Comparison of Chondroitin Sulfate and Hyaluronic Acid Doped Conductive Polypyrrole Films for Adipose Stem Cells

M. Björninen, A. Siljander, Jani Pelto, J. Hyttinen, M. Kellomäki, S. Miettinen, R. Seppänen, S. Haimi (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

18 Citations (Scopus)

Abstract

Polypyrrole (PPy) is a conductive polymer that has aroused interest due to its biocompatibility with several cell types and high tailorability as an electroconductive scaffold coating. This study compares the effect of hyaluronic acid (HA) and chondroitin sulfate (CS) doped PPy films on human adipose stem cells (hASCs) under electrical stimulation. The PPy films were synthetized electrochemically. The surface morphology of PPy-HA and PPy-CS was characterized by an atomic force microscope. A pulsed biphasic electric current (BEC) was applied via PPy films non-stimulated samples acting as controls. Viability, attachment, proliferation and osteogenic differentiation of hASCs were evaluated by live/dead staining, DNA content, Alkaline phosphatase activity and mineralization assays. Human ASCs grew as a homogenous cell sheet on PPy-CS surfaces, whereas on PPy-HA cells clustered into small spherical structures. PPy-CS supported hASC proliferation significantly better than PPy-HA at the 7 day time point. Both substrates equally triggered early osteogenic differentiation of hASCs, although mineralization was significantly induced on PPy-CS compared to PPy-HA under BEC. These differences may be due to different surface morphologies originating from the CS and HA dopants. Our results suggest that PPy-CS in particular is a potential osteogenic scaffold coating for bone tissue engineering
Original languageEnglish
Pages (from-to)1889-1900
JournalAnnals of Biomedical Engineering
Volume42
Issue number9
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Hyaluronic acid
Polypyrroles
Stem cells
Electric currents
Sulfates
Surface morphology
Coatings
Bioelectric potentials
Phosphatases
Cell proliferation
Scaffolds (biology)
Biocompatibility
Tissue engineering
Scaffolds
Assays

Keywords

  • mesenchymal stem cells
  • osteogenic
  • electrical simulation
  • polysaccharides

Cite this

Björninen, M. ; Siljander, A. ; Pelto, Jani ; Hyttinen, J. ; Kellomäki, M. ; Miettinen, S. ; Seppänen, R. ; Haimi, S. / Comparison of Chondroitin Sulfate and Hyaluronic Acid Doped Conductive Polypyrrole Films for Adipose Stem Cells. In: Annals of Biomedical Engineering. 2014 ; Vol. 42, No. 9. pp. 1889-1900.
@article{dc34fa9628754bb4a2f9ff12bde1950b,
title = "Comparison of Chondroitin Sulfate and Hyaluronic Acid Doped Conductive Polypyrrole Films for Adipose Stem Cells",
abstract = "Polypyrrole (PPy) is a conductive polymer that has aroused interest due to its biocompatibility with several cell types and high tailorability as an electroconductive scaffold coating. This study compares the effect of hyaluronic acid (HA) and chondroitin sulfate (CS) doped PPy films on human adipose stem cells (hASCs) under electrical stimulation. The PPy films were synthetized electrochemically. The surface morphology of PPy-HA and PPy-CS was characterized by an atomic force microscope. A pulsed biphasic electric current (BEC) was applied via PPy films non-stimulated samples acting as controls. Viability, attachment, proliferation and osteogenic differentiation of hASCs were evaluated by live/dead staining, DNA content, Alkaline phosphatase activity and mineralization assays. Human ASCs grew as a homogenous cell sheet on PPy-CS surfaces, whereas on PPy-HA cells clustered into small spherical structures. PPy-CS supported hASC proliferation significantly better than PPy-HA at the 7 day time point. Both substrates equally triggered early osteogenic differentiation of hASCs, although mineralization was significantly induced on PPy-CS compared to PPy-HA under BEC. These differences may be due to different surface morphologies originating from the CS and HA dopants. Our results suggest that PPy-CS in particular is a potential osteogenic scaffold coating for bone tissue engineering",
keywords = "mesenchymal stem cells, osteogenic, electrical simulation, polysaccharides",
author = "M. Bj{\"o}rninen and A. Siljander and Jani Pelto and J. Hyttinen and M. Kellom{\"a}ki and S. Miettinen and R. Sepp{\"a}nen and S. Haimi",
year = "2014",
doi = "10.1007/s10439-014-1023-7",
language = "English",
volume = "42",
pages = "1889--1900",
journal = "Annals of Biomedical Engineering",
issn = "0090-6964",
publisher = "Springer",
number = "9",

}

Björninen, M, Siljander, A, Pelto, J, Hyttinen, J, Kellomäki, M, Miettinen, S, Seppänen, R & Haimi, S 2014, 'Comparison of Chondroitin Sulfate and Hyaluronic Acid Doped Conductive Polypyrrole Films for Adipose Stem Cells', Annals of Biomedical Engineering, vol. 42, no. 9, pp. 1889-1900. https://doi.org/10.1007/s10439-014-1023-7

Comparison of Chondroitin Sulfate and Hyaluronic Acid Doped Conductive Polypyrrole Films for Adipose Stem Cells. / Björninen, M.; Siljander, A.; Pelto, Jani; Hyttinen, J.; Kellomäki, M.; Miettinen, S.; Seppänen, R.; Haimi, S. (Corresponding Author).

In: Annals of Biomedical Engineering, Vol. 42, No. 9, 2014, p. 1889-1900.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Comparison of Chondroitin Sulfate and Hyaluronic Acid Doped Conductive Polypyrrole Films for Adipose Stem Cells

AU - Björninen, M.

AU - Siljander, A.

AU - Pelto, Jani

AU - Hyttinen, J.

AU - Kellomäki, M.

AU - Miettinen, S.

AU - Seppänen, R.

AU - Haimi, S.

PY - 2014

Y1 - 2014

N2 - Polypyrrole (PPy) is a conductive polymer that has aroused interest due to its biocompatibility with several cell types and high tailorability as an electroconductive scaffold coating. This study compares the effect of hyaluronic acid (HA) and chondroitin sulfate (CS) doped PPy films on human adipose stem cells (hASCs) under electrical stimulation. The PPy films were synthetized electrochemically. The surface morphology of PPy-HA and PPy-CS was characterized by an atomic force microscope. A pulsed biphasic electric current (BEC) was applied via PPy films non-stimulated samples acting as controls. Viability, attachment, proliferation and osteogenic differentiation of hASCs were evaluated by live/dead staining, DNA content, Alkaline phosphatase activity and mineralization assays. Human ASCs grew as a homogenous cell sheet on PPy-CS surfaces, whereas on PPy-HA cells clustered into small spherical structures. PPy-CS supported hASC proliferation significantly better than PPy-HA at the 7 day time point. Both substrates equally triggered early osteogenic differentiation of hASCs, although mineralization was significantly induced on PPy-CS compared to PPy-HA under BEC. These differences may be due to different surface morphologies originating from the CS and HA dopants. Our results suggest that PPy-CS in particular is a potential osteogenic scaffold coating for bone tissue engineering

AB - Polypyrrole (PPy) is a conductive polymer that has aroused interest due to its biocompatibility with several cell types and high tailorability as an electroconductive scaffold coating. This study compares the effect of hyaluronic acid (HA) and chondroitin sulfate (CS) doped PPy films on human adipose stem cells (hASCs) under electrical stimulation. The PPy films were synthetized electrochemically. The surface morphology of PPy-HA and PPy-CS was characterized by an atomic force microscope. A pulsed biphasic electric current (BEC) was applied via PPy films non-stimulated samples acting as controls. Viability, attachment, proliferation and osteogenic differentiation of hASCs were evaluated by live/dead staining, DNA content, Alkaline phosphatase activity and mineralization assays. Human ASCs grew as a homogenous cell sheet on PPy-CS surfaces, whereas on PPy-HA cells clustered into small spherical structures. PPy-CS supported hASC proliferation significantly better than PPy-HA at the 7 day time point. Both substrates equally triggered early osteogenic differentiation of hASCs, although mineralization was significantly induced on PPy-CS compared to PPy-HA under BEC. These differences may be due to different surface morphologies originating from the CS and HA dopants. Our results suggest that PPy-CS in particular is a potential osteogenic scaffold coating for bone tissue engineering

KW - mesenchymal stem cells

KW - osteogenic

KW - electrical simulation

KW - polysaccharides

U2 - 10.1007/s10439-014-1023-7

DO - 10.1007/s10439-014-1023-7

M3 - Article

VL - 42

SP - 1889

EP - 1900

JO - Annals of Biomedical Engineering

JF - Annals of Biomedical Engineering

SN - 0090-6964

IS - 9

ER -