Abstract
A technique is presented in which two different separation methods are combined. The first separation is carried out in polyacrylamide gel electrophoresis, which in then followed by immunodiffusion in agarose against appropriate antisera. Soluble and insoluble macromolecular carbohydrates were used as substrates in the detection of the various enzymatic activities. These three methods in combination can provide new information of enzymatically active proteins in mixtures, such as isoenzymes, and multiple forms varying in molecular size.
| Original language | English |
|---|---|
| Pages (from-to) | 295 - 302 |
| Number of pages | 8 |
| Journal | Journal of Biochemical and Biophysical Methods |
| Volume | 11 |
| Issue number | 4-5 |
| DOIs | |
| Publication status | Published - 1985 |
| MoE publication type | Not Eligible |
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Combination of three techniques for the study of complex enzyme mixtures : Polyacrylamide gel electrophoresis, immunodiffusion and detection of enzymatic activity. / Nummi, Martti; Lappalainen, Arja.
In: Journal of Biochemical and Biophysical Methods, Vol. 11, No. 4-5, 1985, p. 295 - 302.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Combination of three techniques for the study of complex enzyme mixtures
T2 - Polyacrylamide gel electrophoresis, immunodiffusion and detection of enzymatic activity
AU - Nummi, Martti
AU - Lappalainen, Arja
PY - 1985
Y1 - 1985
N2 - A technique is presented in which two different separation methods are combined. The first separation is carried out in polyacrylamide gel electrophoresis, which in then followed by immunodiffusion in agarose against appropriate antisera. Soluble and insoluble macromolecular carbohydrates were used as substrates in the detection of the various enzymatic activities. These three methods in combination can provide new information of enzymatically active proteins in mixtures, such as isoenzymes, and multiple forms varying in molecular size.
AB - A technique is presented in which two different separation methods are combined. The first separation is carried out in polyacrylamide gel electrophoresis, which in then followed by immunodiffusion in agarose against appropriate antisera. Soluble and insoluble macromolecular carbohydrates were used as substrates in the detection of the various enzymatic activities. These three methods in combination can provide new information of enzymatically active proteins in mixtures, such as isoenzymes, and multiple forms varying in molecular size.
U2 - 10.1016/0165-022X(85)90011-9
DO - 10.1016/0165-022X(85)90011-9
M3 - Article
VL - 11
SP - 295
EP - 302
JO - Journal of Proteomics
JF - Journal of Proteomics
SN - 1874-3919
IS - 4-5
ER -