Abstract
α-L-Arabinofuranosidases (ABFs) are important enzymes in plant biomass degradation with a wide range of applications. The ascomycete fungus Penicillium subrubescens has more α-L-arabinofuranosidase-encoding genes in its genome compared to other Penicillia. We characterized nine ABFs from Glycoside Hydrolase (GH) families GH51, GH54 and GH62 from this fungus and demonstrated that they have highly diverse specificity and activity levels, indicating that the expansion was accompanied by diversification of the enzymes. Comparison of the substrate preference of the enzymes to the expression of the corresponding genes when the fungus was grown on either of two plant biomass substrates did not show a clear correlation, suggesting a more complex regulatory system governing L-arabinose release from plant biomass by P. subrubescens.
| Original language | English |
|---|---|
| Pages (from-to) | 360-368 |
| Journal | FEBS Letters |
| Volume | 596 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Feb 2022 |
| MoE publication type | A1 Journal article-refereed |
Funding
This study was supported by the National Council of Science and Technology of Mexico (CONACyT) for financial support (Grant No. 263888) to NCL. This work was partly supported by the China Scholarship Council (grant no: 201803250066) to XL.
Keywords
- Penicillium subrubescens
- arabinoxylan
- pectin
- recombinant expression
- α-l-arabinofuranosidases
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