Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae

Mikko Arvas (Corresponding Author), Tiina Pakula, Karin Lanthaler, Markku Saloheimo, Mari Valkonen, Tapani Suortti, Geoff Robson, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

75 Citations (Scopus)

Abstract

Background: Secretion stress is caused by compromised folding, modification or transport of proteins in the secretory pathway. In fungi, induction of genes in response to secretion stress is mediated mainly by the unfolded protein response (UPR) pathway. This study aims at uncovering transcriptional responses occurring in the filamentous fungi Trichoderma reesei exposed to secretion stress and comparing these to those found in the yeast Saccharomyces cerevisiae. Results: Chemostat cultures of T. reesei expressing human tissue plasminogen activator (tPA) and batch bioreactor cultures treated with dithiothreitol (DTT) to prevent correct protein folding were analysed with cDNA subtraction and cDNA-amplified fragment length polymorphism (AFLP) experiments. ESTs corresponding to 457 unique genes putatively induced under secretion stress were isolated and the expression pattern of 60 genes vkas confirmed by Northern analysis. Expression of these genes was also studied in a strain over-expressing inositol-requiring enzyme I (IREI) protein, a sensor for the UPR pathway. To compare the data with that of S. cerevisiae, published transcriptome profiling data on various stress responses in S. cerevisiae was reanalysed. The genes up-regulated in response to secretion stress included a large number of secretion related genes in both organisms. In addition, analysis of T.reesei revealed up regulation of the cpcl transcription factor gene and nucleosomal genes. The induction of the cpcA and histone gene H4 were shown to be induced also in cultures of Aspergillus nidulans treated with DTT. Conclusion: Analysis of the genes induced under secretion stress has revealed novel features in the stress response in T. reesel and in filamentous fungi. We have demonstrated that in addition to the previously rather well characterised induction of genes for many ER proteins or secretion related proteins also other types of responses exist.

Original languageEnglish
Article number32
JournalBMC Genomics
Volume7
DOIs
Publication statusPublished - 20 Mar 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Trichoderma
Saccharomyces cerevisiae
Fungi
Genes
Unfolded Protein Response
Dithiothreitol
Complementary DNA
Aspergillus nidulans
Proteins
Batch Cell Culture Techniques
Secretory Pathway
Protein Folding
Expressed Sequence Tags
Gene Expression Profiling
Bioreactors
Tissue Plasminogen Activator
Inositol
Histones
Carrier Proteins
Transcription Factors

Cite this

@article{970fa3863bff400397a51ca5c8af54a0,
title = "Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae",
abstract = "Background: Secretion stress is caused by compromised folding, modification or transport of proteins in the secretory pathway. In fungi, induction of genes in response to secretion stress is mediated mainly by the unfolded protein response (UPR) pathway. This study aims at uncovering transcriptional responses occurring in the filamentous fungi Trichoderma reesei exposed to secretion stress and comparing these to those found in the yeast Saccharomyces cerevisiae. Results: Chemostat cultures of T. reesei expressing human tissue plasminogen activator (tPA) and batch bioreactor cultures treated with dithiothreitol (DTT) to prevent correct protein folding were analysed with cDNA subtraction and cDNA-amplified fragment length polymorphism (AFLP) experiments. ESTs corresponding to 457 unique genes putatively induced under secretion stress were isolated and the expression pattern of 60 genes vkas confirmed by Northern analysis. Expression of these genes was also studied in a strain over-expressing inositol-requiring enzyme I (IREI) protein, a sensor for the UPR pathway. To compare the data with that of S. cerevisiae, published transcriptome profiling data on various stress responses in S. cerevisiae was reanalysed. The genes up-regulated in response to secretion stress included a large number of secretion related genes in both organisms. In addition, analysis of T.reesei revealed up regulation of the cpcl transcription factor gene and nucleosomal genes. The induction of the cpcA and histone gene H4 were shown to be induced also in cultures of Aspergillus nidulans treated with DTT. Conclusion: Analysis of the genes induced under secretion stress has revealed novel features in the stress response in T. reesel and in filamentous fungi. We have demonstrated that in addition to the previously rather well characterised induction of genes for many ER proteins or secretion related proteins also other types of responses exist.",
author = "Mikko Arvas and Tiina Pakula and Karin Lanthaler and Markku Saloheimo and Mari Valkonen and Tapani Suortti and Geoff Robson and Merja Penttil{\"a}",
note = "CA2: TK402 PGN: 18 p.",
year = "2006",
month = "3",
day = "20",
doi = "10.1186/1471-2164-7-32",
language = "English",
volume = "7",
journal = "BMC Genomics",
issn = "1471-2164",

}

Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae. / Arvas, Mikko (Corresponding Author); Pakula, Tiina; Lanthaler, Karin; Saloheimo, Markku; Valkonen, Mari; Suortti, Tapani; Robson, Geoff; Penttilä, Merja.

In: BMC Genomics, Vol. 7, 32, 20.03.2006.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Common features and interesting differences in transcriptional responses to secretion stress in the fungi Trichoderma reesei and Saccharomyces cerevisiae

AU - Arvas, Mikko

AU - Pakula, Tiina

AU - Lanthaler, Karin

AU - Saloheimo, Markku

AU - Valkonen, Mari

AU - Suortti, Tapani

AU - Robson, Geoff

AU - Penttilä, Merja

N1 - CA2: TK402 PGN: 18 p.

PY - 2006/3/20

Y1 - 2006/3/20

N2 - Background: Secretion stress is caused by compromised folding, modification or transport of proteins in the secretory pathway. In fungi, induction of genes in response to secretion stress is mediated mainly by the unfolded protein response (UPR) pathway. This study aims at uncovering transcriptional responses occurring in the filamentous fungi Trichoderma reesei exposed to secretion stress and comparing these to those found in the yeast Saccharomyces cerevisiae. Results: Chemostat cultures of T. reesei expressing human tissue plasminogen activator (tPA) and batch bioreactor cultures treated with dithiothreitol (DTT) to prevent correct protein folding were analysed with cDNA subtraction and cDNA-amplified fragment length polymorphism (AFLP) experiments. ESTs corresponding to 457 unique genes putatively induced under secretion stress were isolated and the expression pattern of 60 genes vkas confirmed by Northern analysis. Expression of these genes was also studied in a strain over-expressing inositol-requiring enzyme I (IREI) protein, a sensor for the UPR pathway. To compare the data with that of S. cerevisiae, published transcriptome profiling data on various stress responses in S. cerevisiae was reanalysed. The genes up-regulated in response to secretion stress included a large number of secretion related genes in both organisms. In addition, analysis of T.reesei revealed up regulation of the cpcl transcription factor gene and nucleosomal genes. The induction of the cpcA and histone gene H4 were shown to be induced also in cultures of Aspergillus nidulans treated with DTT. Conclusion: Analysis of the genes induced under secretion stress has revealed novel features in the stress response in T. reesel and in filamentous fungi. We have demonstrated that in addition to the previously rather well characterised induction of genes for many ER proteins or secretion related proteins also other types of responses exist.

AB - Background: Secretion stress is caused by compromised folding, modification or transport of proteins in the secretory pathway. In fungi, induction of genes in response to secretion stress is mediated mainly by the unfolded protein response (UPR) pathway. This study aims at uncovering transcriptional responses occurring in the filamentous fungi Trichoderma reesei exposed to secretion stress and comparing these to those found in the yeast Saccharomyces cerevisiae. Results: Chemostat cultures of T. reesei expressing human tissue plasminogen activator (tPA) and batch bioreactor cultures treated with dithiothreitol (DTT) to prevent correct protein folding were analysed with cDNA subtraction and cDNA-amplified fragment length polymorphism (AFLP) experiments. ESTs corresponding to 457 unique genes putatively induced under secretion stress were isolated and the expression pattern of 60 genes vkas confirmed by Northern analysis. Expression of these genes was also studied in a strain over-expressing inositol-requiring enzyme I (IREI) protein, a sensor for the UPR pathway. To compare the data with that of S. cerevisiae, published transcriptome profiling data on various stress responses in S. cerevisiae was reanalysed. The genes up-regulated in response to secretion stress included a large number of secretion related genes in both organisms. In addition, analysis of T.reesei revealed up regulation of the cpcl transcription factor gene and nucleosomal genes. The induction of the cpcA and histone gene H4 were shown to be induced also in cultures of Aspergillus nidulans treated with DTT. Conclusion: Analysis of the genes induced under secretion stress has revealed novel features in the stress response in T. reesel and in filamentous fungi. We have demonstrated that in addition to the previously rather well characterised induction of genes for many ER proteins or secretion related proteins also other types of responses exist.

UR - http://www.scopus.com/inward/record.url?scp=33644889632&partnerID=8YFLogxK

U2 - 10.1186/1471-2164-7-32

DO - 10.1186/1471-2164-7-32

M3 - Article

C2 - 16504068

AN - SCOPUS:33644889632

VL - 7

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

M1 - 32

ER -