Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells

Lauri J. Reuter, Mohammad-Ali Shahbazi, Ermei M. Mäkilä, Jarno J. Salonen, Reza Saberianfar, Rima Menassa, Hélder A. Santos, Jussi J. Joensuu, Anneli Ritala

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

The encapsulation of drugs to nanoparticles may offer a solution for targeted delivery. Here, we set out to engineer a self-assembling targeting ligand by combining the functional properties of human transferrin and fungal hydrophobins in a single fusion protein. We showed that human transferrin can be expressed in Nicotiana benthamiana plants as a fusion with Trichoderma reesei hydrophobins HFBI, HFBII, or HFBIV. Transferrin-HFBIV was further expressed in tobacco BY-2 suspension cells. Both partners of the fusion protein retained their functionality; the hydrophobin moiety enabled migration to a surfactant phase in an aqueous two-phase system, and the transferrin moiety was able to reversibly bind iron. Coating porous silicon nanoparticles with the fusion protein resulted in uptake of the nanoparticles in human cancer cells. This study provides a proof-of-concept for the functionalization of hydrophobin coatings with transferrin as a targeting ligand.

Original languageEnglish
Pages (from-to)1639-1648
Number of pages10
JournalBioconjugate Chemistry
Volume28
Issue number6
DOIs
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Transferrin
Nanoparticles
Fusion reactions
Cells
Proteins
Coatings
Neoplasms
Ligands
Tobacco
Porous silicon
Encapsulation
Trichoderma
Silicon
Surface active agents
Surface-Active Agents
Iron
Suspensions
Engineers
Pharmaceutical Preparations

Cite this

Reuter, Lauri J. ; Shahbazi, Mohammad-Ali ; Mäkilä, Ermei M. ; Salonen, Jarno J. ; Saberianfar, Reza ; Menassa, Rima ; Santos, Hélder A. ; Joensuu, Jussi J. ; Ritala, Anneli. / Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells. In: Bioconjugate Chemistry. 2017 ; Vol. 28, No. 6. pp. 1639-1648.
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abstract = "The encapsulation of drugs to nanoparticles may offer a solution for targeted delivery. Here, we set out to engineer a self-assembling targeting ligand by combining the functional properties of human transferrin and fungal hydrophobins in a single fusion protein. We showed that human transferrin can be expressed in Nicotiana benthamiana plants as a fusion with Trichoderma reesei hydrophobins HFBI, HFBII, or HFBIV. Transferrin-HFBIV was further expressed in tobacco BY-2 suspension cells. Both partners of the fusion protein retained their functionality; the hydrophobin moiety enabled migration to a surfactant phase in an aqueous two-phase system, and the transferrin moiety was able to reversibly bind iron. Coating porous silicon nanoparticles with the fusion protein resulted in uptake of the nanoparticles in human cancer cells. This study provides a proof-of-concept for the functionalization of hydrophobin coatings with transferrin as a targeting ligand.",
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Coating Nanoparticles with Plant-Produced Transferrin-Hydrophobin Fusion Protein Enhances Their Uptake in Cancer Cells. / Reuter, Lauri J.; Shahbazi, Mohammad-Ali; Mäkilä, Ermei M.; Salonen, Jarno J.; Saberianfar, Reza; Menassa, Rima; Santos, Hélder A.; Joensuu, Jussi J.; Ritala, Anneli.

In: Bioconjugate Chemistry, Vol. 28, No. 6, 2017, p. 1639-1648.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Salonen, Jarno J.

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