Comparative gene expression profiles of intestinal transporters in mice, rats and humans

Hye Ryoung Kim, Sung Won Park, Hee Jung Cho, Kyung Ae Chae, Ji Min Sung, Jin Suk Kim, Christopher P. Landowski, Duxin Sun, A. M. Abd El-Aty, Gordon L. Amidon, Ho Chul Shin

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

We have studied gene expression profiles of intestinal transporters in model animals and humans. Total RNA was isolated from duodenum and the mRNA expression was measured using Affymetrix GeneChip oligonucleotide arrays. Detected genes from the intestine of mice, rats, and humans were about 60% of 22,690 sequences, 40% of 8739, and 47% of 12,559, respectively. A total of 86 genes involving transporters expressed in mice, 50 genes in rats, and 61 genes in humans were detected. Mice exhibited abundant mRNA expressions for peptide transporter HPT1, amino acid transporters CSNU3, CT1 and ASC1, nucleoside transporter CNT2, organic cation transporter SFXN1, organic anion transporter NBC3, glucose transporter SGLT1, and fatty acid transporters FABP1 and FABP2. Rats showed high expression profiles of peptide transporter PEPT1, amino acid transporters CSNU1 and 4F2HC, nucleoside transporter CNT2, organic cation transporter OCT5, organic anion transporter SDCT1, glucose transporter GLUT2 and GLUT5, and folate carrier FOLT. In humans, the highly expressed genes were peptide transporter HPT1, amino acid transporters LAT3, 4F2HC and PROT, nucleoside transporter CNT2, organic cation transporter OCTN2, organic anion transporters NADC1, NBC1 and SBC2, glucose transporters SGLT1 and GLUT5, multidrug resistance-associated protein RHO12, fatty acid transporters FABP1 and FABP2, and phosphate carrier PHC. Overall these data reveal diverse transcriptomic profiles for intestinal transporters among these species. Therefore, this transcriptional data may lead to more effective use of the laboratory animals as a model for oral drug development.

Original languageEnglish
Pages (from-to)224-236
Number of pages13
JournalPharmacological Research
Volume56
Issue number3
DOIs
Publication statusPublished - 1 Sep 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Transcriptome
Nucleoside Transport Proteins
Organic Anion Transporters
Amino Acid Transport Systems
Facilitative Glucose Transport Proteins
Genes
Cations
Fatty Acids
Phosphate Transport Proteins
Multidrug Resistance-Associated Proteins
Messenger RNA
Laboratory Animals
Oligonucleotide Array Sequence Analysis
Duodenum
Folic Acid
Intestines
Animal Models
RNA
Pharmaceutical Preparations
peptide permease

Keywords

  • Animals
  • Gene expression
  • Humans
  • Intestine
  • Transporters

Cite this

Kim, H. R., Park, S. W., Cho, H. J., Chae, K. A., Sung, J. M., Kim, J. S., ... Shin, H. C. (2007). Comparative gene expression profiles of intestinal transporters in mice, rats and humans. Pharmacological Research, 56(3), 224-236. https://doi.org/10.1016/j.phrs.2007.06.005
Kim, Hye Ryoung ; Park, Sung Won ; Cho, Hee Jung ; Chae, Kyung Ae ; Sung, Ji Min ; Kim, Jin Suk ; Landowski, Christopher P. ; Sun, Duxin ; Abd El-Aty, A. M. ; Amidon, Gordon L. ; Shin, Ho Chul. / Comparative gene expression profiles of intestinal transporters in mice, rats and humans. In: Pharmacological Research. 2007 ; Vol. 56, No. 3. pp. 224-236.
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abstract = "We have studied gene expression profiles of intestinal transporters in model animals and humans. Total RNA was isolated from duodenum and the mRNA expression was measured using Affymetrix GeneChip oligonucleotide arrays. Detected genes from the intestine of mice, rats, and humans were about 60{\%} of 22,690 sequences, 40{\%} of 8739, and 47{\%} of 12,559, respectively. A total of 86 genes involving transporters expressed in mice, 50 genes in rats, and 61 genes in humans were detected. Mice exhibited abundant mRNA expressions for peptide transporter HPT1, amino acid transporters CSNU3, CT1 and ASC1, nucleoside transporter CNT2, organic cation transporter SFXN1, organic anion transporter NBC3, glucose transporter SGLT1, and fatty acid transporters FABP1 and FABP2. Rats showed high expression profiles of peptide transporter PEPT1, amino acid transporters CSNU1 and 4F2HC, nucleoside transporter CNT2, organic cation transporter OCT5, organic anion transporter SDCT1, glucose transporter GLUT2 and GLUT5, and folate carrier FOLT. In humans, the highly expressed genes were peptide transporter HPT1, amino acid transporters LAT3, 4F2HC and PROT, nucleoside transporter CNT2, organic cation transporter OCTN2, organic anion transporters NADC1, NBC1 and SBC2, glucose transporters SGLT1 and GLUT5, multidrug resistance-associated protein RHO12, fatty acid transporters FABP1 and FABP2, and phosphate carrier PHC. Overall these data reveal diverse transcriptomic profiles for intestinal transporters among these species. Therefore, this transcriptional data may lead to more effective use of the laboratory animals as a model for oral drug development.",
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Kim, HR, Park, SW, Cho, HJ, Chae, KA, Sung, JM, Kim, JS, Landowski, CP, Sun, D, Abd El-Aty, AM, Amidon, GL & Shin, HC 2007, 'Comparative gene expression profiles of intestinal transporters in mice, rats and humans', Pharmacological Research, vol. 56, no. 3, pp. 224-236. https://doi.org/10.1016/j.phrs.2007.06.005

Comparative gene expression profiles of intestinal transporters in mice, rats and humans. / Kim, Hye Ryoung; Park, Sung Won; Cho, Hee Jung; Chae, Kyung Ae; Sung, Ji Min; Kim, Jin Suk; Landowski, Christopher P.; Sun, Duxin; Abd El-Aty, A. M.; Amidon, Gordon L.; Shin, Ho Chul.

In: Pharmacological Research, Vol. 56, No. 3, 01.09.2007, p. 224-236.

Research output: Contribution to journalArticleScientificpeer-review

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