Cryogenics
Graphene
cryogenics
Tomography
graphene
liquid phases
adhesion

Keywords

  • Coacervation
  • Resilin
  • Selective adhesion
  • Self-assembly
  • Tomography

Cite this

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title = "Coacervation of resilin fusion proteins containing terminal functionalities",
abstract = "Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.",
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Coacervation of resilin fusion proteins containing terminal functionalities. / Fang, Wenwen; Nonappa, ; Vitikainen, Marika; Mohammadi, Pezhman; Koskela, Salla; Soikkeli, Miika; Westerholm-Parvinen, Ann; Landowski, Christopher P.; Penttilä, Merja; Linder, Markus B.; Laaksonen, Päivi (Corresponding Author).

In: Colloids and Surfaces B: Biointerfaces, Vol. 171, 01.11.2018, p. 590-596.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Coacervation of resilin fusion proteins containing terminal functionalities

AU - Fang, Wenwen

AU - Nonappa,

AU - Vitikainen, Marika

AU - Mohammadi, Pezhman

AU - Koskela, Salla

AU - Soikkeli, Miika

AU - Westerholm-Parvinen, Ann

AU - Landowski, Christopher P.

AU - Penttilä, Merja

AU - Linder, Markus B.

AU - Laaksonen, Päivi

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AB - Liquid-liquid phase transition known as coacervation of resilin-like-peptide fusion proteins containing different terminal domains were investigated. Two different modular proteins were designed and produced and their behavior were compared to a resilin-like-peptide without terminal domains. The size of the particle-like coacervates was modulated by the protein concentration, pH and temperature. The morphology and three-dimensional (3D) structural details of the coacervate particles were investigated by cryogenic transmission electron microscopy (cryo-TEM) and tomography (cryo-ET) reconstruction. Selective adhesion of the coacervates on cellulose and graphene surfaces was demonstrated.

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