Abstract
Polypyrrole (PPy) is a conductive polymer that has
aroused interest due to its biocompatibility with several
cell types and high tailorability as an electroconductive
scaffold coating. This study compares the effect of
hyaluronic acid (HA) and chondroitin sulfate (CS) doped
PPy films on human adipose stem cells (hASCs) under
electrical stimulation. The PPy films were synthetized
electrochemically. The surface morphology of PPy-HA and
PPy-CS was characterized by an atomic force microscope. A
pulsed biphasic electric current (BEC) was applied via
PPy films non-stimulated samples acting as controls.
Viability, attachment, proliferation and osteogenic
differentiation of hASCs were evaluated by live/dead
staining, DNA content, Alkaline phosphatase activity and
mineralization assays. Human ASCs grew as a homogenous
cell sheet on PPy-CS surfaces, whereas on PPy-HA cells
clustered into small spherical structures. PPy-CS
supported hASC proliferation significantly better than
PPy-HA at the 7 day time point. Both substrates equally
triggered early osteogenic differentiation of hASCs,
although mineralization was significantly induced on
PPy-CS compared to PPy-HA under BEC. These differences
may be due to different surface morphologies originating
from the CS and HA dopants. Our results suggest that
PPy-CS in particular is a potential osteogenic scaffold
coating for bone tissue engineering
| Original language | English |
|---|---|
| Pages (from-to) | 1889-1900 |
| Journal | Annals of Biomedical Engineering |
| Volume | 42 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2014 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- mesenchymal stem cells
- osteogenic
- electrical simulation
- polysaccharides