Communities of mold fungi in moisture damaged building materials

Elina Sohlberg, Hannu Viitanen

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsProfessional

Abstract

The critical conditions needed for the development of mould and decay fungi have been modelled for different building materials. However, current knowledge of indoor microbes growing on building materials relies on culture-based methods and more advanced molecular biological techniques should be employed to study the complex microbial communities in building materials. In this paper molecular biological techniques were optimized and used to study microbial diversity in building materials exposed to different moisture conditions. Different naturally contaminated and inoculated building materials were exposed to different humidity conditions (relative humidity 90% and 98%) in laboratory-scale experiment. The DNA extraction method was optimized to different building materials and microbial communities were studied by fungal ITS region targeted PCR-DGGE and sequencing. Fungal communities differed between building materials and humidity conditions. In RH 90% the majority of the sequences obtained belonged to genus Aspergillus. As expected, in RH 98% the fungal community was more diverse containing e.g. genera Penicillium, Aspergillus and Oidiodendron. The fungal diversity was highest in wood-based building materials
Original languageEnglish
Title of host publicationProceedings, IRG/WP 14-20542
Number of pages14
Publication statusPublished - 2014
MoE publication typeD3 Professional conference proceedings
Event45th annual meeting of the International Research Group on Wood Protection, IRG45 - St. George, United States
Duration: 11 May 201415 May 2014
Conference number: 45

Conference

Conference45th annual meeting of the International Research Group on Wood Protection, IRG45
Abbreviated titleIRG45
CountryUnited States
CitySt. George
Period11/05/1415/05/14

Keywords

  • Building materials
  • fungal communities
  • DNA sequencing
  • PCR-DGGE

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    Sohlberg, E., & Viitanen, H. (2014). Communities of mold fungi in moisture damaged building materials. In Proceedings, IRG/WP 14-20542