Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes

Čeněk Novotný (Corresponding Author), Nicolina Dias, Anu Kapanen, Kateřina Malachová, Marta Vándrovcová, Merja Itävaara, Nelson Lima

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Abstract

Toxicity of two azo dyes (Reactive Orange 16 (RO16); Congo Red (CR)) and two anthraquinone dyes (Remazol Brilliant Blue R (RBBR); Disperse Blue 3 (DB3)) were compared using bacterium Vibrio fischeri, microalga Selenastrum capricornutum and ciliate Tetrahymena pyriformis. The following respective endpoints were involved: acute toxicity measured as bacterial luminescence inhibition, algal growth inhibition, and the effects on the protozoa including viability, growth inhibition, grazing effect and morphometric effects. In addition, mutagenicity of the dyes was determined using Ames test with bacterium Salmonella typhimurium His−. DB3 dye was the most toxic of all dyes in the bacterial, algal and protozoan tests. In contrast to other dyes, DB3 exhibited mutagenic effects after metabolic activation in vitro in all S. typhimurium strains used. Of the methods applied, the algal test was the most sensitive to evaluate toxicity of the dyes tested.
Original languageEnglish
Pages (from-to)1436-1442
JournalChemosphere
Volume63
Issue number9
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

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Keywords

  • Reactive Orange 16
  • Congo Red
  • Remazol Brilliant Blue R
  • Disperse Blue 3
  • Vibrio fischeri
  • Selenastrum capricornutum
  • Tetrahymena pyriformis

Cite this

Novotný, Č., Dias, N., Kapanen, A., Malachová, K., Vándrovcová, M., Itävaara, M., & Lima, N. (2006). Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes. Chemosphere, 63(9), 1436-1442. https://doi.org/10.1016/j.chemosphere.2005.10.002