Combination of three techniques for the study of complex enzyme mixtures: Polyacrylamide gel electrophoresis, immunodiffusion and detection of enzymatic activity

Martti Nummi, Arja Lappalainen

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

A technique is presented in which two different separation methods are combined. The first separation is carried out in polyacrylamide gel electrophoresis, which in then followed by immunodiffusion in agarose against appropriate antisera. Soluble and insoluble macromolecular carbohydrates were used as substrates in the detection of the various enzymatic activities. These three methods in combination can provide new information of enzymatically active proteins in mixtures, such as isoenzymes, and multiple forms varying in molecular size.

Original languageEnglish
Pages (from-to)295 - 302
Number of pages8
JournalJournal of Biochemical and Biophysical Methods
Volume11
Issue number4-5
DOIs
Publication statusPublished - 1985
MoE publication typeNot Eligible

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Immunodiffusion
Electrophoresis
Complex Mixtures
Polyacrylamide Gel Electrophoresis
Enzymes
Sepharose
Isoenzymes
Immune Sera
Carbohydrates
Substrates
Proteins
polyacrylamide gels

Cite this

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title = "Combination of three techniques for the study of complex enzyme mixtures: Polyacrylamide gel electrophoresis, immunodiffusion and detection of enzymatic activity",
abstract = "A technique is presented in which two different separation methods are combined. The first separation is carried out in polyacrylamide gel electrophoresis, which in then followed by immunodiffusion in agarose against appropriate antisera. Soluble and insoluble macromolecular carbohydrates were used as substrates in the detection of the various enzymatic activities. These three methods in combination can provide new information of enzymatically active proteins in mixtures, such as isoenzymes, and multiple forms varying in molecular size.",
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Combination of three techniques for the study of complex enzyme mixtures : Polyacrylamide gel electrophoresis, immunodiffusion and detection of enzymatic activity. / Nummi, Martti; Lappalainen, Arja.

In: Journal of Biochemical and Biophysical Methods, Vol. 11, No. 4-5, 1985, p. 295 - 302.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Combination of three techniques for the study of complex enzyme mixtures

T2 - Polyacrylamide gel electrophoresis, immunodiffusion and detection of enzymatic activity

AU - Nummi, Martti

AU - Lappalainen, Arja

PY - 1985

Y1 - 1985

N2 - A technique is presented in which two different separation methods are combined. The first separation is carried out in polyacrylamide gel electrophoresis, which in then followed by immunodiffusion in agarose against appropriate antisera. Soluble and insoluble macromolecular carbohydrates were used as substrates in the detection of the various enzymatic activities. These three methods in combination can provide new information of enzymatically active proteins in mixtures, such as isoenzymes, and multiple forms varying in molecular size.

AB - A technique is presented in which two different separation methods are combined. The first separation is carried out in polyacrylamide gel electrophoresis, which in then followed by immunodiffusion in agarose against appropriate antisera. Soluble and insoluble macromolecular carbohydrates were used as substrates in the detection of the various enzymatic activities. These three methods in combination can provide new information of enzymatically active proteins in mixtures, such as isoenzymes, and multiple forms varying in molecular size.

U2 - 10.1016/0165-022X(85)90011-9

DO - 10.1016/0165-022X(85)90011-9

M3 - Article

VL - 11

SP - 295

EP - 302

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 4-5

ER -