Comparative nontargeted profiling of metabolic changes in tissues and biofluids in high-fat diet-fed Ossabaw pig

K. Hanhineva (Corresponding Author), T. Barri, M. Kolehmainen, J. Pekkinen, J. Pihlajamäki, A. Vesterbacka, G. Solano-Aguilar, H. Mykkänen, L.O. Dragsted, J.F. Urban, Kaisa Poutanen

Research output: Contribution to journalArticleScientificpeer-review

20 Citations (Scopus)

Abstract

Typical clinical biomarker analyses on urine and plasma samples from human dietary interventions do not provide adequate information about diet-induced metabolic changes taking place in tissues. The aim of this study was to show how a large-scale nontargeted metabolomic approach can be used to reveal metabolite groups for generating new hypotheses of obesity-related metabolic disturbances produced in an animal model. A large spectrum of metabolites in the semipolar region, including small water-soluble molecules like betaine and dihydroxyindole, and a wide range of bile acids as well as various lipid species were detected. The high-fat diet influenced metabolic homeostasis of Ossabaw pigs, especially the lipid metabolome, throughout all the analyzed sample types, including plasma, urine, bile, liver, pancreas, brain cortex, intestinal jejunum and proximal colon. However, even dramatic metabolic changes in tissues were not necessarily observed in plasma and urine. Metabolite profiling involving multiple sample types was shown to be a feasible method for the examination of a wide spectrum of metabolic species extending from small water-soluble metabolites to an array of bile acids and lipids, thus pointing to the pathways of metabolism affected by the dietary treatment.
Original languageEnglish
Pages (from-to)3980-3992
Number of pages12
JournalJournal of Proteome Research
Volume12
Issue number9
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fingerprint

High Fat Diet
Nutrition
Metabolites
Swine
Fats
Urine
Tissue
Bile Acids and Salts
Lipids
Plasmas
Betaine
Metabolomics
Metabolome
Water
Jejunum
Bile
Pancreas
Colon
Biomarkers
Homeostasis

Keywords

  • high-fat diet
  • metabolite profiling
  • metabolomics
  • nutrition
  • pig

Cite this

Hanhineva, K. ; Barri, T. ; Kolehmainen, M. ; Pekkinen, J. ; Pihlajamäki, J. ; Vesterbacka, A. ; Solano-Aguilar, G. ; Mykkänen, H. ; Dragsted, L.O. ; Urban, J.F. ; Poutanen, Kaisa. / Comparative nontargeted profiling of metabolic changes in tissues and biofluids in high-fat diet-fed Ossabaw pig. In: Journal of Proteome Research. 2013 ; Vol. 12, No. 9. pp. 3980-3992.
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abstract = "Typical clinical biomarker analyses on urine and plasma samples from human dietary interventions do not provide adequate information about diet-induced metabolic changes taking place in tissues. The aim of this study was to show how a large-scale nontargeted metabolomic approach can be used to reveal metabolite groups for generating new hypotheses of obesity-related metabolic disturbances produced in an animal model. A large spectrum of metabolites in the semipolar region, including small water-soluble molecules like betaine and dihydroxyindole, and a wide range of bile acids as well as various lipid species were detected. The high-fat diet influenced metabolic homeostasis of Ossabaw pigs, especially the lipid metabolome, throughout all the analyzed sample types, including plasma, urine, bile, liver, pancreas, brain cortex, intestinal jejunum and proximal colon. However, even dramatic metabolic changes in tissues were not necessarily observed in plasma and urine. Metabolite profiling involving multiple sample types was shown to be a feasible method for the examination of a wide spectrum of metabolic species extending from small water-soluble metabolites to an array of bile acids and lipids, thus pointing to the pathways of metabolism affected by the dietary treatment.",
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Hanhineva, K, Barri, T, Kolehmainen, M, Pekkinen, J, Pihlajamäki, J, Vesterbacka, A, Solano-Aguilar, G, Mykkänen, H, Dragsted, LO, Urban, JF & Poutanen, K 2013, 'Comparative nontargeted profiling of metabolic changes in tissues and biofluids in high-fat diet-fed Ossabaw pig', Journal of Proteome Research, vol. 12, no. 9, pp. 3980-3992. https://doi.org/10.1021/pr400257d

Comparative nontargeted profiling of metabolic changes in tissues and biofluids in high-fat diet-fed Ossabaw pig. / Hanhineva, K. (Corresponding Author); Barri, T.; Kolehmainen, M.; Pekkinen, J.; Pihlajamäki, J.; Vesterbacka, A.; Solano-Aguilar, G.; Mykkänen, H.; Dragsted, L.O.; Urban, J.F.; Poutanen, Kaisa.

In: Journal of Proteome Research, Vol. 12, No. 9, 2013, p. 3980-3992.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Comparative nontargeted profiling of metabolic changes in tissues and biofluids in high-fat diet-fed Ossabaw pig

AU - Hanhineva, K.

AU - Barri, T.

AU - Kolehmainen, M.

AU - Pekkinen, J.

AU - Pihlajamäki, J.

AU - Vesterbacka, A.

AU - Solano-Aguilar, G.

AU - Mykkänen, H.

AU - Dragsted, L.O.

AU - Urban, J.F.

AU - Poutanen, Kaisa

PY - 2013

Y1 - 2013

N2 - Typical clinical biomarker analyses on urine and plasma samples from human dietary interventions do not provide adequate information about diet-induced metabolic changes taking place in tissues. The aim of this study was to show how a large-scale nontargeted metabolomic approach can be used to reveal metabolite groups for generating new hypotheses of obesity-related metabolic disturbances produced in an animal model. A large spectrum of metabolites in the semipolar region, including small water-soluble molecules like betaine and dihydroxyindole, and a wide range of bile acids as well as various lipid species were detected. The high-fat diet influenced metabolic homeostasis of Ossabaw pigs, especially the lipid metabolome, throughout all the analyzed sample types, including plasma, urine, bile, liver, pancreas, brain cortex, intestinal jejunum and proximal colon. However, even dramatic metabolic changes in tissues were not necessarily observed in plasma and urine. Metabolite profiling involving multiple sample types was shown to be a feasible method for the examination of a wide spectrum of metabolic species extending from small water-soluble metabolites to an array of bile acids and lipids, thus pointing to the pathways of metabolism affected by the dietary treatment.

AB - Typical clinical biomarker analyses on urine and plasma samples from human dietary interventions do not provide adequate information about diet-induced metabolic changes taking place in tissues. The aim of this study was to show how a large-scale nontargeted metabolomic approach can be used to reveal metabolite groups for generating new hypotheses of obesity-related metabolic disturbances produced in an animal model. A large spectrum of metabolites in the semipolar region, including small water-soluble molecules like betaine and dihydroxyindole, and a wide range of bile acids as well as various lipid species were detected. The high-fat diet influenced metabolic homeostasis of Ossabaw pigs, especially the lipid metabolome, throughout all the analyzed sample types, including plasma, urine, bile, liver, pancreas, brain cortex, intestinal jejunum and proximal colon. However, even dramatic metabolic changes in tissues were not necessarily observed in plasma and urine. Metabolite profiling involving multiple sample types was shown to be a feasible method for the examination of a wide spectrum of metabolic species extending from small water-soluble metabolites to an array of bile acids and lipids, thus pointing to the pathways of metabolism affected by the dietary treatment.

KW - high-fat diet

KW - metabolite profiling

KW - metabolomics

KW - nutrition

KW - pig

U2 - 10.1021/pr400257d

DO - 10.1021/pr400257d

M3 - Article

VL - 12

SP - 3980

EP - 3992

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 9

ER -