Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes

Čeněk Novotný (Corresponding Author), Nicolina Dias, Anu Kapanen, Kateřina Malachová, Marta Vándrovcová, Merja Itävaara, Nelson Lima

Research output: Contribution to journalArticleScientificpeer-review

174 Citations (Scopus)

Abstract

Toxicity of two azo dyes (Reactive Orange 16 (RO16); Congo Red (CR)) and two anthraquinone dyes (Remazol Brilliant Blue R (RBBR); Disperse Blue 3 (DB3)) were compared using bacterium Vibrio fischeri, microalga Selenastrum capricornutum and ciliate Tetrahymena pyriformis. The following respective endpoints were involved: acute toxicity measured as bacterial luminescence inhibition, algal growth inhibition, and the effects on the protozoa including viability, growth inhibition, grazing effect and morphometric effects. In addition, mutagenicity of the dyes was determined using Ames test with bacterium Salmonella typhimurium His−. DB3 dye was the most toxic of all dyes in the bacterial, algal and protozoan tests. In contrast to other dyes, DB3 exhibited mutagenic effects after metabolic activation in vitro in all S. typhimurium strains used. Of the methods applied, the algal test was the most sensitive to evaluate toxicity of the dyes tested.
Original languageEnglish
Pages (from-to)1436-1442
JournalChemosphere
Volume63
Issue number9
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Azo Compounds
Anthraquinones
Toxicity
dye
Coloring Agents
Dyes
toxicity
Salmonella typhimurium
Bacteria
Aliivibrio fischeri
Protozoa
Tetrahymena pyriformis
Congo Red
Salmonella
Azo dyes
Poisons
bacterium
Growth
microalga
Luminescence

Keywords

  • Reactive Orange 16
  • Congo Red
  • Remazol Brilliant Blue R
  • Disperse Blue 3
  • Vibrio fischeri
  • Selenastrum capricornutum
  • Tetrahymena pyriformis

Cite this

Novotný, Č., Dias, N., Kapanen, A., Malachová, K., Vándrovcová, M., Itävaara, M., & Lima, N. (2006). Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes. Chemosphere, 63(9), 1436-1442. https://doi.org/10.1016/j.chemosphere.2005.10.002
Novotný, Čeněk ; Dias, Nicolina ; Kapanen, Anu ; Malachová, Kateřina ; Vándrovcová, Marta ; Itävaara, Merja ; Lima, Nelson. / Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes. In: Chemosphere. 2006 ; Vol. 63, No. 9. pp. 1436-1442.
@article{9ba570a2ed574e32825d1a7d9eba6eb9,
title = "Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes",
abstract = "Toxicity of two azo dyes (Reactive Orange 16 (RO16); Congo Red (CR)) and two anthraquinone dyes (Remazol Brilliant Blue R (RBBR); Disperse Blue 3 (DB3)) were compared using bacterium Vibrio fischeri, microalga Selenastrum capricornutum and ciliate Tetrahymena pyriformis. The following respective endpoints were involved: acute toxicity measured as bacterial luminescence inhibition, algal growth inhibition, and the effects on the protozoa including viability, growth inhibition, grazing effect and morphometric effects. In addition, mutagenicity of the dyes was determined using Ames test with bacterium Salmonella typhimurium His−. DB3 dye was the most toxic of all dyes in the bacterial, algal and protozoan tests. In contrast to other dyes, DB3 exhibited mutagenic effects after metabolic activation in vitro in all S. typhimurium strains used. Of the methods applied, the algal test was the most sensitive to evaluate toxicity of the dyes tested.",
keywords = "Reactive Orange 16, Congo Red, Remazol Brilliant Blue R, Disperse Blue 3, Vibrio fischeri, Selenastrum capricornutum, Tetrahymena pyriformis",
author = "Čeněk Novotn{\'y} and Nicolina Dias and Anu Kapanen and Kateřina Malachov{\'a} and Marta V{\'a}ndrovcov{\'a} and Merja It{\"a}vaara and Nelson Lima",
year = "2006",
doi = "10.1016/j.chemosphere.2005.10.002",
language = "English",
volume = "63",
pages = "1436--1442",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "Elsevier",
number = "9",

}

Novotný, Č, Dias, N, Kapanen, A, Malachová, K, Vándrovcová, M, Itävaara, M & Lima, N 2006, 'Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes', Chemosphere, vol. 63, no. 9, pp. 1436-1442. https://doi.org/10.1016/j.chemosphere.2005.10.002

Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes. / Novotný, Čeněk (Corresponding Author); Dias, Nicolina; Kapanen, Anu; Malachová, Kateřina; Vándrovcová, Marta; Itävaara, Merja; Lima, Nelson.

In: Chemosphere, Vol. 63, No. 9, 2006, p. 1436-1442.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Comparative use of bacterial, algal and protozoan tests to study toxicity of azo- and anthraquinone dyes

AU - Novotný, Čeněk

AU - Dias, Nicolina

AU - Kapanen, Anu

AU - Malachová, Kateřina

AU - Vándrovcová, Marta

AU - Itävaara, Merja

AU - Lima, Nelson

PY - 2006

Y1 - 2006

N2 - Toxicity of two azo dyes (Reactive Orange 16 (RO16); Congo Red (CR)) and two anthraquinone dyes (Remazol Brilliant Blue R (RBBR); Disperse Blue 3 (DB3)) were compared using bacterium Vibrio fischeri, microalga Selenastrum capricornutum and ciliate Tetrahymena pyriformis. The following respective endpoints were involved: acute toxicity measured as bacterial luminescence inhibition, algal growth inhibition, and the effects on the protozoa including viability, growth inhibition, grazing effect and morphometric effects. In addition, mutagenicity of the dyes was determined using Ames test with bacterium Salmonella typhimurium His−. DB3 dye was the most toxic of all dyes in the bacterial, algal and protozoan tests. In contrast to other dyes, DB3 exhibited mutagenic effects after metabolic activation in vitro in all S. typhimurium strains used. Of the methods applied, the algal test was the most sensitive to evaluate toxicity of the dyes tested.

AB - Toxicity of two azo dyes (Reactive Orange 16 (RO16); Congo Red (CR)) and two anthraquinone dyes (Remazol Brilliant Blue R (RBBR); Disperse Blue 3 (DB3)) were compared using bacterium Vibrio fischeri, microalga Selenastrum capricornutum and ciliate Tetrahymena pyriformis. The following respective endpoints were involved: acute toxicity measured as bacterial luminescence inhibition, algal growth inhibition, and the effects on the protozoa including viability, growth inhibition, grazing effect and morphometric effects. In addition, mutagenicity of the dyes was determined using Ames test with bacterium Salmonella typhimurium His−. DB3 dye was the most toxic of all dyes in the bacterial, algal and protozoan tests. In contrast to other dyes, DB3 exhibited mutagenic effects after metabolic activation in vitro in all S. typhimurium strains used. Of the methods applied, the algal test was the most sensitive to evaluate toxicity of the dyes tested.

KW - Reactive Orange 16

KW - Congo Red

KW - Remazol Brilliant Blue R

KW - Disperse Blue 3

KW - Vibrio fischeri

KW - Selenastrum capricornutum

KW - Tetrahymena pyriformis

U2 - 10.1016/j.chemosphere.2005.10.002

DO - 10.1016/j.chemosphere.2005.10.002

M3 - Article

VL - 63

SP - 1436

EP - 1442

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

IS - 9

ER -